Fig. 2

Primer sequences and their positions. A Primer sequences used for mutagenesis and vector construction. Red lower-case letters represent substituted nucleotides. The flanking sequences of the HindIII recognition sites in pBIC1–4 are underlined. Yellow-highlighted sequences are HindIII, EcoRI or BamHI recognition sites. Primer A and D: primers for full length prxA3a amplification. Primer B and C: primers for F77Y mutagenesis. Primer E and F: primers for A165W mutagenesis. G and H: Primers for creation of DNA fragments for B. chosinensis transformation. Primer G contains a BamHI site and causes an amino acid residue substitution of Ser to Asp at the third position of the native mature PrxA3a. B Positions of the primers for mutagenesis and vector construction on prxA3a.