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Cell growth and nutrient uptake by cell suspensions of Cupressus lusitanica
Journal of Wood Science volume 49, pages 0005–0010 (2003)
Abstract
Conditions for cell growth of suspension cultures of Cupressus lusitanica, which has high β-thujaplicin productivity, were studied. The medium that provided the highest growth rate was IS-1 medium (pH 5.5), modified from Gamborg B5 medium containing 32 mM of total nitrogen. Its NO3-N/NH4-N ratio was 30 : 2. The maximum growth represented a 25-fold increase over the initial biomass on a fresh weight basis after 30 days of culture in this medium. The highest cell growth was obtained with an initial pH of 3.5–5.5, but the pH of the medium settled to about pH 4.0 from any of the initial pH values in this report. The cells cultured under this condition were able to produce a high level of β-thujaplicin.
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Received: September 7, 2001 / Accepted: May 7, 2002
Present address: Teijin Ltd., Ehime 791-8530, Japan
Present address: Q'SAI Co. Ltd., Fukuoka 811-3422, Japan
Part of this report was presented at the 10th international symposium on wood and pulp chemistry, Yokohama, Japan, June 1999
Correspondence to:K. Fujita
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Yamada, J., Fujita, K., Eto, K. et al. Cell growth and nutrient uptake by cell suspensions of Cupressus lusitanica . J Wood Sci 49, 0005–0010 (2003). https://doi.org/10.1007/s100860300001
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DOI: https://doi.org/10.1007/s100860300001