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Official Journal of the Japan Wood Research Society

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Antimicrobial activity against Streptococcus sobrinus and glucosyltransferase inhibitory activity of taxifolin and some flavanonol rhamnosides from kempas (Koompassia malaccensis) extracts

Journal of Wood Science volume 55, pages 308–313 (2009)Cite this article

Abstract

Twenty plant materials collected from the islands of Java and Kalimantan in Indonesia were extracted with 50% aqueous ethanol (crude extract). The crude extracts were assayed for antimicrobial activities against Streptococcus sobrinus and for glucosyltransferase (GTase) inhibition. Fourteen extracts inhibited the growth of S. sobrinus by more than 50% and six extracts inhibited GTase activity by more than 50% at a concentration of 100 μg/ml. Koompassia malaccensis (kempas) extracts showed 90% depression of S. sobrinus growth and 80% inhibition of GTase activity at a concentration of 100 μg/ml. Kempas crude extracts were subjected to column chromatography using Sephadex LH-20 and then preparative high-performance liquid chromatography to isolate four compounds A, B, C, and D. These compounds were identified as taxifolin and the flavanonol rhamnoside isomers neoastilbin, astilbin, and isoastilbin, respectively, from 1H and 13C nuclear magnetic resonance (NMR) spectra and other two-dimensional NMR techniques (COSY, HMBC, and HMQC). Each compound depressed the growth of S. sobrinus over a concentration range of 9.3242.7 μg/ml and showed GTase inhibitory activity with IC50 values in the range 27.4–57.3 μg/ml. Taxifolin and flavanonol rhamnoside isomers isolated for the first time from kempas could be potent compounds for preventing dental caries.

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Authors and Affiliations

  1. Department of Applied Life Science, Faculty of Biological Science, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Harlinda Kuspradini, Tohru Mitsunaga & Hideo Ohashi

  2. Department of Forest Product Technology, Faculty of Forestry, Mulawarman University, Samarinda, 75123, Indonesia

    Harlinda Kuspradini

Authors
  1. Harlinda Kuspradini
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  2. Tohru Mitsunaga
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  3. Hideo Ohashi
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Correspondence to Tohru Mitsunaga.

Additional information

Part of this report was presented at the 57th Annual Meeting of the Japan Wood Research Society Conference, Hiroshima, 2007

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Kuspradini, H., Mitsunaga, T. & Ohashi, H. Antimicrobial activity against Streptococcus sobrinus and glucosyltransferase inhibitory activity of taxifolin and some flavanonol rhamnosides from kempas (Koompassia malaccensis) extracts. J Wood Sci 55, 308–313 (2009). https://doi.org/10.1007/s10086-009-1026-4

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  • DOI: https://doi.org/10.1007/s10086-009-1026-4

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