Official Journal of the Japan Wood Research Society
Table 1 Primers used in this study
From: Genetic analyses of causal genes of albinism (white fruiting body) in Grifola frondosa
Primer | Sequence | Use |
|---|---|---|
TYR2gF | 5′-TCTTCATCCTGCTTCCTCTATC-3′ | Amplification of full-length genomic clone of tyr2 |
TYR2gR | 5′-GCTTGCACCATCGAGACAGCCAA-3′ | |
Btyr2F | 5′-TCATCACTCATTTCCCTGCTGACAC-3′ | Used for detection of wild tyr2 gene (tyr2+) |
Btyr2R | 5′-CCCGTAACGATTTCGCCACTCTC-3′ | |
Wtyr2F | 5′-CATTTTCCTGCTGACACCTT-3′ | Used for detection of mutant tyr2 gene (tyr2−Δ25) |
Wtyr2R | 5′-CGACGG TGATAATCCAGTCA-3′ | |
3RTYR2 | 5′-AGGCGGGCATATGGCTACTG-3′ | Used for 3′-RACE |
5RTYR2P | 5′-(P)CAGATCACTTGCTCC-3′ | Used for 5′-RACE (5′-end of this oligonucleotide was phosphorylated) |
5RTYR2S1 | 5′-GCTATTGTACTCACGGAACTGTC-3′ | Used for 5′-RACE |
5RTYR2A1 | 5′-AGTCCCCAATATTGACATCATTCC-3′ | |
5RTYR2S2 | 5′-CTCACGGAACTGTCCTCTTC-3′ | |
5RTYR2A2 | 5′-TTCCAAGGAGTGTATGGCAA-3′ |