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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Screening of extracts of Japanese woods for melanin biosynthesis inhibition

Journal of Wood Science volume 53, pages 153–160 (2007)Cite this article

Abstract

The effects of 120 methanol extracts prepared from bark and heartwood of 69 types of Japanese wood on the melanin production of B16 melanoma cells were examined. The melanin content of B16 melanoma cells was determined spectrophotometrically at 405nm. The extracts were also examined for their effects on cell viability. We found that the methanol extracts of Fagus crenata (buna, wood, 100μg/ml), Sapium sebiferum (Nankinhaze, wood, bark, 10μg/ml), and Zelkova serrata (keyaki, wood, 10μg/ml) greatly inhibited the melanin production of B16 melanoma cells without significant cytotoxicity. However, these extracts did not inhibit tyrosinase activity at the concentration of 100μg/ml. These findings indicate that the depigmenting mechanism of these extracts involves the suppression of some pigmenting signals in stimulating melanogenesis rather than the inhibition of tyrosinase activity.

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Authors and Affiliations

  1. Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Kuniyoshi Shimizu, Shoko Fukunaga, Keisuke Yoshikawa & Ryuichiro Kondo

Authors
  1. Kuniyoshi Shimizu
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  2. Shoko Fukunaga
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  3. Keisuke Yoshikawa
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  4. Ryuichiro Kondo
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Correspondence to Ryuichiro Kondo.

Additional information

Part of this study was presented at the 53rd Annual Meeting of the Japan Wood Research Society, Fukuoka, Japan, March 2003

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Shimizu, K., Fukunaga, S., Yoshikawa, K. et al. Screening of extracts of Japanese woods for melanin biosynthesis inhibition. J Wood Sci 53, 153–160 (2007). https://doi.org/10.1007/s10086-006-0824-1

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  • DOI: https://doi.org/10.1007/s10086-006-0824-1

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