Skip to main content

Official Journal of the Japan Wood Research Society

Journal of Wood Science Cover Image

Fluorescence lifetime imaging microscopy study of wood fibers


  1. 1.

    Olmstead JA, Gray DGJ (1993) Fluorescence emission from mechanical pulp sheets. J Photochem Photobiol A Chem 73: 59–65

    CAS  Article  Google Scholar 

  2. 2.

    Olmstead JA, Gray DGJ (1997) Fluorescence spectroscopy of cellulose, lignin and mechanical pulps. Pulp Paper Sci 23:571–581

    Google Scholar 

  3. 3.

    Castellan A, Davidson RSJ (1994) Steady state and dynamic fluorescence emission from Abies wood. J Photochem Photobiol A Chem 78:275–279

    CAS  Article  Google Scholar 

  4. 4.

    Castellan A, Choudhury H, Davidson R, Grelier SJ (1994) Comparative study of stone ground wood pulp and native wood 2. Application comparison of fluorescence of stone ground wood pulp and native wood. J Photochem Photobiol A Chem 81:117–122

    CAS  Article  Google Scholar 

  5. 5.

    Tylli H, Forsskåhl I, Olkkonen CJ (1995) The effect of photo irradiation on high yield pulps: spectroscopy and kinetics. J Photochem Photobiol A Chem 87:181–191

    CAS  Article  Google Scholar 

  6. 6.

    Machado EH, De Paula R, Ruggiero R, Gardrat C, Castellan AJ (2006) Photophysics of dibenzodioxocins. J Photochem Photobiol A Chem 180:165–174

    CAS  Article  Google Scholar 

  7. 7.

    Gadella TWJ, Clegg RM, Jovin TM (1994) Fluorescence lifetime imaging microscopy: pixel-by-pixel analysis of phase-modulation data. Bioimaging 2:139–159

    CAS  Article  Google Scholar 

  8. 8.

    Gadella TWJ, Jovin TM (1995) Oligomerization of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy. A stereochemical model for tyrosine kinase receptor activation. J Cell Biol 129:1543–1558

    CAS  Article  PubMed  Google Scholar 

  9. 9.

    van Munster EB, Gadella TWJ (2005) Fluorescence lifetime imaging microscopy (FLIM). In: Rietdorf J (ed) Microscopy techniques. Springer, Berlin Heidelberg New York, pp 143–175

    Google Scholar 

  10. 10.

    Squire A, Verveer PJ, Bastiaens PIH (2000) Multiple frequency fluorescence lifetime imaging microscopy. J Microsc 197:136–149

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Lakowicz JR (1999) Time domain lifetime measurements. In: Principles of fluorescence spectroscopy. Kluwer, New York, pp 98–154

    Google Scholar 

  12. 12.

    Terashima N, Fukushima K, He L, Takabe K (1993) Comprehensive model of the lignified plant cell wall. In: Jung HG, Buxton DR, Hatfield RD, Ralph J (eds) Forage cell wall and digestibility. American Society of Agronomy, Madison, WI, pp 247–270

    Google Scholar 

  13. 13.

    Hafrén J (2007) Excitation wavelength specific changes in lignocellulosic autofluorescence. J Wood Sci 53:358–360

    Article  Google Scholar 

  14. 14.

    Córdova A, Hafrén J (2005) Direct organic acid catalyzed polyester derivatization of lignocellulosic material. Nord Pulp Paper Res J 20:477–480

    Article  Google Scholar 

  15. 15.

    Radotić K, Kalauzi A, Djikanović D, Jeremic M, Leblanc RM, Cerović ZG (2006) Component analysis of the fluorescence spectra of a lignin model compound. J Photochem Photobiol B Biol 83:1–10

    Article  Google Scholar 

  16. 16.

    Paulsson M, Ragauskas AJ (1998) Chemical modification of lignin-rich paper. Part 10. The light-induced yellowing of untreated and acetylated high-yield pulps studied by solid state UV/VIS diffuse reflectance spectroscopy. Nord Pulp Paper Res J 13:198–205

    CAS  Article  Google Scholar 

  17. 17.

    Miyawaki A, Griesbeck O, Heim R, Tsien RY (1999) Dynamic and quantitative Ca2 measurements using improved cameleons. Proc Natl Acad Sci USA 96:2135–40

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  18. 18.

    Lin HJ, Herman P, Lakowicz JR (2003) Fluorescence lifetime-resolved pH imaging of living cells. Cytometry 52A:77–89

    Article  Google Scholar 

Download references

Author information



Corresponding author

Correspondence to Jonas Hafrén.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hafrén, J., Oosterveld-Hut, H.M.J. Fluorescence lifetime imaging microscopy study of wood fibers. J Wood Sci 55, 236–239 (2009).

Download citation

Key words

  • Fluorescence
  • Lifetime
  • Lignin
  • Microscopy
  • Wood