Fig. 2
From: Coniferin β-glucosidase is ionically bound to cell wall in differentiating xylem of poplar
Phylogenetic analysis of coniferin BGL in angiosperm and gymnosperm. a Diagram of PtrBGL6 composed by sequence on database and putative additional sequences to begin with start codon (ATG) and end by stop codon. The putative additional sequences were expected by DNA sequence on database without considering additional intron. b Maximum-likelihood phylogeny of BGLs in Arabidopsis thaliana (At), Pinus contorta (Pc) and Populus trichocarpa (Ptr). AtBGL45, AtBGL46, PcBGL (hatched) have been identified as coniferin BGL [17, 18]. PtrBGL6 (bold) has similarity with identified coniferin BGLs. Bootstrapping was performed with 100 replicates. Bootstrap values are in gray type. c Alignment of amino acid sequences of PtrBGL6 and identified coniferin BGLs. All sequences were predicted to have signal peptide by SignalP (http://www.cbs.dtu.dk/services/SignalP/), which are shown in bold. Peptides of hatched sequence were used to raise anti-PtrBGL6 antibody. Underlined sequences indicate the putative additional sequences shown in a