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Official Journal of the Japan Wood Research Society

Acetic acid fermentability with Clostridium thermoaceticum and Clostridium thermocellum of standard compounds found in beech wood as produced in hot-compressed water

Abstract

Acetic acid fermentability of various compounds from beech wood as produced in a two-step hot-compressed water treatment was evaluated by fermentation tests using standard compounds with Clostridium thermoaceticum and Clostridium thermocellum. For cellulose- and hemicellulose-derived products, the former microorganism was found to ferment compounds with low molecular weights such as monosaccharides, decomposed products, and organic acids to acetic acid, while the latter was found to ferment compounds with high molecular weights such as polysaccharides and oligosaccharides to acetic acid. Lignin-derived products were, on the other hand, fermented by both microorganisms to acetic acid. Based on these lines of evidence, co-culture with C. thermoaceticum and C. thermocellum was evaluated and proven to increase acetic acid fermentability. Consequently, almost all compounds produced from beech wood in hot-compressed water were found to be converted to acetic acid when using these microorganisms in combination. Thus, hot-compressed water treatment coupled with acetic acid fermentation would likely be a powerful method to produce acetic acid from lignocellulosics.

References

  1. Eggeman T, Verser D (2006) The importance of utility systems in today’s biorefineries and a vision for tomorrow. Appl Biochem Biotechnol 129–132:361–381

    Article  PubMed  Google Scholar 

  2. Lu X, Yamauchi K, Phaiboonsilpa N, Saka S (2009) Two-step hydrolysis of Japanese beech as treated by semi-flow hot-compressed water. J Wood Sci 55:367–375

    CAS  Article  Google Scholar 

  3. Fontaine FE, Peterson WH, McCoy E, Johnson MJ (1942) A new type of glucose fermentation by Clostridium thermoaceticum n. sp. J Bacteriol 43:701–715

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Andressen JR, Schaupp A, Neurauter C, Brown A, Ljungdahl LG (1973) Fermentation of glucose, fructose, and xylose by Clostridium thermoaceticum: Effect of metal on growth yield, enzymes, and the synthesis of acetate from CO2. J Bacteriol 114:743–751

    Google Scholar 

  5. Balasubramanian N, Kim JS, Lee YY (2001) Fermentation of xylose into acetic acid by Clostridium thermoaceticum. Appl Biochem Biotechnol 91–93:367–376

    Article  PubMed  Google Scholar 

  6. Ljungdahl LG (1986) The autotrophic pathway of acetate synthesis in acetogen bacteria. Ann Rev Microbiol 40:415–450

    CAS  Article  Google Scholar 

  7. Drake HL, Daniel SL (2004) Physiology of the thermophilic acetogen Moorella thermoacetica. Res Microbiol 155:422–436

    CAS  Article  PubMed  Google Scholar 

  8. Wu Z, Daniel SL, Drake HL (1988) Characterization of a CO-dependent O-demethylating enzyme system from the acetogen Clostridium thermoaceticum. J Bacteriol 170:5747–5750

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. Daniel SL, Wu Z, Drake HL (1988) Growth of thermophilic acetogenic bacteria on methoxylated aromatic acid. FEMS Microbiol Lett 52:25–28

    CAS  Article  Google Scholar 

  10. Hsu T, Daniel SL, Lux MF, Drake HL (1990) Biotransformation of carboxylated aromatic compounds by the acetogen Clostridium thermoaceticum: Generation of growth-supportive CO2 equivalents under CO2-limited condition. J Bacteriol 172:212–217

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  11. Lux MF, Keith ES, Hsu T, Drake HL (1990) Biotransformation of aromatic aldehydes by acetogenic bacteria. FEMS Microbiol Lett 67:73–78

    CAS  Article  Google Scholar 

  12. Daniel SL, Keith ES, Yang H, Lin YS, Drake HL (1991) Utilization of methoxylated aromatic compounds by the acetogen Clostridium thermoaceticum: Expression and specificity of the CO-dependent O-demethylating activity. Biochem Biophys Res Commun 180:416–422

    CAS  Article  PubMed  Google Scholar 

  13. Kasmi AE, Rajasekharan, Ragsdale SW (1994) Anaerobic pathway for conversion of the methyl group of aromatic methyl ethers to acetic acid by Clostridium thermoaceticum. Biochemistry 33:11217–11224

    CAS  Article  PubMed  Google Scholar 

  14. Hernandez PE (1982) Transport of d-glucose in Clostridium thermocellum ATCC-27405. J Gen Appl Microbiol 28:469–477

    CAS  Article  Google Scholar 

  15. Florenzano G, Poulain M, Goma G (1984) A study of acetate production from cellulose using Clostridium thermocellum. Biomass 4:295–303

    CAS  Article  Google Scholar 

  16. Rani KS, Swamy MV, Seenayya G (1997) Increased ethanol production by metabolic modulation of cellulose fermentation in Clostridium thermocellum. Biotechnol Lett 19:819–823

    CAS  Article  Google Scholar 

  17. Levin DB, Islam R, Cicek N, Sparling R (2006) Hydrogen production by Clostridium thermocellum 27405 from cellulosic biomass substrates. Int J Hydrog Energy 31:1496–1503

    CAS  Article  Google Scholar 

  18. Islam R, Cicek N, Sparling R, Levin D (2006) Effect of substrate loading on hydrogen production during anaerobic fermentation by Clostridium thermocellum 27405. Appl Microbiol Biotechnol 72:576–583

    CAS  Article  PubMed  Google Scholar 

  19. Islam R, Cicek N, Sparling R, Levin D (2009) Influence of initial cellulose concentration on the carbon flow distribution during batch fermentation by Clostridium thermocellum ATCC 27405. Appl Microbiol Biotechnol 82:141–148

    CAS  Article  PubMed  Google Scholar 

  20. Morag E, Bayer EA, Lamed R (1990) Relationship of cellulosomal and noncellulosomal xylanase of Clostridium thermocellum to cellulose-degrading enzyme. J Bacteriol 172:6098–6105

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  21. Wiegel J, Mothershed CP, Puls J (1985) Differences in xylan degradation by various noncellulolytic thermophilic anaerobes and Clostridium thermocellum. Appl Environ Microbiol 49:656–659

    CAS  PubMed  PubMed Central  Google Scholar 

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Correspondence to Shiro Saka.

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Nakamura, Y., Miyafuji, H., Kawamoto, H. et al. Acetic acid fermentability with Clostridium thermoaceticum and Clostridium thermocellum of standard compounds found in beech wood as produced in hot-compressed water. J Wood Sci 57, 331–337 (2011). https://doi.org/10.1007/s10086-010-1169-3

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  • DOI: https://doi.org/10.1007/s10086-010-1169-3

Key words

  • Acetic acid fermentation
  • Clostridium thermoaceticum
  • Clostridium thermocellum
  • Hot-compressed water
  • Beech wood