Fig. 3

Two-dimensional electrophoresis (2DE) of the UB and AB cultures stained with Coomassie brilliant blue. a UB; b AB. P. chrysosporium was cultivated in synthetic medium containing 2.0% (w/v) UB or 2.0% (w/v) AB for 3 days. An aliquot (100 µg) of extracellular protein from each sample was separated as described in “Materials and methods”. The horizontal axis of the gel spans pH 4–7, and the vertical axis spans 250–10 kDa. Spot numbers correspond to the proteins in Table 1