Heterologous production and secretion of active-form plant peroxidase in Brevibacillus choshinensis and their monolignol polymerization abilities analysis

Journal of Wood Science

Official Journal of the Japan Wood Research Society

Table 1 The activity and concentration of wild-type and mutated PrxA3a were measured

	(i) Enzyme activity	(ii) 1 mL equivalent of enzyme activity [U/mL]	(iii) Protein quantification	(iv) Protein concentration [mg/mL]	(v) Specific activity [U/mg]
Wild type (Sample volume)	0.275 ± 0.0155 (5 µL)	55.0	0.293 ± 0.0273 (10 µL)	0.574	95.8
FY mutanted (Sample volume)	0.454 ± 0.0218 (2 µL)	227.0	0.468 ± 0.0188 (4 µL)	2.24	101
FYAW mutanted (Sample volume)	0.260 ± 0.0145 (5 µL)	52.0	0.228 ± 0.0160 (10 µL)	0.455	114

(i)The guaiacol oxidizing activities of wild-type and mutated PrxA3a were measured. (ii) Enzyme activity per 1 mL was calculated based on the measured guaiacol oxidation activity. (iii) and (iv) Protein concentrations were determined based on the Bradford method. (v) Specific activity was calculated using enzyme activity per 1 mL and protein concentration. The SD were calculated from the triplicate experiments.