Secretome analysis of the basidiomycete Phanerochaete chrysosporium grown on ammonia-treated lignocellulosic biomass from birch wood

Journal of Wood Science

Official Journal of the Japan Wood Research Society

Table 3 Decreased 2DE gel spot volumes in ammonia-treated vs. untreated birch wood meal cultures

Spot number	Protein family	Function (gene or domain)	Normalized average volume		Relative degree
Spot number	Protein family	Function (gene or domain)	UB × 10⁷	AB × 10⁷	Relative degree
33	GH 74	Xyloglucanase (xgh74B)	0.45	0.04	0.087
20	GH 10	Putative endo-β-1,4-xylanase (xyn10C)	0.92	0.12	0.14
34	GH 74	Xyloglucanase (xgh74B)	0.81	0.17	0.21
27	GH 28	Putative rhamnogalacturonase (rgh28C)	0.14	0.04	0.30
49	AA8-AA3_1	Iron reductase domain/cellobiose dehydrogenase (cdh)	0.56	0.20	0.36
39	CE 1	Putative acetyl xylan esterase (axe1)	0.71	0.32	0.45

Spots showing a normalized average volume decreased by 0.5-fold or greater on 2DE gels in AB vs. UB cultures. Spot numbers correspond to those in Fig. 3 and Table 1, and the intensity was analyzed by Prodigy 2D version 1.0. The relative degree was calculated as the normalized average volume of AB divided by the normalized average volume of UB
UB untreated birch wood meal, AB ammonia-treated birch wood meal