Table 1 Culture conditions employed for tobacco BY-2 and A. thaliana protoplasts

From: Secretion of a callose hollow fiber from herbaceous plant protoplasts induced by inhibition of cell wall formation

	Culture medium	Additive
Condition 1	LS, 0.4 M mannitol, pH 5.8	–
Condition 2	LS, 100 mM CaCl₂, pH 3.5	–
Condition 3	LS, 150 mM CaCl₂, pH 3.5	–
Condition 4	LS, 200 mM CaCl₂, pH 3.5	–
Condition 5	LS, 100 mM CaCl₂, pH 3.5	3 µM isoxaben
Condition 6	LS, 150 mM CaCl₂, pH 3.5	3 µM isoxaben
Condition 7	LS, 200 mM CaCl₂, pH 3.5	3 µM isoxaben

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