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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Proteomic analysis of the G-layer in poplar tension wood

Journal of Wood Science volume 55, pages 250–257 (2009)Cite this article

Abstract

Angiosperm trees bend their stems by forming tension wood at the upper side of leaning stems. Most tension wood has a cellulose-rich G-layer in the innermost surface of the fiber cell wall. Strong tensile stress is considered to occur in the G-layer. This study undertook to identify the proteins involved in G-layer formation and function through a proteomic analysis of G-layer-localized protein. G-layers of poplar were loosened by sonication and isolated as doughnut-shaped pieces of thinly sliced transverse sections. The proteins, once extracted with urea/detergent solution, were separated by two-dimensional polyacrylamide gel electrophoresis, and 110 spots were subjected to liquid chromatography tandem mass spectrometry (LC/MS/MS). A database search for these spots’ mass spectrum patterns identified 72 proteins. In addition, all peptide digestion mixtures of G-layer proteins were separated by strong cation exchange chromatography and 39 proteins were identified using LC/MS/MS analysis. Proteins involved in wall formation, such as lignin biosynthesis-related protein, xyloglucan endotransglucosylase, and fasciclin-like arabinogalactan protein, were notably detected in the G-layer.

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Authors and Affiliations

  1. Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto, 611-0011, Japan

    Tomomi Kaku, Kei’ichi Baba, Fumio Tanaka & Takahisa Hayashi

  2. National Institute of Biomedical Innovation, Ibaraki, Osaka, 567-0085, Japan

    Satoshi Serada

Authors
  1. Tomomi Kaku
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  2. Satoshi Serada
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  3. Kei’ichi Baba
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  4. Fumio Tanaka
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  5. Takahisa Hayashi
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Correspondence to Takahisa Hayashi.

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Kaku, T., Serada, S., Baba, K. et al. Proteomic analysis of the G-layer in poplar tension wood. J Wood Sci 55, 250–257 (2009). https://doi.org/10.1007/s10086-009-1032-6

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  • DOI: https://doi.org/10.1007/s10086-009-1032-6

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