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Screening antiacne potency of Indonesian medicinal plants: antibacterial, lipase inhibition, and antioxidant activities


Indonesian medicinal plants were screened as potential sources of antiacne agents. The screening methods were performed using antibacterial assay against Propionibacterium acnes, lipase inhibitor assay, and antioxidant assay. The results showed that from 40 plant materials extracted with methanol and 50% ethanol in water, Caesalpinia sappan was the best extract based on the combined activities: antibacterial (minimum inhibitory concentration 0.13 mg/ml; minimum bactericidal concentration 0.25 mg/ml), lipase inhibitory [50% inhibitory concentration (IC50) 120.0 μg/ml], and antioxidative (IC50 6.47 μg/ml). Another prospective extract is Intsia palembanica based on its lipase inhibitory activity (IC50 4.1 μg/ml) and antioxidant activity (IC50 3.87 μg/ml).


  1. Ghimire SK, McKey D, Thomas YA (2005) Conservation of Himalayan medicinal plants: harvesting patterns and ecology of two threatened species, Nardostachys grandiflora DC. and Neopicrorhiza scrophulariiflora (Pennell) Hong. Biol Conserv 124:463–475

    Article  Google Scholar 

  2. Brown SK, Shalita AR (1998) Acne vulgaris. Lancet 351:1871–1876

    Article  CAS  PubMed  Google Scholar 

  3. Toyoda M, Morohashi M (2001) Pathogens of acne. Med Electron Microsc 34:29–40

    Article  CAS  PubMed  Google Scholar 

  4. Katzman M, Logan AC (2007) Acne vulgaris: nutritional factors may be influencing psychological sequelae. Med Hypotheses 69:1080–1084

    Article  CAS  PubMed  Google Scholar 

  5. Nourin K, Ballard CJ (2006) Laser therapy for acne. Clin Dermatol 24:26–32

    Article  Google Scholar 

  6. Strauss JS, Krowchuk DP, Leyden JJ, Lucky AW, Shalita AR, Siegfried EC, Thiboutot DM, Van Voorrhees AS, Beutner KA, Sieck CK, Bhushan R (2007) Guidelines of care for acne vulgaris management. J Am Acad Dermatol 56:651–663

    Article  PubMed  Google Scholar 

  7. Zane LT (2005) Introduction: welcome to the next generation of acne research. Semin Cutan Med Surg 24:65–66

    Article  PubMed  Google Scholar 

  8. Burkhart CG, Burkhart CN, Lehmann PF (1999) Acne: a review of immunologic and microbiologic factors. Postgrad Med J 75:328–331

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Heymann WR (2006) Dialogue in dermatology: toll-like receptors in acne vulgaris. J Am Acad Dermatol 55:691–692

    Article  PubMed  Google Scholar 

  10. Higashi S (2003) Lipase inhibitors for the treatment of acne. J Mol Catal B Enzym 22:377–384

    Article  Google Scholar 

  11. Falcocchio S, Ruiz C, Pastor FIJ, Saso L, Diaz P (2006) Propionibacterium acnes GehA lipase, an enzyme involved in acne development, can be successfully inhibited by defined natural substances. J Mol Catal B Enzym 40:132–137

    Article  CAS  Google Scholar 

  12. Chomnawang MT, Surassmo S, Nukoolkarn VS, Gritsanapan W (2005) Antimicrobial effects of Thai medicinal plants against acneinducing bacteria. J Ethnopharmacol 101:330–333

    Article  PubMed  Google Scholar 

  13. Furukawa I, Kurooka S, Arisue K, Kohda K, Hayashi C (1982) Assays of serum lipase by the “BALB-DTNB Method” mechanized for use with discrete and continuous-flow analyzer. Clin Chem 26:110–113

    Google Scholar 

  14. Sangat HM, Zuhud EAM, Damayanti EK (2000) Kamus Penyakit dan Tumbuhan Obat Indonesia [Etnofi tomedika I] (in Indonesian). Yayasan Obor, Indonesia

    Google Scholar 

  15. Lim MY, Jeon JH, Jeong EY, Lee CH, Lee HS (2007) Antimicrobial activity of 5-hydroxy-1,4-naphtoquinone isolated from Caesalpinia sappan toward intestinal bacteria. Food Chem 100:1254–1258

    Article  CAS  Google Scholar 

  16. Xu HX, Lee SF (2004) The antibacterial principle of Caesalpinia sappan. Phytother Res 18:647–651

    Article  CAS  PubMed  Google Scholar 

  17. Ng LT, Yap SF (2003) Gynura Cass. In: Lemmens RHMJ, Bunyapraphatsara N (eds) Plant resources of South East Asia No 12 (3). Medicinal and poisonous plants 3. Prosea Foundation, Bogor, Indonesia, pp 231–233

    Google Scholar 

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Correspondence to Tohru Mitsunaga.

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Batubara, I., Mitsunaga, T. & Ohashi, H. Screening antiacne potency of Indonesian medicinal plants: antibacterial, lipase inhibition, and antioxidant activities. J Wood Sci 55, 230–235 (2009).

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