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Official Journal of the Japan Wood Research Society

Table 1 Advantages and disadvantages of the enzymatic and sulfuric acid methods for CNF quantification

From: Applicability of enzymatic and phenol–sulfuric acid methods for determination of cellulose nanofibers in ecotoxicity testing

 

Enzymatic method

Phenol–sulfuric acid method

Lower limit of measurable concentration

~ 1 mg/L

~ 10 mg/L

Time required for measurement

~ 24 h

~ 2 h

Required reagents

Cellulase, glucose assay kit (somewhat costly)

Phenol, sulfuric acid (deleterious substances)

Interference by algae

Low

High

Interference by non-cellulosic polysaccharides

Low

High