Secretome analysis of the basidiomycete Phanerochaete chrysosporium grown on ammonia-treated lignocellulosic biomass from birch wood

Journal of Wood Science

Official Journal of the Japan Wood Research Society

Table 2 Increased 2DE spot volumes between ammonia-treated and untreated birch wood meal cultures

Spot number	Protein family	Function (gene or domain)	Normalized average volume		Relative degree
Spot number	Protein family	Function (gene or domain)	UB × 10⁷	AB × 10⁷	Relative degree
36		Thaumatin-like protein	0.13	0.64	5.0
22	GH 11	Endo-β-1,4-xylanase (xyn11B)	0.60	2.9	4.9
19	GH 10	Putative endo-β-1,4-xylanase (xyn10C)	0.25	1.2	4.7
55		Hypothetical protein	0.07	0.28	4.2
23	GH 11	Endo-β-1,4-xylanase (xyn11B)	0.12	0.52	4.2
52	A1e1	Aldose 1-epimerase (a1e1)	0.24	0.83	3.4
38		Thaumatin-like protein	0.09	0.27	3.0
14	GH 7	Cellobiohydrolase 62 (cel7C) (Fragment)	0.30	0.70	2.4
24	GH 12	Putative endoxyloglucanase (cel12B)	0.43	1.0	2.4
13	GH 7	Cellobiohydrolase 62 (cel7C) (Fragment)	1.6	3.6	2.2

Spots showing a normalized average volume increase by twofold or more on 2DE gels in AB vs. UB cultures. Spot numbers correspond to those in Fig. 3 and Table 1, and the intensity was analyzed by Prodigy 2D ver. 1.0. The relative degree calculation equals the normalized average volume of AB divided by the normalized average volume of UB
UB untreated birch wood meal, AB ammonia-treated birch wood meal