Screening for melanogenesis-controlled agents using Sudanese medicinal plants and identification of active compounds in the methanol extract of Terminalia brownii bark

Yamauchi, Kosei; Mitsunaga, Tohru; Muddathir, Ali Mahmoud

doi:10.1007/s10086-016-1546-7

Journal of Wood Science

Official Journal of the Japan Wood Research Society

Table 2 Tyrosinase activity, cell viability and intracellular and extracellular melanogenesis activity of Terminalia brownii bark MeOH extract and its isolated compounds [gallic acid (1), α,β-punicalagin (2), α,β-terchebulin (3), ellagic acid 4-O-α-l-rhamnopyranoside (4), ellagic acid (5), 3,4,3′-tri-O-methylellagic acid (6)] using B16 melanoma cells

From: Screening for melanogenesis-controlled agents using Sudanese medicinal plants and identification of active compounds in the methanol extract of Terminalia brownii bark

Cell viability and melanogenesis activity (%)				Substrate	Tyrosinase activity (%)
Extract concentrations	200 μg/ml	100 μg/ml	50 μg/ml		125 μg/ml	63 μg/ml	32 μg/ml
Cell viability	70 ± 1.6*	96 ± 1.6	97 ± 1.4	l-Tyrosine	37 ± 25.4	80 ± 9.0	84 ± 1.2
Intracellular melanin	299 ± 15.3**	220 ± 33.1*	113 ± 1.8	l-DOPA	55 ± 10.1*	81 ± 10.5	87 ± 6.3
Extracellular melanin	139 ± 3.4	131 ± 4.5	116 ± 1.8
1 Concentrations	100 μM	50 μM	25 μM		200 μM	100 μM	50 μM
Cell viability	100 ± 7.6	98 ± 1.1	102 ± 1.5	l-Tyrosine	140 ± 5.8	113 ± 2.7	88 ± 0.2
Intracellular melanin	72 ± 0.0*	68 ± 0.8*	73 ± 0.8*	l-DOPA	101 ± 0.8	101 ± 2.3	84 ± 1.8
Extracellular melanin	16 ± 0.5**	13 ± 0.2**	12 ± 0.2**
2 Concentrations	92 μM	46 μM	23 μM		200 μM	100 μM	50 μM
Cell viability	45 ± 2.8**	48 ± 1.9**	73 ± 8.1*	l-Tyrosine	84 ± 0.1	92 ± 1.1	74 ± 0.9
Intracellular melanin	249 ± 27.4*	106 ± 10.6	94 ± 6.3	l-DOPA	78 ± 0.3	89 ± 0.8	84 ± 0.1
Extracellular melanin	142 ± 12.9	85 ± 0.0	79 ± 0.0
3 Concentrations	200 μM	100 μM	10 μM		200 μM	100 μM	50 μM
Cell viability	70 ± 0.7*	95 ± 2.8	100 ± 3.3	l-Tyrosine	77 ± 1.9	90 ± 0.8	93 ± 0.6
Intracellular melanin	75 ± 14.2	86 ± 1.7	100 ± 3.3	l-DOPA	80 ± 1.8	86 ± 2.1	91 ± 1.7
Extracellular melanin	146 ± 0.5*	125 ± 0.9	99 ± 1.5
4 Concentrations	200 μM	100 μM	10 μM		200 μM	100 μM	50 μM
Cell viability	85 ± 3.4	93 ± 0.2	99 ± 6.9	l-Tyrosine	50 ± 0.8*	70 ± 1.1	79 ± 0.7
Intracellular melanin	103 ± 6.9	111 ± 6.4	124 ± 3.7	l-DOPA	70 ± 0.6*	88 ± 0.3	79 ± 1.3
Extracellular melanin	121 ± 0.3	113 ± 2.9	107 ± 2.6
5 Concentrations	200 μM	100 μM	10 μM		200 μM	100 μM	50 μM
Cell viability	93 ± 3.7	93 ± 7.0	97 ± 1.3	l-Tyrosine	85 ± 1.6	76 ± 0.0	79 ± 1.3
Intracellular melanin	148 ± 5.3*	132 ± 0.0*	106 ± 1.0	l-DOPA	98 ± 2.9	98 ± 1.7	104 ± 2.2
Extracellular melanin	109 ± 2.6	109 ± 0.2	103 ± 2.3
6 Concentrations	200 μM	100 μM	10 μM		200 μM	100 μM	50 μM
Cell viability	172 ± 5.5**	123 ± 6.1	90 ± 8.9	l-Tyrosine	86 ± 0.4	82 ± 1.2	83 ± 0.8
Intracellular melanin	174 ± 13.6	140 ± 22.4	115 ± 4.4	l-DOPA	94 ± 1.5	95 ± 1.2	98 ± 0.1
Extracellular melanin	132 ± 27.6	115 ± 24.1	112 ± 6.9
Theophylline	500 μM	250 μM	125 μM
Cell viability	91.4 ± 1.3	94.4 ± 4.1	95.5 ± 11.0
Intracellular melanin	166.8 ± 31.7*	131.7 ± 1.9	127.6 ± 6.6
Extracellular melanin	204 ± 1.6**	183.2 ± 3.2**	170.7 ± 0.7**
Arbutin	730 μM	365 μM	183 μM
Cell viability	97.9 ± 1.3	98.4 ± 1.1	88.3 ± 7.5
Intracellular melanin	81.2 ± 0.6	85.7 ± 0.6	96.0 ± 1.3
Extracellular melanin	26.1 ± 4.4**	30.3 ± 0.4**	44.5 ± 0.4**

Cells were treated with the extract or its components for 72 h following melanogenesis activity was measured. Cell viability was determined by the MTT method. Tyrosinase activity of the extract and 1–6 were determined using l-tyrosine and l-DOPA as substrate. The activities of melanine production are expressed as percentage to that of control (100 %). Theophylline and arbutin are used as positive control of melanogenic stimulator and inhibitor respectively
The data of theophylline are described in [20]
* p < 0.05 and ** p < 0.01 compared with respective control values n = 2

Back to article page