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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Enlargement of individual cellulose microfibrils in transgenic poplars overexpressing xyloglucanase

Journal of Wood Science volume 57, pages 71–75 (2011)Cite this article

Abstract

Holocellulose samples prepared from transgenic poplars overexpressing xyloglucanase had crystal widths of 3.2–3.5 nm as a result of the (2 0 0) plane, based on their X-ray diffraction patterns, and crystal widths were greater than those of the wild type (3.0 nm). Cellulose microfibril widths in the holocellulose samples were further determined from transmission electron microscopic (TEM) images of individualized fibrils prepared by 2,2,6,6-tetramethylpiperidine-1-oxy radical-mediated oxidation of the holocelluloses and the successive disintegration of the oxidized products in water. The TEM images also supported the finding that cellulose microfibril widths of transgenic poplars were larger than those of the wild type. The cellulose microfibril widths of transgenic poplars were approximately 6 nm, whereas those of the wild type were about 5 nm. However, such enlargement of cellulose microfibril widths could not be explained by the increased cellulose contents of the transgenic poplars alone.

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Authors and Affiliations

  1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Mako Yamamoto, Tsuguyuki Saito & Akira Isogai

  2. Forest Bio-Research Center, Forestry and Forest Products Research Institute, Hitachi, Ibaraki, 319-1301, Japan

    Manabu Kurita

  3. Forest Tree Breeding Center, Forestry and Forest Products Research Institute, Hitachi, Ibaraki, 319-1301, Japan

    Teiji Kondo & Toru Taniguchi

  4. RISH & Institute of Sustainability Science, Kyoto University, Uji, 611-0011, Japan

    Rumi Kaida, Keiichi Baba & Takahisa Hayashi

Authors
  1. Mako Yamamoto
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  2. Tsuguyuki Saito
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  3. Akira Isogai
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  4. Manabu Kurita
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  5. Teiji Kondo
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  6. Toru Taniguchi
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  7. Rumi Kaida
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  8. Keiichi Baba
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  9. Takahisa Hayashi
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Correspondence to Akira Isogai.

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Yamamoto, M., Saito, T., Isogai, A. et al. Enlargement of individual cellulose microfibrils in transgenic poplars overexpressing xyloglucanase. J Wood Sci 57, 71–75 (2011). https://doi.org/10.1007/s10086-010-1140-3

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  • DOI: https://doi.org/10.1007/s10086-010-1140-3

Key words

  • Transgenic poplar
  • Xyloglucanase
  • Cellulose fibril
  • TEMPO-mediated oxidation