Official Journal of the Japan Wood Research Society
Cell viability and melanogenesis activity (%) | Substrate | Tyrosinase activity (%) | |||||
---|---|---|---|---|---|---|---|
Extract concentrations | 200 μg/ml | 100 μg/ml | 50 μg/ml | 125 μg/ml | 63 μg/ml | 32 μg/ml | |
Cell viability | 70 ± 1.6* | 96 ± 1.6 | 97 ± 1.4 | l-Tyrosine | 37 ± 25.4 | 80 ± 9.0 | 84 ± 1.2 |
Intracellular melanin | 299 ± 15.3** | 220 ± 33.1* | 113 ± 1.8 | l-DOPA | 55 ± 10.1* | 81 ± 10.5 | 87 ± 6.3 |
Extracellular melanin | 139 ± 3.4 | 131 ± 4.5 | 116 ± 1.8 | ||||
1 Concentrations | 100 μM | 50 μM | 25 μM | 200 μM | 100 μM | 50 μM | |
Cell viability | 100 ± 7.6 | 98 ± 1.1 | 102 ± 1.5 | l-Tyrosine | 140 ± 5.8 | 113 ± 2.7 | 88 ± 0.2 |
Intracellular melanin | 72 ± 0.0* | 68 ± 0.8* | 73 ± 0.8* | l-DOPA | 101 ± 0.8 | 101 ± 2.3 | 84 ± 1.8 |
Extracellular melanin | 16 ± 0.5** | 13 ± 0.2** | 12 ± 0.2** | ||||
2 Concentrations | 92 μM | 46 μM | 23 μM | 200 μM | 100 μM | 50 μM | |
Cell viability | 45 ± 2.8** | 48 ± 1.9** | 73 ± 8.1* | l-Tyrosine | 84 ± 0.1 | 92 ± 1.1 | 74 ± 0.9 |
Intracellular melanin | 249 ± 27.4* | 106 ± 10.6 | 94 ± 6.3 | l-DOPA | 78 ± 0.3 | 89 ± 0.8 | 84 ± 0.1 |
Extracellular melanin | 142 ± 12.9 | 85 ± 0.0 | 79 ± 0.0 | ||||
3 Concentrations | 200 μM | 100 μM | 10 μM | 200 μM | 100 μM | 50 μM | |
Cell viability | 70 ± 0.7* | 95 ± 2.8 | 100 ± 3.3 | l-Tyrosine | 77 ± 1.9 | 90 ± 0.8 | 93 ± 0.6 |
Intracellular melanin | 75 ± 14.2 | 86 ± 1.7 | 100 ± 3.3 | l-DOPA | 80 ± 1.8 | 86 ± 2.1 | 91 ± 1.7 |
Extracellular melanin | 146 ± 0.5* | 125 ± 0.9 | 99 ± 1.5 | ||||
4 Concentrations | 200 μM | 100 μM | 10 μM | 200 μM | 100 μM | 50 μM | |
Cell viability | 85 ± 3.4 | 93 ± 0.2 | 99 ± 6.9 | l-Tyrosine | 50 ± 0.8* | 70 ± 1.1 | 79 ± 0.7 |
Intracellular melanin | 103 ± 6.9 | 111 ± 6.4 | 124 ± 3.7 | l-DOPA | 70 ± 0.6* | 88 ± 0.3 | 79 ± 1.3 |
Extracellular melanin | 121 ± 0.3 | 113 ± 2.9 | 107 ± 2.6 | ||||
5 Concentrations | 200 μM | 100 μM | 10 μM | 200 μM | 100 μM | 50 μM | |
Cell viability | 93 ± 3.7 | 93 ± 7.0 | 97 ± 1.3 | l-Tyrosine | 85 ± 1.6 | 76 ± 0.0 | 79 ± 1.3 |
Intracellular melanin | 148 ± 5.3* | 132 ± 0.0* | 106 ± 1.0 | l-DOPA | 98 ± 2.9 | 98 ± 1.7 | 104 ± 2.2 |
Extracellular melanin | 109 ± 2.6 | 109 ± 0.2 | 103 ± 2.3 | ||||
6 Concentrations | 200 μM | 100 μM | 10 μM | 200 μM | 100 μM | 50 μM | |
Cell viability | 172 ± 5.5** | 123 ± 6.1 | 90 ± 8.9 | l-Tyrosine | 86 ± 0.4 | 82 ± 1.2 | 83 ± 0.8 |
Intracellular melanin | 174 ± 13.6 | 140 ± 22.4 | 115 ± 4.4 | l-DOPA | 94 ± 1.5 | 95 ± 1.2 | 98 ± 0.1 |
Extracellular melanin | 132 ± 27.6 | 115 ± 24.1 | 112 ± 6.9 | ||||
Theophylline | 500 μM | 250 μM | 125 μM | ||||
Cell viability | 91.4 ± 1.3 | 94.4 ± 4.1 | 95.5 ± 11.0 | ||||
Intracellular melanin | 166.8 ± 31.7* | 131.7 ± 1.9 | 127.6 ± 6.6 | ||||
Extracellular melanin | 204 ± 1.6** | 183.2 ± 3.2** | 170.7 ± 0.7** | ||||
Arbutin | 730 μM | 365 μM | 183 μM | ||||
Cell viability | 97.9 ± 1.3 | 98.4 ± 1.1 | 88.3 ± 7.5 | ||||
Intracellular melanin | 81.2 ± 0.6 | 85.7 ± 0.6 | 96.0 ± 1.3 | ||||
Extracellular melanin | 26.1 ± 4.4** | 30.3 ± 0.4** | 44.5 ± 0.4** |