Official Journal of the Japan Wood Research Society
Table 1 Chemical composition of transgenic rice cell walls
| Â | Lignin, % (fold change vs untreated sample)a | Cellulose, % (fold change vs untreated sample)b | Hemicelluloses, % (fold change vs untreated sample)c |
|---|---|---|---|
Untreated | |||
 WT | 15.4 ± 1.4 (–) | 41.3 ± 0.7 (–) | 42.4 ± 1.7 (–) |
 OsCAld5H1-KD | 22.1 ± 1.1 (–) | 40.2 ± 0.7 (–) | 37.0 ± 4.5 (–) |
 OsCAld5H1-OX | 16.5 ± 0.9 (–) | 37.9 ± 0.5 (–) | 38.9 ± 1.3 (–) |
 OsC3´H1-KD | 18.7 ± 1.7 (–) | 44.0 ± 1.7 (–) | 31.7 ± 0.6 (–) |
Dilute alkali (31.25 mM NaOH, 90 °C, 3 h) | |||
 WT | 14.8 ± 0.3 (0.96) | 46.6 ± 1.8 (1.13) | 33.1 ± 1.7 (0.78) |
 OsCAld5H1-KD | 17.4 ± 1.7 (0.79) | 47.4 ± 0.2 (1.18) | 33.4 ± 1.6 (0.90) |
 OsCAld5H1-OX | 12.0 ± 0.8 (0.73) | 46.6 ± 0.5 (1.23) | 35.0 ± 6.6 (0.90) |
 OsC3´H1-KD | 14.5 ± 2.7 (0.78) | 48.8 ± 1.7 (1.11) | 25.3 ± 0.7 (0.80) |
Dilute acid (1.3% H2SO4, 90 °C, 3 h) | |||
 WT | 49.0 ± 0.6 (3.17) | 54.9 ± 1.0 (1.33) | 12.7 ± 0.1 (0.30) |
 OsCAld5H1-KD | 51.1 ± 1.3 (2.31) | 56.1 ± 1.2 (1.40) | 9.9 ± 1.1 (0.27) |
 OsCAld5H1-OX | 44.1 ± 0.9 (2.68) | 55.3 ± 1.2 (1.46) | 9.6 ± 0.6 (0.25) |
 OsC3´H1-KD | 27.9 ± 0.5 (1.50) | 53.8 ± 1.9 (1.22) | 9.8 ± 0.1 (0.31) |
Liquid hot water (200 °C, 10 min) | |||
 WT | 51.6 ± 3.9 (3.34) | 61.9 ± 2.1 (1.50) | 4.8 ± 0.4 (0.11) |
 OsCAld5H1-KD | 56.7 ± 0.9 (2.56) | 61.4 ± 4.0 (1.53) | 3.9 ± 0.2 (0.11) |
 OsCAld5H1-OX | 59.4 ± 3.9 (3.61) | 64.1 ± 3.8 (1.69) | 4.1 ± 0.2 (0.11) |
 OsC3´H1-KD | 60.4 ± 4.4 (3.24) | 65.9 ± 1.2 (1.50) | 4.8 ± 0.4 (0.15) |