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Official Journal of the Japan Wood Research Society

The biological activities of cardenolide triglycosides from stems, twigs, and leaves of Nerium oleander

Abstract

Sixteen cardenolide triglycosides (116) were isolated from stems, twigs, and leaves of Nerium oleander. Among them, 3β-O-(4-O-gentiobiosyl-d-diginosyl)-7β,8-epoxy-14-hydroxy-5β,14β-card-20(22)-enolide, named cardenolide B-3 (16), was isolated from natural sources for the first time. The in vitro anti-inflammatory activities of compounds 116 were examined on the basis of inhibitory activity against the induction of intercellular adhesion molecule-1 (ICAM-1). Compounds 15 were active at an IC50 value of less than 7 μM. The cytotoxic activity of isolated compounds was evaluated against three human cell lines: normal human fibroblast cells (W-38), malignant tumor cells induced from WI-38 (VA-13), and human liver tumor cells (HepG2). Compounds 15 were active toward WI-38 cells; compounds 1, 3, and 5 were active toward VA-13 cells; and compounds 15 were active toward HepG2 cells at IC50 values of less than 10 μM. The multidrug-resistant (MDR) cancer-reversal activity of compounds 116 was evaluated on the basis of the amount of calcein accumulated in MDR human ovarian cancer 2780AD cells in the presence of each compound. Compounds 13 and 14 showed significant effects on calcein accumulation.

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Correspondence to Yuhua Bai.

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Bai, Y., Zhao, M., Bai, L. et al. The biological activities of cardenolide triglycosides from stems, twigs, and leaves of Nerium oleander . J Wood Sci 57, 56–65 (2011). https://doi.org/10.1007/s10086-010-1132-3

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  • DOI: https://doi.org/10.1007/s10086-010-1132-3

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