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Official Journal of the Japan Wood Research Society

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Table 1 Chemical composition of transgenic rice cell walls

From: Comparative evaluations of lignocellulose reactivity and usability in transgenic rice plants with altered lignin composition

  Lignin, % (fold change vs untreated sample)a Cellulose, % (fold change vs untreated sample)b Hemicelluloses, % (fold change vs untreated sample)c
Untreated
 WT 15.4 ± 1.4 (–) 41.3 ± 0.7 (–) 42.4 ± 1.7 (–)
 OsCAld5H1-KD 22.1 ± 1.1 (–) 40.2 ± 0.7 (–) 37.0 ± 4.5 (–)
 OsCAld5H1-OX 16.5 ± 0.9 (–) 37.9 ± 0.5 (–) 38.9 ± 1.3 (–)
 OsC3´H1-KD 18.7 ± 1.7 (–) 44.0 ± 1.7 (–) 31.7 ± 0.6 (–)
Dilute alkali (31.25 mM NaOH, 90 °C, 3 h)
 WT 14.8 ± 0.3 (0.96) 46.6 ± 1.8 (1.13) 33.1 ± 1.7 (0.78)
 OsCAld5H1-KD 17.4 ± 1.7 (0.79) 47.4 ± 0.2 (1.18) 33.4 ± 1.6 (0.90)
 OsCAld5H1-OX 12.0 ± 0.8 (0.73) 46.6 ± 0.5 (1.23) 35.0 ± 6.6 (0.90)
 OsC3´H1-KD 14.5 ± 2.7 (0.78) 48.8 ± 1.7 (1.11) 25.3 ± 0.7 (0.80)
Dilute acid (1.3% H2SO4, 90 °C, 3 h)
 WT 49.0 ± 0.6 (3.17) 54.9 ± 1.0 (1.33) 12.7 ± 0.1 (0.30)
 OsCAld5H1-KD 51.1 ± 1.3 (2.31) 56.1 ± 1.2 (1.40) 9.9 ± 1.1 (0.27)
 OsCAld5H1-OX 44.1 ± 0.9 (2.68) 55.3 ± 1.2 (1.46) 9.6 ± 0.6 (0.25)
 OsC3´H1-KD 27.9 ± 0.5 (1.50) 53.8 ± 1.9 (1.22) 9.8 ± 0.1 (0.31)
Liquid hot water (200 °C, 10 min)
 WT 51.6 ± 3.9 (3.34) 61.9 ± 2.1 (1.50) 4.8 ± 0.4 (0.11)
 OsCAld5H1-KD 56.7 ± 0.9 (2.56) 61.4 ± 4.0 (1.53) 3.9 ± 0.2 (0.11)
 OsCAld5H1-OX 59.4 ± 3.9 (3.61) 64.1 ± 3.8 (1.69) 4.1 ± 0.2 (0.11)
 OsC3´H1-KD 60.4 ± 4.4 (3.24) 65.9 ± 1.2 (1.50) 4.8 ± 0.4 (0.15)
  1. WT, wild-type; OsCAld5H1-KD, OsCAld5H1-knockdown; OsCAld5H1-OX, OsCAld5H1-overexpression; OsC3´H1-KD, OsC3´H1-knockdown
  2. aSum of the Klason acid-insoluble (corrected by ash content) and acid-soluble lignin contents
  3. bTrifluoroacetic acid (TFA)-insoluble glucan content
  4. cSum of TFA-soluble arabinan, xylan, mannan, galactan and glucan contents. Values are mean ± SD (n = 3)