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Official Journal of the Japan Wood Research Society

Table 2 Experimental design and results of the Box-Behnken design of response surface methodology for the optimization of filter paper-degrading activity from T. reesei ATCC 66587

From: Ethanol production from sugi pulp under simultaneous saccharification and fermentation using a cocktail enzyme of T. reesei and A. tubingensis produced by solid-state fermentation

Run no.

(NH4)2SO4 (%)

Corn cob (%)

Culture period (days)

FP ase (U/ml)

Experimental

Predicted

1

−1

−1

0

2.21

2.03

2

1

−1

0

4.57

4.40

3

−1

1

0

4.41

3.73

4

1

1

0

5.09

6.09

5

−1

0

−1

2.02

2.70

6

1

0

−1

2.53

2.36

7

−1

0

1

1.85

2.00

8

1

0

1

7.75

7.06

9

0

−1

−1

4.26

4.15

10

0

1

−1

2.61

2.17

11

0

−1

1

2.05

2.47

12

0

1

1

7.75

7.84

13

0

0

0

8.42

8.57

14

0

0

0

9.35

8.57

15

0

0

0

7.97

8.57