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Journal of Wood Science

Official Journal of the Japan Wood Research Society

Fig. 1 | Journal of Wood Science

Fig. 1

From: Marker-free genome editing in the edible mushroom, Pleurotus ostreatus, using transient expression of genes required for CRISPR/Cas9 and for selection

Fig. 1

Transient hph expression in Pleurotus ostreatus. a Schematic diagram for transient hph expression. b Plasmid map of pPHT1. CctubB_pro or ter: promoter or terminator from tubulin gene in Coprinopsis cinerea, ColE1 ori: origin for Escherichia coli, KmR: kanamycin resistance gene, AmpR: ampicillin resistance gene, hph: hygromycin phosphotransferase. c PCR amplification of hph fragment using primer set hph_F and hph_R. Lane M, 1-kb ladder (Nippon gene); lane P, pPHT1 (positive control); lane W, parental strain PC9 (negative control); lanes 1–5, PHT1-1–5 strains. HygR: hygromycin B resistant, HygS: hygromycin B sensitive

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