Fig. 5

Light and fluorescence micrographs showing transverse views of cambium and differentiating xylem cells after 30 days of application of high frequency watering on 25 April 2015. A newly differentiating vessel located close to the cambium consisted of large radial diameter and thin cell wall (a). The vessel located close to the cambium exhibited a narrow diameter and thicker cell walls than the surrounding wood fiber cells (b). Small vessels located close to the cambium exhibited thick cell walls (c, d). Fluorescence micrographs showing the phloem, cambium and differentiating (arrows) and differentiated vessel elements located close to the cambium (e, f). The heterogeneous intensity of fluorescence in (e, f) indicates the less-lignified rays and wood fibers adjacent to cambium. Merged green (Ex/Em, BP450–490/BP500–550) and red (Ex/Em, BP539–563/BP570–640) image. Ap axial parenchyma, C cambium, * Ph, phloem, *, vessel elements, Xy xylem. Bars = 50 µm