Skip to main content
Journal of Wood Science

Official Journal of the Japan Wood Research Society

Download PDF
  • Original Article
  • Published:

Ethanol production with β-xylosidase, xylose isomerase, and Saccharomyces cerevisiae from the hydrolysate of Japanese beech after hot-compressed water treatment

Journal of Wood Science volume 55, pages 289–294 (2009)Cite this article

Abstract

Ethanol was produced from the hydrolysate collected as the water-soluble (WS) portion after hot-compressed water (HCW) treatment of Japanese beech. The process involved saccharification with β-xylosidase followed by isomerization with xylose isomerase and fermentation with Saccharomyces cerevisiae. Several process schemes were compared to investigate the effect of process integration of saccharification, isomerization, and fermentation. Higher ethanol yields were obtained for the processes that integrated isomerization and fermentation or saccharification and isomerization. Integration of isomerization and fermentation was effective in converting xylose into ethanol. Similarly, integration of saccharification and isomerization was effective in converting xylooligosaccharides into xylulose. It is presumed that the saccharification reaction toward xylose and the isomerization reaction toward xylulose were linked and therefore each reaction was enhanced.

References

  1. Olsson L, Hahn-Hägerdal B (1996) Fermentation of lignocellulosic hydrolysates for ethanol production. Enzym Microb Technol 18:312–331

    Article  CAS  Google Scholar 

  2. Olivier SP, du Toit PJ (1986) Sugar cane bagasse as a possible source of fermentable carbohydrates. II. Optimization of the xylose isomerase reaction for isomerization of xylose as well as sugar cane bagasse hydrolyzate to xylulose in laboratory-scale units. Biotechnol Bioeng 28:684–699

    Article  CAS  PubMed  Google Scholar 

  3. du Preez JC (1994) Process parameters and environmental factors affecting D-xylose fermentation by yeasts. Enzym Microb Technol 16:944–956

    Article  Google Scholar 

  4. Linden T, Hahn-Hägerdal B (1989) Fermentation of lignocellulose hydrolysates with yeasts and xylose isomerase. Enzym Microb Technol 11:583–589

    Article  CAS  Google Scholar 

  5. Saka S, Ueno T (1999) Chemical conversion of various celluloses to glucose and its derivatives in supercritical water. Cellulose 6:177–191

    Article  CAS  Google Scholar 

  6. Ehara K, Saka S (2002) A comparative study on chemical conversion of cellulose between the batch-type and flow-type systems in supercritical water. Cellulose 9:301–311

    Article  CAS  Google Scholar 

  7. Nakata T, Miyafuji H, Saka S (2009) Enzymatic saccharification of the water-soluble portion after hot-compressed water treatment of Japanese beech with xylanase and β-xylosidase. J Wood Sci 55:209–214

    Article  CAS  Google Scholar 

  8. Suihko ML, Poutanen K (1984) D-Xylulose fermentation by free and immobilized Saccharomyces cerevisiae cells. Biotechnol Lett 6:189–194

    Article  CAS  Google Scholar 

  9. Linden T, Peetre J, Hahn-Hägerdal B (1992) Isolation and characterization of acetic acid-tolerant galactose-fermenting strains of Saccharomyces cerevisiae from a spent sulfite liquor fermentation plant. Appl Environ Microbiol 58:1661–1669

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Chiang LC, Gong CS, Chen LF, Tsao GT (1981) D-Xylulose fermentation to ethanol by Saccharomyces cerevisiae. Appl Environ Microbiol 42:284–289

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Takenishi S, Tsujisaka Y, Fukumoto J (1973) Purification and properties of the β-xylosidase produced by Aspergillus niger van Tieghem. J Biochem 73:335–343

    CAS  PubMed  Google Scholar 

  12. Poutanen K, Rättü M, Puls J, Viikari L (1987) Evaluation of different microbial xylanolytic systems. J Biotechnol 6:49–60

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Socio-Environmental Energy Science, Graduate School of Energy Science, Kyoto University, Yoshida Hon-machi, Sakyo-ku, Kyoto, 606-8501, Japan

    Toshiki Nakata, Hisashi Miyafuji & Shiro Saka

Authors
  1. Toshiki Nakata
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hisashi Miyafuji
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shiro Saka
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shiro Saka.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nakata, T., Miyafuji, H. & Saka, S. Ethanol production with β-xylosidase, xylose isomerase, and Saccharomyces cerevisiae from the hydrolysate of Japanese beech after hot-compressed water treatment. J Wood Sci 55, 289–294 (2009). https://doi.org/10.1007/s10086-009-1033-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10086-009-1033-5

Key words