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Official Journal of the Japan Wood Research Society

Fig. 5 | Journal of Wood Science

Fig. 5

From: The Populus alba cationic cell-wall-bound peroxidase (CWPO-C) regulates plant growth, lignin content and composition in poplar

Fig. 5

Expression of CWPO-C and CWPO-C protein in transgenic poplar lines. a, b CWPO-C expression levels in 6-week-old overexpression (OE) and suppression (RNAi) transgenic poplar lines. a CWPO-C expression in WT and three OE lines. b CWPO-C expression in WT and two RNAi lines. Relative expression levels (mean ± standard deviation) measured by RT-qPCR were calculated from three biological replicates. Data are presented as relative transcript abundance normalized to UBQ expression. c, d Western blot analysis of CWPO-C protein in transgenic lines using an anti-CWPO-C antibody. c WT and three OE lines. d WT and two RNAi lines. Upper panels: recombinant CWPO-C protein as a standard marker (rCWPO-C, arrow). Lower panels: proteins stained with Coomassie brilliant blue; arrow indicates Rubisco. L: molecular weight markers

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