Table 1 Kinetic parameters of LeHex20B. The activity was measured in 20 mM sodium acetate buffer (pH 4.2) at 37 °C for 15 min. To determine the kinetic properties of LeHex20B, the reactions were performed with 0.05–0.5 mM of substrate

Substrate

K _m (μM)

k _cat (s⁻¹)

k _cat/K _m (s⁻¹ mM⁻¹)