Fig. 4
From: Coniferin β-glucosidase is ionically bound to cell wall in differentiating xylem of poplar
Glucosidase activities in the supernatant after immunoprecipitation with the anti-PtrBGL6 antibody. Glucosidase substrates were 10 mM p-nitrophenyl-β-glucoside (a, b) and 5 mM coniferin (c). Glucosidase activity was measured after immunoprecipitation without serum as control. The amounts of antibody indicated were used for immunoprecipitation in b and c. Following immunoprecipitation, supernatant fractions were incubated with substrate for 30 min. Fractions incubated without substrates served as background. Sol soluble protein fraction, WIB cell wall ionically bound protein fraction. Values are mean ± SD (n = 3 technical replicates)