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Official Journal of the Japan Wood Research Society

Journal of Wood Science Cover Image

Table 1 Culture conditions employed for tobacco BY-2 and A. thaliana protoplasts

From: Secretion of a callose hollow fiber from herbaceous plant protoplasts induced by inhibition of cell wall formation

  Culture medium Additive
Condition 1 LS, 0.4 M mannitol, pH 5.8
Condition 2 LS, 100 mM CaCl2, pH 3.5
Condition 3 LS, 150 mM CaCl2, pH 3.5
Condition 4 LS, 200 mM CaCl2, pH 3.5
Condition 5 LS, 100 mM CaCl2, pH 3.5 3 µM isoxaben
Condition 6 LS, 150 mM CaCl2, pH 3.5 3 µM isoxaben
Condition 7 LS, 200 mM CaCl2, pH 3.5 3 µM isoxaben