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Official Journal of the Japan Wood Research Society

  • Original Article
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Antifungal and antitermitic activities of wood vinegar from oil palm trunk


We evaluated the antifungal and antitermite activities of wood vinegars produced from oil palm trunk. The wood vinegars were produced at three different pyrolysis temperatures, 350, 400, and 450 °C. Antifungal activities of vinegars were evaluated using a Petri dish bioassay with 0.5, 1.0, and 1.5% (v/v) against a white-rot fungus, Trametes versicolor, and a brown-rot fungus, Fomitopsis palustris. Antitermite activities were tested using a no-choice bioassay method for Coptotermes formosanus with 2.5, 5.0, 7.5, and 10.0% (v/v). All the wood vinegars exhibited antifungal activities against T. versicolor. In particular, the wood vinegar produced at 350 °C resulted in complete inhibition of T. versicolor growth at 1.0 and 1.5%. However, higher concentrations were required to obtain growth inhibition of F. palustris. All the wood vinegars exhibited antitermite activity to C. formosanus workers in the no-choice experiment at relatively high concentrations. For instance, 10% concentration was required to achieve 100% mortality against C. formosanus at all production temperatures. The lowest mass loss of the treated filter paper of 11.75% was obtained with a 350 °C—10.0% combination.


Wood and wood products are susceptible to biological degradation by fungi and termites. Synthetic chemicals have long been used to protect wood and wood products [1,2,3]; however, the problems associated with their use include the development of resistance by many pests, and the adverse negative effect to human health and the environment [4, 5]. In addition, synthetic chemicals are produced from unrenewable materials. Therefore, the development of alternative bio-renewable and abundant materials is needed to find alternative wood preservative. One such alternative is wood vinegar.

Wood vinegars prepared from various sources of wood and wood-based composites have been tested for their effectiveness against decay fungi and insects [5,6,7,8,9,10]. For example, vinegars obtained from wood-based composites have been shown to have inhibitory effects against decay fungi [8]. Bedtmutha et al. [5] reported the insecticidal activity of wood vinegars produced at 400–600 °C against Colorado potato beetle (Leptinotarsa decemlineata Say). Hossain et al. [10] tested the insecticidal activity of wood vinegars derived from fast pyrolysis of lignin, cellulose, and hemicellulose at 450 and 550 °C, and found that they were toxic at both temperatures against L. decemlineata, Trichoplusia ni, and Acyrthosiphon pisum.

Recently, Theapparat et al. [3] reported that wood vinegars exhibited antifungal activity against two white-rot fungi, a brown-rot fungus and a sapstain fungus. The vinegars were made from Eucalyptus camaldulensis, Leucaena leucocephala, Azadirachta indica, Hevea brasiliensis (rubberwood), and Dendrocalamus asper (bamboo). The largest component in the five wood vinegars that exhibited high fungicidal activities, especially in those made from rubberwood and bamboo, was total phenol. A previous study suggested that phenolic compounds derived from lignocellulosic biomass might be primarily responsible for the antifungal performance of wood vinegars [11]. Tar oil from macadamia nut shells was employed in vitro to protect wood against various wood-decaying fungi and wood-attacking termites [12].

Meanwhile, wood vinegar made from Cerbera odollam Gaertn produced at 300, 400, and 500 °C exhibited antitermite activity to Coptotermes formosanus in vitro [13]. Temiz et al. [14] investigated the termiticidal activity of a wood vinegar made from giant cane (Arundo donax L.), and found abundant components including acids, ketones, furans, benzenes, phenols, sugars, and guaiacols. They reported that the wood vinegar was effective against Reticulitermes flavipes. Shiny and Remadewi [15] reported that wood vinegar from shell oil had the potential to replace synthetic chemicals as a promising new biodegradable termiticide.

To the best of our knowledge, however, the antifungal and antitermite activities of wood vinegars made from oil palm trunk have not yet been reported. Oil palm plantations are spreading rapidly throughout Indonesia, resulting in substantial residues (i.e., felled oil palm trunk), because oil palm trees must be replaced at 25 year intervals [16]. In other words, oil palm trunk is an important agricultural wastes in Southeast Asia, and can be obtained at any of the plantation areas in Malaysia and Indonesia [17].

In this study, three wood vinegars obtained from oil palm trunk were examined for their antifungal activity against a brown-rot fungus, Fomitopsis palustris and a white-rot fungus, Trametes versicolor. The efficacy of the wood vinegars against an economically important termite, C. formosanus, was also evaluated.

Materials and methods

Preparation and pyrolysis of oil palm trunk

An oil palm trunk was collected at Bumi Pratama Khatulistiwa (BPK) Ltd. Mempawah Regency, West Kalimantan, Indonesia, and was converted into wood meal (20 meshpass) with a disk mill (Model FFC-23, Qingdao Dahua Double Circle Macheney, China). The wood meal was then air-dried to approximately 12% moisture content, and dried meal was pyrolyzed in a laboratory furnace, following Tranggono et al. [18] at pyrolysis temperature of 350, 400, and 450 °C, respectively. The each pyrolysis temperature was repeated with three replicates. Smoke emitted from the reactor was channeled into a cooling column through the pipeline, and cold water was then flowed into the column [18]. This pyrolysis process was conducted in the Laboratory of Agriculture Engineering, Agriculture Faculty, Gadjah Mada University, Yogyakarta, Indonesia.

Identification of wood vinegar by GC–MS

Components in the three wood vinegars were analyzed with a gas chromatography–mass spectrometry (GC–MS) system (QP-210S, Shimadzu Manufacturing, Kyoto, Japan). The analysis was conducted under the following conditions: Stabilwax-DA Columns (fused silica) capillary columns, 30 m × 0.25 mm; injection temperature: 225 °C; column temperature program: 50–225 °C increasing at 10°C/min; and He flow rate: 88.3 mL/min. The GC–MS was operated in the electron ionization mode at 70 eV with an interface temperature of 225 °C. The 1 µL wood vinegar was injected into the column. The chemicals were identified by comparing the MS spectra with the standard library data [19]. The chemical compositions of wood vinegar were determined by the integrated peak areas.

Determination of total phenol

Total phenol content of wood vinegars was estimated by a colorimetric assay, using the method described by Senter et al. [20]. An aliquot of wood vinegar (1 mL) was diluted 1000 times with deionized water (DI), and 1 mL of the diluted sample was added to 5 ml 2% Na2CO3 solution. After 10 min, 0.5 mL of Folin–Ciocalteau suspension was added to the solution. The absorbance of the solution at 750 nm was measured using a UV–Vis spectrophotometer (Shimadzu 1601, Shimadzu Manufacturing, Kyoto, Japan). The total phenol content of each wood vinegar was calculated in comparison with that of pure phenol suspension. Total phenol analyses were repeated with three replicates.

Determination of acid

The acidity of the wood vinegar was analyzed using the AOAC method [21]. 1 mL of the wood vinegar was diluted to 100 mL with DI, and then, three drops of the phenolphthalein indicator were added. The solution was titrated by 0.1 N NaOH. Three replicated runs were performed for acid determination.

Fungal inhibition bioassay

The fungal inhibition bioassay was performed according to Kartal et al. [22] and Kartal et al. [12]. Seven-day cultures of a brown-rot fungus, F. palustris (Berk. et Curt.) Gilb. & Ryv. and a white-rot fungus, T. versicolor (L. ex Fr.) Quel. were grown on potato dextrose agar (PDA) plates at 27 °C and used as inoculates. PDA media with 0.5, 1.0, and 1.5% (v/v) wood vinegars were autoclaved for 15 min at 121 °C and 103.4 kPa (15 psi), and then poured into Petri dishes (90 mm diameter). There was no significance change of components of the wood vinegars during sterilization in autoclave. After cooling, the Petri dishes were centrally inoculated with a single plug (5 mm diameter) from the pre-cultured PDA plates. Uninoculated PDA dishes were used as controls. Three replicates were conducted for each condition. The treated and control dishes were incubated in a conditioning room at 27 °C until the growth of fungi in controls reached the edge of the dishes. The colony diameter was measured daily, and the percentage mycelia inhibition rate was calculated by the following equation:

$$I={\text{ }}\left[ {\left( {C-T} \right)/{\text{C}}} \right]{\text{1}}00$$

where I = inhibition, as a percentage; C = colony diameter of mycelium from control Petri dishes (mm); and T = colony diameter of mycelium from the Petri dishes containing the wood vinegars (mm).

Antitermite test

Mature workers and soldiers of C. formosanus were obtained from a laboratory colony at the Deterioration Organisms Laboratory (DOL), Research Institute for Sustainable Humanosphere (RISH), Kyoto University. The no-choice bioassay method was performed described by Kang et al. [23] and Ganapaty et al. [24]. DI-diluted wood vinegars (0.3 ml) with concentrations of 0, 2.5, 5.0, 7.5, and 10.0% (v/v) were pipetted onto filter papers (Whatman No. 1, 55 mm diameter). The treated filter paper was placed into a Petri dish (60 mm in diameter), and 50 workers and 5 soldiers were placed on each filter paper. Filter papers treated with DI were used as controls. Test dishes were then covered and placed in an incubator maintained at 26.5 °C and 80% RH in the dark. Three replicates were made for each concentration, and the mortalities of the termites were counted daily for 21 days. The mortality of worker and soldier of termites was count and we did not separately count the mortality of each worker and each soldier due to fasten on observations to avoid on disturb the termites activity.

Statistical analysis

A 3 × 4 and 3 × 5 factorial completely randomized design were used for antifungal and antitermite activities, respectively. The first factor was pyrolysis temperature: 350, 400, or 450 °C for both antifungal and antitermite activities. The second factor was concentration: 0, 0.5, 1.0, and 1.5% (v/v) for antifungal activity, and 0, 2.5, 5.0, 7.5, and 10.0% (v/v) for antitermite activity. The means were separated using Duncan’s multiple range test at p = 0.05 for antifungal and antitermite activities, whereas for total phenol and acid, the means were separated using Tukey’s test at p = 0.05. All data were analyzed using the SAS software (version 8.2, SAS Institute Inc., NC, USA).

Results and discussion

Wood vinegar properties

The relative percentages of compounds identified by GC–MS in the wood vinegars at pyrolysis temperatures of 350, 400, and 450 °C are summarized in Table 1. The major components of wood vinegar were achieved on different retention times due to difference of pyrolysis temperatures. The temperature of pyrolysis affected the degradation of wood chemical compound and resulted different amounts of each compound in wood vinegar. Similar to this result Theapparat et al. [3] stated the temperature of pyrolysis on wood made different retention times on GC–MS analysis. Overall, predominant compounds in wood vinegars were 1-hydroxy-propanone, 3-hydroxy-2-butanone, acetic acid, propanoic acid, and phenol. This compound has the higher peak on the graphic of GC–MS analysis. All of the compounds were found in wood vinegar with pyrolysis time 350 °C; meanwhile, in wood vinegar with pyrolysis time 400 and 450 °C, only three compounds found, namely, 1-hydroxy-2-propanone, acetic acid, and phenol. These five compounds were bioactive to inhibit the growth of fungi. Their relative percentage quantities in wood vinegar were dominant due to that they have higher peak on GC–MS analysis. A previous study reported that major compounds detected by GC–MS analyses of wood vinegars were organic acid and phenolic compounds such as phenol, cresol, guaiacol, eugenol, and syringol [25].

Table 1 Relative percentage quantities of compounds identified by GC–MS in the wood vinegars with different pyrolysis temperatures

Table 2 lists the total phenol and total acid content in the wood vinegars. Total phenol and total acid contents ranged from 2.88 to 3.22% and 3.63 to 4.38%, respectively. As shown in Table 2, total phenol in the wood vinegar prepared at 350 °C was significantly higher than those prepared at 400 and 450 °C. Similar results were found in the previous studies, where the total phenol was the largest component in wood vinegar from walnut shell and pineapple plant waste biomass [26, 27]. Choi et al. [28] found that the main phenolic compounds were simple phenols, such as phenol, cresols, and 1,2-benzenediol, which were derived from the thermal decomposition of lignin. Ma et al. [26] and Wu et al. [29] previously reported the detailed chemical analysis of wood vinegar. Wu et al. [29] analyzed wood vinegars made from three agricultural and forestry residue samples, Chinese fir sawdust, cotton stalk, and bamboo sawdust, and found that the main compounds were acids, phenols, and ketones. Mathew et al. [27] identified 48 compounds by GC–MS analysis of wood vinegar. In that study, phenols (69.5%), alkyl arylethers (9.33%), and ketones (7.76%) were the major constituents, while furan and pyran derivatives (3.57%), sugar derivatives (2.85%), organic acids (2.67%), esters (1.81%), aldehydes (1.05%), alcohols (0.9%), and nitrogenous compounds (0.14%) were minor constituents.

Table 2 Total phenol and acid concentrations in wood vinegars

Wu et al. [29] indicated that acetic and propionic acid were the major compounds in the acids, with acetic acid being dominant. Total acid content decreased as temperature was raised from 350 to 450 °C. Similarly, in the case of pyrolysis products of palm kernel shells [28], it was indicated that the acids in wood vinegars actively participated in the condensation reaction at elevated temperatures. They reported composition of wood vinegar obtained under different reaction temperatures (473–555 °C). The major acid compounds produced by cellulose and hemicellulose degradations were acetic, hydroxyl-benzoic, and dodecanoic acid. Heo et al. [30] also found that chemical compounds such as acetic and benzoic acids were influenced by temperature. These compounds rapidly increased with increasing temperature up to 500 °C. In addition, when the temperature was increased, the acid content in wood vinegar decreased from 28.0 to 18.6 wt %, while the water content increased from 34.2 to 48.0 wt % [28].

The chemical content of wood vinegar is affected by temperature and various other factors, including material size and proximate analysis (amount of cellulose, hemicellulose, lignin, volatile extractives, fixed carbon, and ash) in the samples [31, 32]. However, for the sake of practicality, we focused only on temperature in this research.

Growth inhibition performance against decay fungi

Table 3 shows percentage growth inhibition of the wood vinegars against decay fungi at concentrations from 0.5 to 1.5% (v/v). All the wood vinegars showed antifungal activity, and performance increased with concentration in both fungi. The wood vinegar at 350 °C showed the highest performance with 100% inhibition against the white-rot fungus, T. versicolor at 1.0 and 1.5%.

Table 3 Growth inhibition performance of wood vinegars from oil palm trunk against Trametes versicolor and Fomitopsis palustris on the PDA media

The reason why the highest antifungal activity of wood vinegar against T. versicolor was produced at 350 °C might be the higher amount of total phenol and total acid (Table 2) together with the characteristic existence of 3-hydroxy-2-butanone and propanoic acid (Table 1). The antifungal performance of the wood vinegars was significantly higher in the white-rot fungus, T. versicolor than in the brown-rot fungus, F. palustris. Even at the highest concentration (1.5%) of wood vinegar at 350 °C, F. palustris grew a bit (87% inhibition). Similar results were reported by Theapparat et al. [3] and by Oramahi and Yoshimura [9].

Kose and Kartal [33] and Arango et al. [34] stated that some brown-rot fungi including F. palustris secrete oxalic acid and have the higher resistance against Cu-based wood preservatives. In addition, white-rot fungi are more sensitive to natural chemicals extracted from white spruce (Picea glauca), jack pine (Pinus banksiana), and red pine (Pinus resinosa) cones such as pinosylvin, pinosylvin monomethyl ether, and pinosylvin dimethyl ether, and the extractives inhibited the growth of white-rot fungi (T. versicolor and Phanerochaete chrysosporium), but slightly stimulated the growth of brown-rot fungi (Neolentinus lepideus, Gloeophyllum trabeum, and Postia placenta) at the 1:1:1 mixture of these compounds [35]. The results of this work showed that the color around the fungal colony changed during the bioassay with F. palustris. It thus was assumed that lignin and phenolic compounds in wood vinegars were metabolized by F. palustris. Pandey and Nagveni [36] stated that the rate of degradation of brown-rot fungi is higher than white-rot fungi. The fungi T. versicolor has preference for lignin degradation, and resulted in the reduction on ratio of lignin/carbohydrate.

Theapparat et al. [3] examined the antifungal activity of wood vinegar from H. brasiliensis and D. asper against two white-rot fungi (T. versicolor and Rigidoporopsis amylospora) and a brown-rot fungus (G. trabeum), and found that both were effective as wood preservatives. Oramahi and Yoshimura [9] reported that wood vinegar from Vitex pubescens Vahl inhibited white-rot fungus, T. versicolor, and a brown-rot fungus, F. palustris. The phenol content of wood vinegar is also likely responsible for antifungal activity. Okutucu et al. [37] suggested the fungicidal activity of the phenolic content of wood vinegar from pistachio shell. Similarly, Kartal et al. [22] reported that phenolic compounds in wood vinegars could contribute to decay resistance and that among the wood vinegars made from acacia (Acacia mangium) at a pyrolysis temperature of 300 °C, and from sugi (Cryptomeria japonica) at pyrolysis temperatures of 270 and 300 °C, only the sugi wood vinegar at 270 °C showed growth inhibition against the white-rot fungus, T. versicolor, at 0.1% concentration. Shiny et al. [38] speculated that high phenolic content of wood vinegar from coconut shell might be the reason for its antifungal activity. These results are in good agreement with our findings in the present study.

The wood vinegar in PDA medium has effective to inhibit the growth of F. palustris and T. versicolor. This might be due to inhibition of enzymatic activity by the compounds of the wood vinegars. Martinez et al. [39] stated that white-rot fungi have extracellular oxidative enzymes (oxidoreductases) and involved in degradation on cell wall component. Machuca and Ferraz [40] reported that the brown-rot fungi and white-rot fungi produced hydrolytic activities and this enzyme has a main function on degradation of the chemical components in wood. These enzymes on hydrolytic activities consist of xylanase and β-glucosidase. White-rot fungi were faster on decay process and need 30–60 days, while brown-rot fungi need 120–150 days. Pandey and Nagveni [36] reported that degradation of wood structure by white-rot fungi caused both lignin and carbohydrate decrease; meanwhile, on wood attacked by brown-rot fungi, only the carbohydrate was reduced.

Antitermite performance

Mean percentage mass loss of the filter papers and mortalities of workers of C. formosanus are presented in Table 4. The mortalities were significantly increased with concentrations from 2.5 to 10% after 7, 14, and 21 days, and the filter papers treated with 10% solutions showed the highest mortalities in all the wood vinegars. The mass losses of filter papers after 21 days generally decreased with concentrations.

Table 4 Termiticidal performance of wood vinegars from oil palm trunk against Coptotermes formosanus in a no-choice test with treated filter papers

Termiticidal performance was consistent with concentration of total acid in the wood vinegars (Table 2), showing good agreement with the previous results. For example, Shiny and Remadewi [15] investigated the termiticidal activity of coconut shell oil against Odontotermes horni (Wasmann), O. obesus (Rambur), O. redamanni (Wasmann), and Microtermes obesi (Holmgren). They reported that the largest components in the coconut shell oil that exhibited high termiticidal performance were phenol or its highly oxygenated fractions. Temiz et al. [14] examined the termiticidal activity of wood vinegars, and stated that no mass losses were observed in treated samples, while untreated control samples showed 20% mass loss.

Sunarta et al. [41] concluded that wood vinegars made from oil palm fruit had the potential to be a low-cost and environmentally low-impact wood preservative for preventing attacks by Cryptotermes spp. Recently, wood vinegars from pineapple waste biomass were shown to have termiticidal activities against subterranean termites [42].


The wood vinegar from oil palm trunk produced at 350 °C completely inhibited the growth of the white-rot fungus, T. versicolor at 1.0 and 1.5% concentrations, whereas higher concentrations were required for the brown-rot fungus, F. palustris. The total phenol content of the vinegar produced at 350 °C was significantly higher than those of wood vinegars produced at 400 and 450 °C. The wood vinegar produced at 350 °C has the potential to prevent attacks by C. formosanus. The practical evaluation of these wood vinegars as wood preservatives is worthy of consideration.


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The authors would like to thank the Directorate General of Higher Education of Indonesia for financially supporting this project in fiscal year 2013–2014, through a competitive research grant. We thank the Laboratory of Innovative Humano-habitability, RISH, Kyoto University, Japan, for providing experimental materials and facilities for this research.

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Correspondence to Hasan Ashari Oramahi.

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Oramahi, H.A., Yoshimura, T., Diba, F. et al. Antifungal and antitermitic activities of wood vinegar from oil palm trunk. J Wood Sci 64, 311–317 (2018).

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