Fig. 1

Characterizations of optimal pH, temperature, and stability of recombinant GtCel12A. a The optimal pH and pH stability were measured at 50 °C with 50 mM buffers at a pH range of pH 2.0–9.0 on CMC; pH stability was determined by monitoring residual activity after preincubation without substrate for 18 h at 4 °C. b Optimal temperature was determined at various temperatures (20–80 °C) in 50 mM glycin–HCl (pH 2.5). c Thermal stability was determined at 50 °C in 50 mM glycin–HCl (pH 2.5) by the preincubated enzyme in 30–60 °C for various time (0, 10, 30, 60, 90, and 120 min). All of error bars indicate ± SD