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Official Journal of the Japan Wood Research Society

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Table 1 Proteins secreted by P. chrysosporium after cultivation with untreated or ammonia-treated birch wood meal

From: Secretome analysis of the basidiomycete Phanerochaete chrysosporium grown on ammonia-treated lignocellulosic biomass from birch wood

Spot number Scorea Protein IDb Function (gene or domain) Protein family CBMc Th Mwd Th pId Sequence coverage (%)
1 830 129849 Glucan β-glycosidase (gly3B) GH 3 87.142 4.72 36
2 336 4361 Endo-β-1,4-glucanase (cel5B) GH 5 N 49.730 6.21 17
3 308 5115 β-Mannanase (man5C) GH 5 N 41.058 4.27 10
4 845 6458 Endo-β-1,4-glucanase (cel5A) GH 5 N 39.856 5.04 49
5 989 133052 Cellobiohydrolase II (cel6A) GH 6 N 48.967 5.04 41
6 343 133052 Cellobiohydrolase II (cel6A) GH 6 N 48.967 5.04 30
7 299 137372 Cellobiohydrolase 58 (cel7D) GH 7 C 59.497 4.96 25
8 416 137372 Cellobiohydrolase 58 (cel7D) GH 7 C 59.497 4.96 34
9 2046 137372 Cellobiohydrolase 58 (cel7D) GH 7 C 59.497 4.96 52
10 224 137372 Cellobiohydrolase 58 (cel7D) GH 7 C 59.497 4.96 19
11 1326 127029 Cellobiohydrolase 62 (cel7C) GH 7 C 56.128 5.03 54
12 2010 127029 Cellobiohydrolase 62 (cel7C) GH 7 C 56.128 5.03 55
13 1448 127029 Cellobiohydrolase 62 (cel7C) GH 7 C 56.128 5.03 50
14 683 127029 Cellobiohydrolase 62 (cel7C) GH 7 C 56.128 5.03 45
15 1173 138345 Endo-β-1,4-xylanase (xyn10A) GH 10 N 44.065 5.21 55
16 409 138345 Endo-β-1,4-xylanase (xyn10A) GH 10 N 44.065 5.21 55
17 498 7045 Endo-β-1,4-xylanase (xyn10C) GH 10 Ne 31.213 6.89 42
18 962 7045 Endo-β-1,4-xylanase (xyn10C) GH 10 Ne 31.213 6.89 76
19 572 7045 Endo-β-1,4-xylanase (xyn10C) GH 10 Ne 31.213 6.89 62
20 1033 7045 Endo-β-1,4-xylanase (xyn10C) GH 10 Ne 31.213 6.89 76
21 537 7045 Endo-β-1,4-xylanase (xyn10C) GH 10 Ne 31.213 6.89 75
22 428 133788 Endo-β-1,4-xylanase (xyn11B) GH 11 C 30.811 5.72 21
23 114 133788 Endo-β-1,4-xylanase (xyn11B) GH 11 C 30.811 5.72 17
24 184 7048 Putative endo-xyloglucanase (cel12B) GH 12 27.084 4.74 10
25 179 8466 Endo-β-1,4-glucanase (cel12A) GH 12 26.461 4.79 19
26 176 8466 Endo-β-1,4-glucanase (cel12A) GH 12 26.461 4.79 8
27 277 29397 Putative rhamnogalacturonase (rgh28C) GH 28 44.801 5.22 51
28 674 29397 Putative rhamnogalacturonase (rgh28C) GH 28 44.801 5.22 50
29 91 4822 Endo-1,5-α-l-arabinanase GH 43 33.101 4.82 9
30f    Endo-β-1,4-glucanase (cel45) GH 45 18.169 5.04  
31 595 28013 Glycoside hydrolase family 74 (xgh74B) GH 74 66.641 4.56 44
32 604 138226 Putative xyloglucanase (gly74A) GH 74 77.792 4.72 36
33 973 134556 Xyloglucanase (xgh74B) GH 74 C 89.860 5.05 43
34 1014 134556 Xyloglucanase (xgh74B) GH 74 C 89.860 5.05 41
35 123 3280 Thaumatin-like protein (thn2)   27.768 4.74 14
36 97 3280 Thaumatin-like protein (thn2)   27.768 4.74 14
37 240 5297 Thaumatin-like protein (thn1)   29.288 4.99 14
38 143 5297 Thaumatin-like protein (thn1)   29.288 4.99 14
39 697 126075 Putative acetyl xylan esterase (axe1) CE 1 35.970 5.91 29
40 511 126075 Putative acetyl xylan esterase (axe1) CE 1 35.970 5.91 29
41 772 126075 Putative acetyl xylan esterase (axe1) CE 1 35.970 5.91 29
42 391 126075 Putative acetyl xylan esterase (axe1) CE 1 35.970 5.91 29
43 776 126075 Putative acetyl xylan esterase (axe1) CE 1 35.970 5.91 29
44 275 129015 Carbohydrate esterase family 1 protein (axe1) CE 1 Ne 30.753 6.20 21
45 289 129015 Carbohydrate esterase family 1 protein (axe1) CE 1 Ne 30.753 6.20 13
46 439 132137 Pectin methylesterase CE 8 36.287 5.84 36
47 554 130517 Putative glucuronoyl esterase CE 15 N 49.741 5.55 28
48 245 3097 Fungal cellulose binding domain-containing protein CE 16 N 43.216 5.08 15
49 821 11098 Iron reductase domain/cellobiose dehydrogenase (cdh) AA8-AA3_1 82.189 5.19 31
50 313 41563 Lytic polysaccharide monooxygenase AA9 C 33.203 5.39 29
51 301 121193 Lytic polysaccharide monooxygenase AA9 C 32.562 4.31 25
52 207 138479 Aldose 1-epimerase (a1e1)   38.454 4.98 27
53 316 8221 Hypothetical protein (IPR010829 Cerato-platanin)   14.970 4.69 33
54 453 3085 Hypothetical protein   40.214 5.02 35
55 117 2416 Hypothetical protein   17.519 8.44 27
  1. aIn-house MASCOT score
  2. bProtein model number from the P. chrysosporium genome database version 2.0
  3. cDeduced amino acid sequence with and without a carbohydrate binding module (CBM); N N-terminal, C C-terminal
  4. dTheoretical molecular mass (Th Mw, kDa) and pI
  5. eManual annotation revealed existence of a signal peptide and/or a CBM sequences in the gene
  6. fMS/MS dataset of spot number 30 searched against genome version 2.0 in Scaffold 6:1798039–1798139 [29]