- Original Article
- Published:
Extracellular peroxidase reaction at hyphal tips of white-rot fungus Phanerochaete crassa WD1694 and in fungal slime
Journal of Wood Science volume 55, pages 302–307 (2009)
Abstract
The distribution of an extracellular peroxidase reaction by white-rot fungus Phanerochaete crassa WD1694 was visualized by peroxidase activity staining. The extracellular peroxidase reaction occurred at the hyphal tips and in the fungal slime filling the gaps between the hyphae. We investigated whether the peroxidase reaction occurred from the hyphal tips or in the slime. The hyphal tips were observed by phase-contrast microscopy, which showed that slime did not exist around the hyphal tips. Time-course observation of hyphal tips showed that peroxidase staining became thick and intense at the tips that did not have fungal slime. Daily observation of the peroxidase staining revealed that the staining was first observed at the hyphal tips. Furthermore, strongly stained hyphae were observed in the stained slime. These results suggested that an active species that oxidizes a peroxidase substrate is first produced at the tips of the hyphae, and then occurs in the slime via diffusion when slime exists around the hyphae. Our results show that the extracellular peroxidase reaction that is important to lignin biodegradation by white-rot fungi occurs directly at the tips of the hyphae and in the slime.
References
Nakano J (1979) Lignin in plants (in Japanese). In: Nakano J (ed) Chemistry of lignin, basic science and application. Uni-shuppan, Tokyo, pp 20–36
Higuchi T (1985) Biosynthesis of lignin. In: Higuchi T (ed) Biosynthesis and biodegradation of wood components. Academic, London, pp 141–161
Chen CL, Chang HM (1985) Chemistry of lignin biodegradation. In: Higuchi T (ed) Biosynthesis and biodegradation of wood components. Academic, London, pp 535–556
Eriksson KEL, Blanchette RA, Ander P (1990) Microbial and enzymatic degradation of wood and wood components. Springer, Berlin Heidelberg New York, pp 234–249
Glenn JK, Morgan MA, Mayfi eld MB, Kuwahara M, Gold MH (1983) An extracellular H2O2-requiring enzyme preparation involved in lignin biodegradation by the white rot basidiomycete Phanerochaete chrysosporium. Biochem Biophys Res Commun 114:1077–1083
Tien M, Kirk TK (1983) Lignin-degrading enzyme from the hymenomycete Phanerochaete chrysosporium burds. Science 221: 661–663
Kirk TK (1987) Enzymatic “combustion”: the microbial degradation of lignin. Ann Rev Microbiol 41:465–505
Gold MH, Wariishi H, Valli K (1989) Extracellular peroxidases involved in lignin degradation by the white rot basidiomycete Phanerochaete chrysosporium. ACS Symp Ser 389:127–140
Higuchi T (1985) Degradative pathways of lignin model compounds. In: Higuchi T (ed) Biosynthesis and biodegradation of wood components. Academic, London, pp 557–578
Daniel G (1994) Use of electron microscopy for aiding our understanding of wood biodegradation. FEMS Microbiol Rev 13: 199–233
Daniel G, Nilsson T, Pettersson B (1989) Intra- and extracellular localization of lignin peroxidase during the degradation of solid wood and wood fragments by Phanerochaete chrysosporium by using transmission electron microscopy and immuno-gold labeling. Appl Environ Microbiol 55:871–881
Joseleau JP, Ruel K (1992) Ultrastructural examination of lignin and polysaccharide degradation in wood by white-rot fungi. Proceedings of 5th International Conference on Biotechnology in the Pulp and Paper Industry, Kyoto, May 27–30, pp 195–201
Daniel G, Pettersson B, Volc J, Nilsson T (1990) Spatial distribution of lignin- and manganese peroxidase(s) during degradation of wood and wood fragments by Phanerochaete chrysosporium as revealed by TEM immunogold labeling. In: Kirk TK, Chang H-M (eds) Biotechnology in pulp and paper manufacture. Applications and fundamental investigations. Butterworth-Heinemann, Boston, pp 99–110
Takano M, Abe H, Hayashi N (2006) Extracellular peroxidase activity at the hyphal tips of the white-rot fungus Phanerochaete crassa WD1694. J Wood Sci 52:429–435
Palmer JG, Muranis L, Highly TL (1983) Visualization of hyphal sheath in wood-decay hymenomycetes. II. White-rotters. Mycologia 75:1005–1010
Palmer JG, Muranis L, Highly TL (1983) Visualization of hyphal sheath in wood-decay hymenomycetes. I. Brown-rotters. Mycologia 75:995–1004
Abu AR, Murphy RJ, Dickinson DJ (1999) Investigation of the extracellular mucilaginous materials produced by some wood decay fungi. Mycol Res 103:1453–1461
Takano M, Hayashi N, Kuroda K (2008) Selective staining and visualization of hyphal sheath of a white-rot fungus Phanerochaete crassa WD1694 with Phloxine B. J Wood Sci 54:76–80
Author information
Authors and Affiliations
Corresponding author
Additional information
Part of this report was presented at the 50th Lignin Symposium, October 19–20, 2005, Nagoya, Japan
Rights and permissions
About this article
Cite this article
Takano, M., Hayashi, N., Nakamura, M. et al. Extracellular peroxidase reaction at hyphal tips of white-rot fungus Phanerochaete crassa WD1694 and in fungal slime. J Wood Sci 55, 302–307 (2009). https://doi.org/10.1007/s10086-009-1030-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10086-009-1030-8