Fig. 4
From: Selective fluorescence labeling: time-lapse enzyme visualization during sugarcane hydrolysis
A time-lapse flow-diagram observation of enzyme localization in a sugarcane section. A stack of image slices was registered and carefully aligned (a). Intensity at each pixel point in the stack as a function of time. Up to 300 profiles randomly extracted from 250,000 profiles in total were exemplified in b. The eight representative profiles obtained by k-means clustering (c) together with a typical contour map of the corresponding eight regions (d)