Skip to main content

Official Journal of the Japan Wood Research Society

Degradation of endosulfan and endosulfan sulfate by white-rot fungus Trametes hirsuta

Abstract

Endosulfan, an organochlorine insecticide, and its metabolite endosulfan sulfate are persistent in environments and are considered toxic. We investigate the possible nontoxic bioremediation of endosulfan. An endosulfandegrading fungus that does not produce endosulfan sulfate was selected from eight species of white-rot fungi. High degradation of endosulfan and low accumulation of endosulfan sulfate were found in cultures of Trametes hirsuta. A degradation experiment using endosulfan sulfate as the substrate revealed that T. hirsuta is able to further degrade endosulfan sulfate following the oxidative conversion of endosulfan to endosulfan sulfate. Endosulfan and endosulfan sulfate were converted to several metabolites via hydrolytic pathways. In addition, endosulfan dimethylene, previously reported as a metabolite of the soil bacterium Arthrobacter sp., was detected in T. hirsuta culture containing endosulfan sulfate. Our results suggest that T. hirsuta has multiple pathways for the degradation of endosulfan and endosulfan sulfate and thus has great potential for use as a biocatalyst in endosulfan bioremediation.

References

  1. Wan MT, Kuo JN, Buday C, Schroeder G, Van Aggelen G, Pasternak J (2005) Toxicity of α-, β-, (α + β)-endosulfan and their formulated and degradation products to Daphnia magna, Hyalella azteca, Oncorhynchus mykiss, Oncorhynchus kisutch, and biological implications in streams. Environ Toxicol Chem 24:1146–1154

    CAS  Article  PubMed  Google Scholar 

  2. U.S. EPA (2002) Environmental fate and ecological risk assessment for the re-registration eligibility decision on endosulfan (Thiodan). DP Barcode D238673. United States Environmental Protection Agency, Office of Pesticide Programs, Environmental Fate and Effects Division, Washington, DC

    Google Scholar 

  3. Berntssen MH, Glover CN, Robb DH, Jakobsen JV, Petri D (2008) Accumulation and elimination kinetics of dietary endosulfan in Atlantic salmon (Salmo salar). Aquat Toxicol 86:104–111

    CAS  Article  PubMed  Google Scholar 

  4. Dietrich D, Hickey WJ, Lamar R (1995) Degradation of 4,4′-dichlorobiphenyl, 3,3′,4,4′-tetrachlorobiphenyl, and 2,2′,4,4′,5,5′-hexachlorobiphenyl by the white rot fungus Phanerochaete chrysosporium. Appl Environ Microbiol 61:3904–3909

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Yadav JS, Quensen JF, Tiedje JM, Reddy CA (1995) Degradation of polychlorinated biphenyl mixtures (Aroclors 1242, 1254, and 1260) by the white rot fungus Phanerochaete chrysosporium as evidenced by congener-specific analysis. Appl Environ Microbiol 61:2560–2565

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Kamei I, Sonoki S, Haraguchi K, Kondo R (2006) Fungal bioconversion of toxic polychlorinated biphenyls by white-rot fungus Phlebia brevispora. Appl Microbiol Biotechnol 73:932–940

    CAS  Article  PubMed  Google Scholar 

  7. Bumpus JA, Tien M, Wright D, Aust SD (1985) Oxidation of persistent environmental pollutants by a white rot fungus. Science 228:1434–1436

    CAS  Article  PubMed  Google Scholar 

  8. Valli K, Wariishi H, Gold MH (1992) Degradation of 2,7-dichlorodibenzo-p-dioxin by the lignin-degrading basidiomycete Phanerochaete chrysosporium. J Bacteriol 174:2131–2137

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. Takada S, Nakamura M, Matsueda T, Kondo R, Sakai K (1996) Degradation of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans by the white rot fungus Phanerochaete sordida YK-624. Appl Environ Microbiol 62:4323–4328

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Kamei I, Suhara H, Kondo R (2005) Phylogenetical approach to isolation of white-rot fungi capable of degrading polychlorinated dibenzo-p-dioxin. Appl Microbiol Biotechnol 69:358–366

    CAS  Article  PubMed  Google Scholar 

  11. Kullman SW, Matsumura F (1996) Metabolic pathways utilized by Phanerochaete chrysosporium for degradation of the cyclodiene pesticide endosulfan. Appl Environ Microbiol 62:593–600

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Hussain S, Arshad M, Saleem M, Zahir ZA (2007) Screening of soil fungi for in vitro degradation of endosulfan. World J Microbiol Biotechnol 23:939–945

    CAS  Article  Google Scholar 

  13. Goswami S, Vig K, Singh DK (2009) Biodegradation of alpha and beta endosulfan by Aspergillus sydoni. Chemosphere 75:883–888

    CAS  Article  PubMed  Google Scholar 

  14. Kataoka R, Takagi K, Sakakibara F (2010) A new endosulfan-degrading fungus, Mortierella species, isolated from a soil contaminated with organochlorine pesticides. J Pest Sci 35:326–332

    CAS  Article  Google Scholar 

  15. Kamei I, Takagi K, Kondo R (2010) Bioconversion of dieldrin by wood-rotting fungi and metabolite detection. Pest Manag Sci 66:888–891

    CAS  PubMed  Google Scholar 

  16. Tien M, Kirk TK (1988) Lignin peroxidase of Phanerochaete chrysosporium. Method Enzymol 161B:238–249

    Article  Google Scholar 

  17. Martens R (1976) Degradation of [8,9,-14C]endosulfan by soil microorganisms. Appl Environ Microbiol 31:853–858

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Kwon GS, Sohn HY, Shin KS, Kim E, Seo BI (2005) Biodegradation of the organochlorine insecticide, endosulfan, and the toxic metabolite, endosulfan sulfate, by Klebsiella oxytoca KE-8. Appl Microbiol Biotechnol 67:845–850

    CAS  Article  PubMed  Google Scholar 

  19. Bajaj A, Pathak A, Mudiam MR, Mayilraj S, Manickam N (2010) Isolation and characterization of a Pseudomonas sp. strain IITR01 capable of degrading alpha-endosulfan and endosulfan sulfate. J Appl Microbiol doi: 10.1111/j.1365-2672.2010.04845.x

  20. Bhalerao TS, Puranik PR (2007) Biodegradation of organochlorine pesticide, endosulfan, by a fungal soil isolate, Aspergillus niger. Int Biodeterior Biodegrad 59:315–321

    CAS  Article  Google Scholar 

  21. Shetty PK, Mitra J, Murthy NBK, Namitha KK, Savitha KN, Raghu K (2000) Biodegradation of cyclodiene insecticide endosulfan by Mucor thermohyalospora MTCC 1384. Curr Sci 79:1381–1383

    CAS  Google Scholar 

  22. Sutherland TD, Weir KM, Lacey MJ, Horne I, Russell RJ, Oakeshott JG (2002) Enrichment of a microbial culture capable of degrading endosulphate, the toxic metabolite of endosulfan. J Appl Microbiol 92:541–548

    CAS  Article  PubMed  Google Scholar 

  23. Sutherland TD, Horne I, Russell RJ, Oakeshott JG (2002) Gene cloning and molecular characterization of a two-enzyme system catalyzing the oxidative detoxification of beta-endosulfan. Appl Environ Microbiol 68:6237–6245

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  24. Weir KM, Sutherland TD, Horne I, Russell RJ, Oakeshott JG (2006) A single monooxygenase, ese, is involved in the metabolism of the organochlorides endosulfan and endosulfate in an Arthrobacter sp. Appl Environ Microbiol 72:3524–3530

    CAS  Article  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ichiro Kamei.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kamei, I., Takagi, K. & Kondo, R. Degradation of endosulfan and endosulfan sulfate by white-rot fungus Trametes hirsuta . J Wood Sci 57, 317–322 (2011). https://doi.org/10.1007/s10086-011-1176-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10086-011-1176-z

Key words

  • Endosulfan
  • Endosulfan sulfate
  • Biodegradation
  • White-rot fungi