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Official Journal of the Japan Wood Research Society

Table 3 Results of the transient transformation using hph-based plasmid

From: Marker-free genome editing in the edible mushroom, Pleurotus ostreatus, using transient expression of genes required for CRISPR/Cas9 and for selection

Plasmid

Number of strains

5-FC resistant

Hyg sensitive

Loss of hph

pPHT1

46

N/A

3

3

pCcpef3-126-fcy1sg2

131

130

4

1