Fig. 6

In vitro analysis of coniferyl alcohol polymerization abilities of peroxidases by size exclusion chromatography. UV chromatograms at 278 nm are shown. Closed triangles, open triangles, closed diamonds, open diamonds, double parentheses, parentheses, and arrows represent monomer, dimer, trimer, tetramer, oligomer (13–18 min), polymer (9–13 min), and coniferyl aldehyde, respectively. CA coniferyl alcohol, NC non-enzyme control; WT, FY, and FYAW: reactants of wild-type, FY-mutated, and FYAW-mutated enzymes are shown at the top. AEach enzyme and coniferyl alcohol were allowed to react for 1 h. B Each enzyme and coniferyl alcohol were allowed to react for 24 h. The area ratios of the polymer bands after 24 h of reaction were 25.4, 40.4, and 42.4% for the wild-type, FY-mutated, and FYAW-mutated enzymes, respectively