Skip to main content
Journal of Wood Science

Official Journal of the Japan Wood Research Society

Download PDF

Volume 55 Supplement 6

Special Issue on Wood Science and Technology for Mitigation of Global Warming

  • Original Article
  • Published:

Enhancement of saccharification by overexpression of poplar cellulase in sengon

Journal of Wood Science volume 55, pages 435–440 (2009)Cite this article

Abstract

Lignocellulosic material from trees has great potential to form the basis of the second generation for bioethanol production because trees produce most of the biomass on the earth. We modified the wall structure of sengon (Paraserianthes falcataria) through overexpression of poplar cellulase in the cell walls. The overexpression did not decrease cellulose content but caused a decrease in xyloglucan bound to the walls. The level of saccharification and successive ethanol production was increased in the wood of the transgenic sengon overexpressing poplar cellulase compared with that of the wild type plant, and even after delignification of the wood. We propose that a xyloglucan intercalated into cellulose microfibrils could be one of the recalcitrant components in the saccharification of lignocelluloses.

References

  1. Najafi G, Ghobadian B, Tavakoli T, Buttsworth DR, Yusaf TF, Faizollahnejad M (2009) Performance and exhaust emissions of a gasoline engine with ethanol blended gasoline fuels using artificial neural network. Appl Energ 86:630–639

    Article  CAS  Google Scholar 

  2. Gray KA, Zhao L, Emptage M (2006) Bioethanol. Curr Opin Chem Biol 10:141–146

    Article  CAS  PubMed  Google Scholar 

  3. Lin Y, Tanaka S (2006) Ethanol fermentation from biomass resources: current state and prospects. Appl Microbiol Biotechnol 69:627–642

    Article  CAS  PubMed  Google Scholar 

  4. Ragauskas AJ, Williams CK, Davison BH, Britovsek G, Cairney J, Eckert CA, Frederick WJ Jr, Hallett JP, Leak DJ, Liotta CL, Mielenz JR, Murphy R, Templer R, Tschaplinski T (2006) The path forward for biofuels and biomaterials. Science 311:484–489

    Article  CAS  PubMed  Google Scholar 

  5. Kaida R, Kaku T, Baba K, Hartati S, Sudarmonowati E, Hayashi T (2009) Enzymatic saccharification and ethanol production of Acacia mangium and Paraserianthes falcataria wood, and Elaeis guineensis trunk. J Wood Sci. DOI 10.100T/s10086-009-1038-0

  6. Huang H-J, Ramaswamy S, Al-Dajani W, Tschimer U, Cairncross RA (2009) Effect of biomass species and plant size on cellulosic ethanol: a comparative process and economic analysis. Biomass Bioenerg 33:234–246

    Article  CAS  Google Scholar 

  7. Binkley D, Senock R, Bird S, Cole TG (2003) Twenty years of stand development in pure and mixed stands of Eucalyptus saligna and nitrogen fixing Falcataria moluccana. Forest Ecol Manag 182:93–102

    Article  Google Scholar 

  8. Nakamura S, Hayashi T (1993) Purification and properties of an extracellular endo-1,4-β-glucanase from suspension-cultured poplar cells. Plant Cell Physiol 34:1009–1013

    CAS  Google Scholar 

  9. Ohmiya Y, Samejima M, Shiroishi M, Amano Y, Kanda T, Sakai F, Hayashi T (2000) Evidence that endo-1,4-β-glucanase act on cellulose in suspension-cultured poplar cells. Plant J 24:147–158

    Article  CAS  PubMed  Google Scholar 

  10. Hartati S, Sudarmonowati E, Park YP, Kaku T, Kaida R, Baba K, Hayashi T (2008) Overexpression of poplar cellulase accelerates growth and disturbs the closing movements of leaves in sengon. Plant Physiol 147:552–561

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Mitsuhara I, Ugaki M, Hirochika H, Ohshima M, Murakami T, Gotoh Y, Katayose Y, Nakamura S, Honkura R, Nishimiya S, Ueno K, Mochizuki A, Tanimoto H, Tsugawa H, Otsuki Y, Ohashi Y (1996) Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants. Plant Cell Physiol 37:49–59

    Article  CAS  PubMed  Google Scholar 

  12. Updegraff DM (1969) Semimicro determination of cellulose in biological materials. Anal Chem 32:420–424

    CAS  Google Scholar 

  13. Hayashi T (1989) Measuring β-glucan deposition in plant cell walls. In: Linskens HF, Jackson JF (eds) Modern methods of plant analysis: plant fibers. Springer, Berlin Heidelberg New York, pp 138–160

    Google Scholar 

  14. Chiang VL, Funaoka M (1990) The difference between guaiacyl and guaiacyl-syringyl lignins in their responses to kraft delignification. Holzforschung 44:309–313

    Article  CAS  Google Scholar 

  15. Miyafuji H, Nakata T, Ehara K, Saka S (2005) Fermentability of water-soluble portion to ethanol obtained by supercritical water treatment of lignocellulosics. Appl Biochem Biotechnol 121-124:963–971

    Article  CAS  PubMed  Google Scholar 

  16. Park YW, Tominaga R, Sugiyama J, Furuta Y, Tanimoto E, Samejima M, Sakai F, Hayashi T (2003) Enhancement of growth by expression of poplar cellulase in Arabidopsis thaliana. Plant J 33:1099–1106

    Article  CAS  PubMed  Google Scholar 

  17. Shani Z, Dekel M, Tsabary G, Goren R, Shoseyov O (2004) Growth enhancement of transgenic poplar plants by overexpression of Arabidopsis thaliana endo-1,4-β-glucanase (cel1). Mol Breed 14:321–330

    Article  Google Scholar 

  18. Park YW, Baba K, Furuta Y, Iida I, Sameshima K, Arai M, Hayashi T (2004) Enhancement of growth and cellulose accumulation by overexpression of xyloglucanase in poplar. FEBS Lett 564:183–187

    Article  CAS  PubMed  Google Scholar 

  19. Nishikubo N, Awano T, Banasiak A, Bourquin V, Ibatullin F, Funada R, Brummer H, Teeri TT, Hayashi T, Sundberg B, Mellerowicz EJ (2007) Xyloglucan endo-transglycosylase (XET) functions in gelatinous layers of tension wood fibers in poplar - a glimpse into the mechanism of the balancing act of trees. Plant Cell Physiol 48:843–855

    Article  Google Scholar 

  20. Baba K, Park YW, Kaku T, Kaida R, Takeuchi M, Yoshida M, Hosoo Y, Ojio Y, Okuyama T, Taniguchi T, Ohmiya Y, Kondo T, Shani Z, Shoseyov O, Awano T, Serada S, Norioka N, Norioka S, Hayashi T (2009) Xyloglucan for generating tensile stress to bend tree stem. Mol Plant. doi:10.1093/mp/ssp054

  21. Taniguchi T, Omiya Y, Kurita M, Tsubomura M, Kondo T, Park YW, Baba K, Hayashi T (2008) Biosafty assessment of transgenic poplars overexpressing xyloglucanase (AaXEG2) prior to field trials. J Wood Sci 54:408–413

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan

    Rumi Kaida, Tomomi Kaku, Kei’ichi Baba & Takahisa Hayashi

  2. Research Centre for Biotechnology, LIPI, Cibinong, 16911, Indonesia

    Sri Hartati & Enny Sudarmonowati

Authors
  1. Rumi Kaida
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tomomi Kaku
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kei’ichi Baba
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sri Hartati
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Enny Sudarmonowati
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Takahisa Hayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rumi Kaida.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kaida, R., Kaku, T., Baba, K. et al. Enhancement of saccharification by overexpression of poplar cellulase in sengon. J Wood Sci 55, 435–440 (2009). https://doi.org/10.1007/s10086-009-1048-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10086-009-1048-y

Keywords