Official Journal of the Japan Wood Research Society
Table 1 Comparison of optimal conditions and specific activity of GtCel12A to those of other endoglucanases
Source | Protein | GH family | Host | Specific activity (U mg−1) | Optimal condition | References | ||||
|---|---|---|---|---|---|---|---|---|---|---|
β-Glucan | CMC | Xyloglucan | Filter paper | Temperature (°C) | pH | |||||
Gloeophyllum trabeum | Cel12A | 12 | P. pastoris | 6546 ± 33.5 | 1129 ± 16.3 | 10.1 ± 0.03 | 0.21 ± 0.001 | 50 | 2.5 | This study |
Cel5B | 5 | P. pastoris | 3675 ± 198.2 | 483 ± 12.0 | – | 1.004 ± 0.04 | 55 | 3.5 | [25] | |
Trichoderma reesei | EGIII | 12 | * | – | 20 | 17 | – | – | 5.7 | [31] |
E. coli | 60 | 15 | 64 | – | – | 6.0 | [15] | |||
EGII | 5 | P. pastoris | – | 220.2 | – | ND | 55 | 5.0 | [32] | |
Penicillium oxalicum | XEG12A | 12 | P. pastoris | ND | ND | 172 | – | 55–60 | 4.5–5.5 | [18] |
Aspergillus terreus | GH12 | 12 | A. nidulans | 256 ± 0.11 | 3.33 ± 0.04 | 115 ± 0.04 | – | 55 | 5.0 | [17] |