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Official Journal of the Japan Wood Research Society

Journal of Wood Science Cover Image
  • Original Article
  • Open Access

Screening for condensed tannin-degrading fungi with a synthetic 14C-labeled compound

Journal of Wood ScienceOfficial Journal of the Japan Wood Research Society200955:1035

https://doi.org/10.1007/s10086-009-1035-3

  • Received: 8 December 2008
  • Accepted: 22 April 2009
  • Published:

Abstract

To find fungi that are potent for degradation of condensed tannin, a two-step screening was used. This involved measurement of fungal growth rate on Japanese cedar (Cryptomeria japonica) bark, followed by determination of [14C]-labeled CO2 generated from fungal degradation of synthetic [14C]-labeled condensed tannin model. In the first screening, 75 strains of wood rot fungi were tested, and 19 strains effectively decreased bark weight and/or the weight of the methanol-soluble fraction. For the second screening, [14C]-labeled condensed tannin model compound was synthesized in 11.8% yield based on radioactivity measurements. Over the incubation period, Coriolus hirsutus K-2671, Lentinus edodes Is, and Lampteromyces japonicus Nn showed higher cumulative [14C]-labeled CO2 emissions than the other strains and mineralized the [14C]-labeled condensed tannin model compound by 3.7%, 3.0%, and 3.0%, respectively. Fractionation of the methanol extracts from the medium by gel permeation chromatography after fungal treatment suggested that fungi that can induce the emission of significant levels of [14C]-labeled CO2 can extensively depolymerize condensed tannins.

Key words

  • Condensed tannins
  • Biodegradation
  • Fungi

Notes

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