Marker-free genome editing in the edible mushroom, Pleurotus ostreatus, using transient expression of genes required for CRISPR/Cas9 and for selection

Journal of Wood Science

Official Journal of the Japan Wood Research Society

Table 1 Pleurotus ostreatus strains used in this study

Strain	Genotype/description	Source
PC9	A2B1	[20]
PHT1-1	A2B1 Hyg^Ra strain obtained after introducing pPHT1	This study
PHT1-2	A2B1 Hyg^R strain obtained after introducing pPHT1	This study
PHT1-3	A2B1 Hyg^Sb strain obtained after introducing pPHT1	This study
PHT1-4	A2B1 Hyg^S strain obtained after introducing pPHT1	This study
PHT1-5	A2B1 Hyg^S strain obtained after introducing pPHT1	This study
Pef3-1	A2B1 5-FC^Rc Hyg^R strain obtained after introducing pCcPef3-126-fcy1sg2	This study
Pef3-2	A2B1 5-FC^R Hyg^R strain obtained after introducing pCcPef3-126-fcy1sg2	This study
Pef3-3	A2B1 5-FC^R Hyg^S strain obtained after introducing pCcPef3-126-fcy1sg2	This study
Pef3-4	A2B1 5-FC^R Hyg^S strain obtained after introducing pCcPef3-126-fcy1sg2	This study
Pef3-5	A2B1 5-FC^R Hyg^S strain obtained after introducing pCcPef3-126-fcy1sg2	This study
Pef3-6	A2B1 5-FC^R Hyg^S strain obtained after introducing pCcPef3-126-fcy1sg2	This study