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Official Journal of the Japan Wood Research Society

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The biological activities of cardenolide triglycosides from stems, twigs, and leaves of Nerium oleander

Journal of Wood Science volume 57, pages 56–65 (2011)Cite this article

Abstract

Sixteen cardenolide triglycosides (1–16) were isolated from stems, twigs, and leaves of Nerium oleander. Among them, 3β-O-(4-O-gentiobiosyl-d-diginosyl)-7β,8-epoxy-14-hydroxy-5β,14β-card-20(22)-enolide, named cardenolide B-3 (16), was isolated from natural sources for the first time. The in vitro anti-inflammatory activities of compounds 1–16 were examined on the basis of inhibitory activity against the induction of intercellular adhesion molecule-1 (ICAM-1). Compounds 1–5 were active at an IC50 value of less than 7 μM. The cytotoxic activity of isolated compounds was evaluated against three human cell lines: normal human fibroblast cells (W-38), malignant tumor cells induced from WI-38 (VA-13), and human liver tumor cells (HepG2). Compounds 1–5 were active toward WI-38 cells; compounds 1, 3, and 5 were active toward VA-13 cells; and compounds 1–5 were active toward HepG2 cells at IC50 values of less than 10 μM. The multidrug-resistant (MDR) cancer-reversal activity of compounds 1–16 was evaluated on the basis of the amount of calcein accumulated in MDR human ovarian cancer 2780AD cells in the presence of each compound. Compounds 13 and 14 showed significant effects on calcein accumulation.

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Authors and Affiliations

  1. Department of Pharmacy, Daqing Campus of Harbin Medical University, Daqing Hi-Tech Development Zone, Xinyang Road, Daqing, 163319, China

    Yuhua Bai

  2. College of Chemistry and Chemistry Engineering, Qiqihar University, Qiqihar, 161006, China

    Ming Zhao & Liming Bai

  3. Graduate School of Science and Technology, Niigata University, Niigata, 950-2181, Japan

    Ryo Hasegawa

  4. Department of Chemistry and Chemical Engineering, Niigata University, Niigata, 950-2181, Japan

    Jun-ichi Sakai & Masayoshi Ando

  5. Niigata Research Laboratory, Mitsubishi Gas Chemical Company, Inc., Niigata, 950-3112, Japan

    Toshiaki Hasegawa

  6. Center for Biological Resources and Informatics, Tokyo Institute of Technology, Yokohama, 226-8501, Japan

    Tomokazu Mitsui, Hirotsugu Ogura & Takao Kataoka

  7. KNC Laboratories Co. Ltd., Kobe, 651-2271, Japan

    Katsutoshi Hirose

Authors
  1. Yuhua Bai
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  2. Ming Zhao
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  3. Liming Bai
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  4. Ryo Hasegawa
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  5. Jun-ichi Sakai
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  7. Tomokazu Mitsui
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  8. Hirotsugu Ogura
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  9. Takao Kataoka
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  10. Katsutoshi Hirose
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  11. Masayoshi Ando
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Correspondence to Yuhua Bai.

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Bai, Y., Zhao, M., Bai, L. et al. The biological activities of cardenolide triglycosides from stems, twigs, and leaves of Nerium oleander . J Wood Sci 57, 56–65 (2011). https://doi.org/10.1007/s10086-010-1132-3

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  • DOI: https://doi.org/10.1007/s10086-010-1132-3

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