Secretome analysis of the basidiomycete Phanerochaete chrysosporium grown on ammonia-treated lignocellulosic biomass from birch wood

Sakuragi, Kiyoshi; Hori, Chiaki; Igarashi, Kiyohiko; Samejima, Masahiro

doi:10.1007/s10086-018-1770-4

Journal of Wood Science

Official Journal of the Japan Wood Research Society

Table 1 Proteins secreted by P. chrysosporium after cultivation with untreated or ammonia-treated birch wood meal

From: Secretome analysis of the basidiomycete Phanerochaete chrysosporium grown on ammonia-treated lignocellulosic biomass from birch wood

Spot number	Score^a	Protein ID^b	Function (gene or domain)	Protein family	CBM^c	Th Mw^d	Th pI^d	Sequence coverage (%)
1	830	129849	Glucan β-glycosidase (gly3B)	GH 3	–	87.142	4.72	36
2	336	4361	Endo-β-1,4-glucanase (cel5B)	GH 5	N	49.730	6.21	17
3	308	5115	β-Mannanase (man5C)	GH 5	N	41.058	4.27	10
4	845	6458	Endo-β-1,4-glucanase (cel5A)	GH 5	N	39.856	5.04	49
5	989	133052	Cellobiohydrolase II (cel6A)	GH 6	N	48.967	5.04	41
6	343	133052	Cellobiohydrolase II (cel6A)	GH 6	N	48.967	5.04	30
7	299	137372	Cellobiohydrolase 58 (cel7D)	GH 7	C	59.497	4.96	25
8	416	137372	Cellobiohydrolase 58 (cel7D)	GH 7	C	59.497	4.96	34
9	2046	137372	Cellobiohydrolase 58 (cel7D)	GH 7	C	59.497	4.96	52
10	224	137372	Cellobiohydrolase 58 (cel7D)	GH 7	C	59.497	4.96	19
11	1326	127029	Cellobiohydrolase 62 (cel7C)	GH 7	C	56.128	5.03	54
12	2010	127029	Cellobiohydrolase 62 (cel7C)	GH 7	C	56.128	5.03	55
13	1448	127029	Cellobiohydrolase 62 (cel7C)	GH 7	C	56.128	5.03	50
14	683	127029	Cellobiohydrolase 62 (cel7C)	GH 7	C	56.128	5.03	45
15	1173	138345	Endo-β-1,4-xylanase (xyn10A)	GH 10	N	44.065	5.21	55
16	409	138345	Endo-β-1,4-xylanase (xyn10A)	GH 10	N	44.065	5.21	55
17	498	7045	Endo-β-1,4-xylanase (xyn10C)	GH 10	N^e	31.213	6.89	42
18	962	7045	Endo-β-1,4-xylanase (xyn10C)	GH 10	N^e	31.213	6.89	76
19	572	7045	Endo-β-1,4-xylanase (xyn10C)	GH 10	N^e	31.213	6.89	62
20	1033	7045	Endo-β-1,4-xylanase (xyn10C)	GH 10	N^e	31.213	6.89	76
21	537	7045	Endo-β-1,4-xylanase (xyn10C)	GH 10	N^e	31.213	6.89	75
22	428	133788	Endo-β-1,4-xylanase (xyn11B)	GH 11	C	30.811	5.72	21
23	114	133788	Endo-β-1,4-xylanase (xyn11B)	GH 11	C	30.811	5.72	17
24	184	7048	Putative endo-xyloglucanase (cel12B)	GH 12	–	27.084	4.74	10
25	179	8466	Endo-β-1,4-glucanase (cel12A)	GH 12	–	26.461	4.79	19
26	176	8466	Endo-β-1,4-glucanase (cel12A)	GH 12	–	26.461	4.79	8
27	277	29397	Putative rhamnogalacturonase (rgh28C)	GH 28	–	44.801	5.22	51
28	674	29397	Putative rhamnogalacturonase (rgh28C)	GH 28	–	44.801	5.22	50
29	91	4822	Endo-1,5-α-l-arabinanase	GH 43	–	33.101	4.82	9
30^f			Endo-β-1,4-glucanase (cel45)	GH 45	–	18.169	5.04
31	595	28013	Glycoside hydrolase family 74 (xgh74B)	GH 74	–	66.641	4.56	44
32	604	138226	Putative xyloglucanase (gly74A)	GH 74	–	77.792	4.72	36
33	973	134556	Xyloglucanase (xgh74B)	GH 74	C	89.860	5.05	43
34	1014	134556	Xyloglucanase (xgh74B)	GH 74	C	89.860	5.05	41
35	123	3280	Thaumatin-like protein (thn2)		–	27.768	4.74	14
36	97	3280	Thaumatin-like protein (thn2)		–	27.768	4.74	14
37	240	5297	Thaumatin-like protein (thn1)		–	29.288	4.99	14
38	143	5297	Thaumatin-like protein (thn1)		–	29.288	4.99	14
39	697	126075	Putative acetyl xylan esterase (axe1)	CE 1	–	35.970	5.91	29
40	511	126075	Putative acetyl xylan esterase (axe1)	CE 1	–	35.970	5.91	29
41	772	126075	Putative acetyl xylan esterase (axe1)	CE 1	–	35.970	5.91	29
42	391	126075	Putative acetyl xylan esterase (axe1)	CE 1	–	35.970	5.91	29
43	776	126075	Putative acetyl xylan esterase (axe1)	CE 1	–	35.970	5.91	29
44	275	129015	Carbohydrate esterase family 1 protein (axe1)	CE 1	N^e	30.753	6.20	21
45	289	129015	Carbohydrate esterase family 1 protein (axe1)	CE 1	N^e	30.753	6.20	13
46	439	132137	Pectin methylesterase	CE 8	–	36.287	5.84	36
47	554	130517	Putative glucuronoyl esterase	CE 15	N	49.741	5.55	28
48	245	3097	Fungal cellulose binding domain-containing protein	CE 16	N	43.216	5.08	15
49	821	11098	Iron reductase domain/cellobiose dehydrogenase (cdh)	AA8-AA3_1	–	82.189	5.19	31
50	313	41563	Lytic polysaccharide monooxygenase	AA9	C	33.203	5.39	29
51	301	121193	Lytic polysaccharide monooxygenase	AA9	C	32.562	4.31	25
52	207	138479	Aldose 1-epimerase (a1e1)		–	38.454	4.98	27
53	316	8221	Hypothetical protein (IPR010829 Cerato-platanin)		–	14.970	4.69	33
54	453	3085	Hypothetical protein		–	40.214	5.02	35
55	117	2416	Hypothetical protein		–	17.519	8.44	27

^aIn-house MASCOT score
^bProtein model number from the P. chrysosporium genome database version 2.0
^cDeduced amino acid sequence with and without a carbohydrate binding module (CBM); N N-terminal, C C-terminal
^dTheoretical molecular mass (Th Mw, kDa) and pI
^eManual annotation revealed existence of a signal peptide and/or a CBM sequences in the gene
^fMS/MS dataset of spot number 30 searched against genome version 2.0 in Scaffold 6:1798039–1798139 [29]

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