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Official Journal of the Japan Wood Research Society

  • Original Article
  • Published:

Natural durability of the culturally and historically important timber: Erythrophleum fordii wood against white-rot fungi

Abstract

The natural resistance of Erythrophleum fordii Oliver wood to degradation by Phanerochaete sordida and Phanerochaete chrysosporium white-rot fungi was investigated. In this study, Fagus crenata Blume (Japanese beech) was selected as reference species. The results showed that both fungi caused less than 2% mass loss in E. fordii wood, while the degradation of beech wood produced by P. chrysosporium and P. sordida was approximately 12 and 14%, respectively. Microscopic observations revealed high structural rigidity of E. fordii timber. Hyphae were only observed in the lumen of vessels and parenchymal cells, while the fibers were not affected. The E. fordii wood fiber consisted of highly lignified thick-walled fibers with the fiber lumina almost completely closed. Two-dimensional heteronuclear single-quantum coherence nuclear magnetic resonance evaluation revealed the E. fordii wood to have a highly condensed-lignin structure that reflected by the durability classes. These unique parameters are likely to be critical for the high natural resistance of E. fordii.

Introduction

Erythrophleum fordii Oliver is a precious hardwood tree species of Caesalpiniaceae and naturally distributed in Vietnam and South China. It is known by the name “Gemu” and is used by native Chinese people as an agent promoting invigoration and circulation [1, 2]. Moreover, Ganoderma lucidum (Curtis) P. Karst, which has high medicinal value, can be found in natural forests and plantations of this species when stumps decay. E. fordii has important medicinal value and toxic properties [3]. Alkaloids, triterpenoids, diterpenoids, and diterpenoid dimers have been isolated and identified from seeds, bark, and leaves [1, 2, 4,5,6,7]. The biological effects of alkaloids present in E. fordii have been reported [1, 5,6,7]. E. fordii also has important health and ecological benefits [8].

E. fordii is indeed a valuable timber tree species in tropical and subtropical regions with a clear distinction between heartwood and sapwood. The E. fordii tree produces quite hard, heavy, and durable wood, commonly called as ‘iron wood’, which is generally used for the production of ships, high-grade furniture, flooring, sculpture, and crafts [9, 10]. Because of its superior wood, the E. fordii was classified in the most durable wood group in Vietnam [11]. It has been used as the traditional timber in many historical buildings in Vietnam.

Recently, wooden artifacts excavated from archaeological sites in Vietnam, such as Thang Long imperial citadel, Hanoi, Bach Dang stake yard, Quang Ninh, were identified as E. fordii. Interestingly, despite those artifacts being buried for hundreds of years, the degree of degradation of E. fordii wood was limited. The degradation of the wood’s surface layer was limited to a depth of approximately 1–2 cm. Below the outer surface layer, microscopic observation, chemical analyses, and mechanical tests revealed no significant differences between excavated and modern wood [12]. Considering the medicinal and antifungal properties of different parts of the plant, it is suggested that E. fordii wood would exhibit these properties. However, there are limited reports on natural durability of E. fordii wood.

Various organisms can induce wood to deteriorate, and the greatest level of deterioration is caused by fungi. White-rot fungi are among the most efficient degraders of plant fiber (lignocellulose), and are capable of degrading cellulose, hemicellulose, and lignin. They commonly cause rotten wood to feel moist, soft, spongy, or stringy, and to appear white or yellow [13,14,15]. Wood undergoes a number of changes during the decay process, including reductions in mass and strength [16,17,18]. Significant changes occur in the chemical composition of the cell wall during the fungal attack [16]. Attack of fungi causes a decrease in mechanical and physical properties of wood, influencing its moisture content, electrical conduction, acoustics, elasticity, and plasticity [19, 20].

The degradation of wood by white-rot fungi has been reported [21,22,23]. Different methods have been applied to investigate wood decay, including microscopy techniques [24,25,26]; differential scanning calorimetric [27]; X-ray diffraction [28]; gas chromatography—mass spectrometry (GC–MS) spectroscopy, chemical analysis [29, 30]; Nuclear magnetic resonance (NMR); and Fourier transform infrared spectroscopy [31,32,33]. Two-dimensional (2D) NMR techniques in the cell wall and lignin research have improved over the past decade [34]. Among various 2D NMR spectroscopic techniques available, Heteronuclear Single-Quantum Coherence (HSQC) is the most common. Solution-state 2D NMR provided an interpretable structural fingerprint of the lignin and carbohydrates of the cell wall, without further structural modification applied during the ball milling and ultra-sonication step [33, 35].

In this study, microscopic observations and chemical analyses were performed to illustrate the structural and chemical changes of the E. fordii wood degraded by white-rot fungi Phanerochaete chrysosporium Burdsall and Phanerochaete sordida (P. Karst.) J. Erikss. & Ryvarden. The deterioration of E. fordii wood will be discussed and compared with Fagus crenata Blume (Japanese beech) wood. Investigation of natural resistance of E. fordii wood to wood decay fungi is essential for better understanding the characteristics of this wood, and for determining appropriate procedures to conserve archaeological waterlogged E. fordii wood.

Materials and methods

Materials

Samples of E. fordii and F. crenata Blume (Japanese beech) wood, 20 × 20 × 5 mm (tangential × radial × longitudinal dimensions), were cut from defect-free heartwood parts and used for further testing. The E. fordii wood was obtained from woods collection of Vietnamese Academy of Forest Sciences, Vietnam, while beech wood was received from Xylarium, RISH, Kyoto University, Japan.

Sulfuric acid was purchased from Wako Pure Chemical Industries, Ltd., Japan. Dimethyl sulfoxide-d6 (DMSO-d6) was obtained from Sigma-Aldrich, USA.

Wood decay testing

Wood samples were exposed to two white-rot fungi P. sordida ATCC 90872 and P. chrysosporium ATCC 34541 for 4 weeks. The wood samples were oven-dried at 103 °C for 24 h and weighed prior to fungal exposure. The 3.8% potato dextrose agar (PDA) aqueous solution was steam-sterilized at 120 °C for 20 min. In the next step, about 20 ml of PDA medium was poured into a 90 mm Petri dish. Fungi were cultivated in Petri dishes on PDA medium. After inoculation, Petri dishes were held at 28 °C and 70% relative humidity to enable the fungi to spread over the entire dish. The specimens (n = 3 for each fungus) were steam-sterilized under the same conditions and then placed on the medium. After 4 weeks of incubation, the mycelia covering the blocks were removed carefully, and the blocks were oven-dried to constant mass. The mass loss (W) of individual samples was calculated from the oven-dried mass before and after fungal test, and used to calculate mean percentage of mass losses:

$$\% W=\left[ {\left( {{W_0} - {W_{\text{f}}}} \right)/{W_0}} \right] \times {\text{1}}00,$$

where W0 is oven dry mass of sample prior to exposure and Wf is the oven dry mass following exposure to fungus.

Light and scanning electron microscopy observation

Optical microscopy

Small wood blocks, 2 × 2 × 2 mm (tangential × radial × longitudinal dimensions), were prepared from the nondegraded and biodegraded wood specimens. The specimens were dehydrated in a series of increasing concentration of acetone baths and embedded in Spurr resin [36]. The embedded specimens were cut at approximately 1 µm thickness with a semi-thin microtome (Leica, Solms, Germany) equipped with a diamond knife. The sections were stained with toluidine blue for 3 min and then washed with distilled water for 1–3 min. The sections were observed using an optical microscope (BX51; Olympus, Tokyo, Japan) to investigate patterns of hyphal and decay of wood tissue.

Scanning electron microscopy observation

The nondegraded and biodegraded specimens, 3 × 3 × 2 mm (tangential × radial × longitudinal dimensions), were prepared from internal part of samples. The clear wood surfaces were prepared using a microtome (TU-213, Yamato Scientific Co., Ltd., Japan). The specimens were freeze-dried for 2 days and then coated with platinum using an auto fine coater (JFC-1600, JEOL, Japan) operated at 30 mA for 90 s. Field-emission scanning electron microscopy (SEM, JSM-7800F prime, JEOL, Japan) was operated at an accelerating voltage of 1.5 kV.

Chemical analysis

Lignin content

Klason lignin content was determined in samples before fungal exposure. The nondegraded wood was powdered in a coffee grinder. The measurement was carried out on sieved material (in the range 60–100 mesh, corresponding to 0.15–0.25 mm). The lignin content was determined in triplicate following the TAPPI method [37], which is based on the isolation of Klason lignin after the hydrolysis of the polysaccharides (cellulose and hemicellulose). The wood powder was immersed in concentrated sulfuric acid (72%) for 4 h. In the next step, the solution was transferred to an Erlenmeyer flask and diluted to 3% acid concentration with distilled water. These samples were boiled for 4 h. The acid-insoluble lignin was then filtered off, oven-dried, and weighed.

2D HSQC NMR analysis

To provide more detailed structural information, 2D HSQC NMR experiments were performed on nondegraded and degraded samples. The selected samples were grinded using laboratory-scale Mixer Mill MM301 (Retsch, Germany) for 5 min. The extracted wood powder was finely ball-milled in a Mono Mill P-6 (Fritsch, Japan) centrifugal ball at 550 rpm for 3 h. Approximately 0.4 g of finely ball-milled wood powder was suspended in 2.8 ml DMSO-d6 in a plastic tube and sonicated in an ultrasonic cleaning bath for 30 min. In the next step, the diluted solution was moved to an NMR tube. The 2D NMR spectra were recorded using a Bruker AVANCE III 600 MHz UltraShield instrument (Bruker, Germany) operated at 600 MHz. Bruker pulse program hsqcedetgpsp.3 was used for the HSQC experiments. The spectral widths were 16 ppm (9615 Hz) and 100 ppm (15,091 Hz) for the 1H(δH)- and 13C(δC)-dimensions, respectively. The number of points (TD) was 2048 for the 1H-dimension with a recycle delay of 1.2 s. The number of transients was 12, and 256-time increments were recorded in the 13C-dimension. The optimum direct coupling (1JCH) used was 145 Hz.

A semi-quantitative analysis of the integrals of the HSQC cross signal was conducted using Bruker Topspin 3.5 NMR software, and calculated based on the number of 100 aromatic units:

\(\left( {{\text{S}}+{\text{S}}^\prime } \right)/{\text{2}}+{\text{G}}+{\text{G}^\prime}={\text{total integral of aromatics}}.\)

Relative integral value of the specific signal × per 100 aromatic units = 100 × (Integral-X)/(total integral of aromatics).

where S is syringyl lignins; G is guaiacyl lignins; S, S′ is the integration of S2,6, S′2,6, respectively. G, G′ is the integration of G2, G′2, respectively.

Results and discussion

Mass losses

All wood specimens were completely colonized by external mycelia after 4 weeks of incubation. The mean percentage mass losses of two wood species caused by different white-rot fungi are shown in Fig. 1. The mean mass loss of E. fordii wood caused by P. chrysosporium and P. sordida was only 2%. However, these values for beech wood were 12 and 14%, respectively. After exposure to these fungi, the tested samples were subjected to further microscopic observations and chemical analyses.

Fig. 1
figure 1

Mean percentage mass loss in Erythrophleum fordii Oliver wood and beech wood after 4 weeks of exposure to two white-rot fungi. Error bars represent the standard error

Light and SEM microscopic observations

Microscopic examinations of the woody biomass revealed different patterns of degradation between E. fordii wood and beech wood (Figs. 2, 3). A little degradation was observed in the vessel and parenchymal cells of E. fordii wood (Fig. 2a1, a2), while vessels and fiber cells of beech wood were deeply eroded by P. chrysosporium (Fig. 2c1, c2). P. sordida caused defibrillation through dissolution of the middle lamella in some parenchyma of E. fordii wood, while fiber areas were still intact (Fig. 2b1, b2). Besides, the defibrillation process occurred in several beech wood tissues (Fig. 2d2). The E. fordii wood had very thick-walled fibers with the fiber lumina almost completely closed (Fig. 2a1). Because of the uniqueness of fiber structure, there was a little space for the development of hypha and hence limited the fungal degradation process.

Fig. 2
figure 2

Cross sections of E. fordii wood and beech wood after 4-week exposure to white-rot fungi: a1a2 E. fordii wood exposed to P. chrysosporium; b1b2 E. fordii wood exposed to P. sordida; c1c2 beech wood exposed to P. chrysosporium; d1d2 beech wood exposed to P. sordida. Hyphal colonization in vessels lumina (v), axial parenchymas (p), and fibers (f) (arrows), especially vessels (arrow). Erosion and rupture in cell walls and defibrillation (arrowheads). a1, c1, and d1: bar 100 µm; b1: bar 50 µm; a2, b2, c2, d2: bar 20 µm

Fig. 3
figure 3

Scanning electron micrographs of E. fordii and beech wood samples: (a1a3) E. fordii wood exposed to P. chrysosporium; b1b3 E. fordii wood exposed to P. sordida; c1c3 beech wood exposed to P. chrysosporium; d1d3 beech wood exposed to P. sordida; a1a2, b1b2, c1c2 and d1d2 cross sections: Colonization of hyphae in the lumen of vessels and fibers (arrows), deterioration of parenchyma (a2 arrowhead), and defibrillation of wood tissue (b2, d2 arrowheads). a3, b3, c2c3, and d3 Radial sections: the presence of hyphae in the lumen of vessels and parenchymal cells (a3) or vessels and fibers (c2); b3, c3 hyphae penetration in vessel pits and bore holes in vessel walls (arrowheads). a1, b1 Bar 100 µm; a2a3, b2b3, c1c3, and d1d3 bar 10 µm

During the decay process, changes in the structure of wood were hard to observe using light microscopy, while SEM clearly showed how the cell lumina were occupied by fungal hyphae. Hyphae were only observed in the lumen of vessels and parenchymal cells of E. fordii wood, while the fibers remained undamaged (Fig. 3a1, b1). Conversely, the hyphae were extended over whole tissues in beech wood (Fig. 3c1, d1). The difference in decay mechanism of P. chrysosporium and P. sordida was obviously witnessed. The P. chrysosporium showed no selectivity to lignocellulose. The wood cell walls were eroded and the middle lamellae were degraded by activity of P. chrysosporium (Fig. 3a2, c1). This is different from P. sordida white-rot fungi which preferentially degraded lignin instead of polysaccharides, causing defibrillation of wood (Fig. 3d1–d3). These microscopic observations are consistent with previously reported [20, 38,39,40]. SEM observations revealed that fungi colonized its hyphae in vessel members and then penetrated the neighboring parenchyma cells of E. fordii (Fig. 3a3) or fiber cells of beech wood (Fig. 3c2) to promote degradation. Furthermore, observations in the radial direction showed the penetration of hyphae from vessel lumen into adjacent cells via vessel pits (Fig. 3b3), and vessel-ray pits were destroyed by fungal activity (Fig. 3c3). This observation was supported by findings from the previous studies, which reported that hyphae tended to colonize the vessel lumen of infected hardwoods [41], then branch through simple or bordered pits to open pits, and resolve the hyphal penetration [16, 42].

Chemical characterization

Natural resistance to decay is one of the most important properties of wood, and is affected by the combination of wood density and the content and composition of lignin and extractives [43,44,45]. The antifungal test of E. fordii wood extractives was also performed. The results showed that E. fordii extractives were unable to inhibit the growth of fungi (see supplemental data). Therefore, the lignin structure is thought to be critical for resistance to degradation.

Lignin monomer composition and distribution among cell types and within different cell layers were the chemical parameters determining wood durability [46]. Based on TAPPI T222 om-98 examination, E. fordii wood had a higher Klason lignin content than beech wood. These values are 33.4% (0.14) and 20.6% (0.15) for E. fordii and beech wood, respectively. The numbers given in the brackets are standard deviation. Figure 4a–f shows the 2D HSQC NMR spectra of the whole nondegraded and degraded woods obtained at the solution stage in DMSO-d6. The main lignin substructures identified are also shown. The different lignin and polysaccharide cross signals assigned on the spectra are listed in Table 1, as previously described [23, 47, 48]. The results of a semi-quantitative analysis of the integrals of the HSQC cross signal are listed in Table 2. The differences between the spectra of fungal-degraded and nondegraded woods were observed. Cross peaks were observed at 106.2/6.47 (Gc) and 107.4/6.26 (G′c) ppm, which can be assigned to guaiacyl-condensed units. This is due to that correlation around 6.5/110 ppm can be assigned to C6–H6 correlation of 4-O-5 structures based on the NMR data of lignin models, as previously reported [47]. However, the presence of the equivalent G units could not be unequivocally established. The most significant difference between lignin structures of E. fordii wood and beech wood was that the former had a new signal determined as G′′c (Table 2). Although this signal was slightly shifted to 108.5/6.90 ppm, it can be assigned to C2–H2 correlations on the aromatic rings of 4-O-5-linked unit [47, 48]. It is also indicated that the structure of condensed lignin contents a substituent in the C-5 position, e.g., 5–5, β-5, and 4-O-5 structures [49]. The G/S-ratios of E. fordii were also higher than those of beech wood. Therefore, the main difference in the molecular structures of E. fordii and beech wood is the more condensed nature of E. fordii lignin. The localization and structure of lignin are important wood properties, because G are more strongly cross-linked, and therefore, more resistant to chemical degradation than lignin with a high S [43].

Fig. 4
figure 4

2D HSQC NMR experiments in the solution state of: a nondegraded E. fordii wood; b E. fordii wood degraded by P. chrysosporium; and c E. fordii wood degraded by P. sordida; d nondegraded beech wood; e beech wood degraded by P. chrysosporium; and f beech wood degraded by P. sordida. The main lignin structures identified are also shown: A β-O-4′ substructure; B resinol substructure; C phenylcoumaran substructure; D spirodienone substructure; G guaiacyl unit; G’ Cα-oxidized G unit; S syringyl unit; S′ Cα-oxidized S unit (R, lignin or OH; R′, H or lignin). See Table 1 for signal assignment

Table 1 Assignment of lignin and polysaccharide 1H–13C correlation signals in the HSQC spectra shown in Fig. 4
Table 2 Semi-quantitative analysis of the integrals of the HSQC cross signal of lignin and carbohydrates

Analysis of the 2D HSQC NMR spectra of E. fordii wood (Fig. 4a–c) before and after fungal exposure revealed similar signals for lignin and polysaccharide moieties, including cellulose and hemicelluloses. The lignin intensities of degraded woods with their characteristic one-bond δC/δH correlation at 108.5/6.90 (G′′c) were lower than those of nondegraded samples. On the other hand, the polysaccharide signals at 100.5/4.56 (M1), 73.1/4.46 (X′2), and 74.8/4.80 (X′3) ppm of degraded and nondegraded samples were substantially less significant. Besides, these lignin and polysaccharide intensities of E. fordii wood exposed to P. chrysosporium were slightly lower than those of P. sordida.

In contrast to E. fordii wood, analysis of the 2D HSQC NMR spectrum of beech wood revealed a significant decrease in both lignin and polysaccharide signals (Fig. 4d–f). The C2–H2 and C6–H6 in syringyl units were lower in treated woods because of fungal activity. The Cβ–Hβ correlation in β-O-4′ substructures (Aβ) was observed at δC/δH 83.5/4.30 for β-O-4′ substructures linked to S units, at δC/δH 86.3/4.04 for β-O-4′ substructures linked to G units, which decreased after the fungal test. Decreasing signals of other lignin substructures were also identified in the HSQC spectra. A strong signal for resinols (Bα), phenylcoumaran (β-5′) substructures (Cα), and spirodienone (Dα) decreased in the wood treated with P. chrysosporium, while those values were relatively unchanged in the wood exposured to P. sordida. In addition to lignin removal, the NMR spectrum of the white-rotted wood also revealed a simultaneous increase in polysaccharide signal, especially for wood treated with P. chrysosporium. This may be because P. chrysosporium produced more extracellular slime called sheath, composed of β-glucan, in the sample analyzed and that the signal was observed at δC/δH 103.1/4.30 for β-d-glucopyranoside. While variations do exist concerning wood species, P. chrysosporium is well known to be a simultaneous white-rot fungus causing decay of lignin, cellulose, and hemicelluloses at the same rates. The P. sordida exhibited preferential delignification, as reported previously [20, 46,47,48]. These findings are consistent with microscopic observation.

Wooden cultural properties are degraded by fungi (brown-rot, white-rot, and soft-rot fungi) and bacteria (erosion, tunneling, cavitation, etc.). These processes, at the same time, are driven by different environmental factors which have not been explicitly explored within this study. Although further examinations are needed, the characteristic of a fungi-resistance E. fordii wood presented in this study is of particular relevance to the highly condensed lignin content as well as the compactness of wood fibers. This explains why E. fordii wood can survive underground for centuries, even for a millennium. The internal part of excavated E. fordii wood is sometimes intact and does not need to be preserved. In such cases, conservation of the degraded outer layer with an appropriate consolidation agent may be sufficient for this particular wood species.

References

  1. Du D, Qu J, Wang JM, Yu SS, Chen XG, Xu S, Ma SG, Li Y, Ding GZ, Fang L (2010) Cytotoxic cassaine diterpenoid-diterpenoid amide dimers and diterpenoid amides from the leaves of Erythrophleum fordii. Phytochemistry 71:1749–1755

    Article  CAS  Google Scholar 

  2. Nan LI, Fang YU, Shi-shan YU (2004) Triterpenoids from Erythrophleum fordii. Acta Bot Sin 46:371–374

    Google Scholar 

  3. Cheng JS, Zhen S (1987) Chinese virose plant. Science Press, Beijing

    Google Scholar 

  4. Ha MT, Tran MH, Phuong TT, Kim JA, Woo MH, Choi JS, Lee S, Lee JH, Lee HK, Min BS (2017) Cytotoxic and apoptosis-inducing activities against human lung cancer cell lines of cassaine diterpenoids from the bark of Erythrophleum fordii. Bioorganic Med Chem Lett 27:2946–2952

    Article  CAS  Google Scholar 

  5. Hung TM, Cuong TD, Kim JA, Tae N, Lee JH, Min BS (2014) Cassaine diterpene alkaloids from Erythrophleum fordii and their anti-angiogenic effect. Bioorganic Med Chem Lett 24:168–172

    Article  CAS  Google Scholar 

  6. Qu J, Hu YC, Yu SS, Chen XG, Li Y (2006) New cassaine diterpenoid amides with cytotoxic activities from the bark of Erythrophleum fordii. Planta Med 72:442–449

    Article  CAS  Google Scholar 

  7. Tsao CC, Shen YC, Su CR, Li CY, Liou MJ, Dung NX, Wu TS (2008) New diterpenoids and the bioactivity of Erythrophleum fordii. Bioorganic Med Chem Lett 16:9867–9870

    Article  CAS  Google Scholar 

  8. Zhigang Z, Junjie G, Er S, Jie Z, Jianmin X (2012) Natural distribution, endangered mechanism and conservation strategy of an endangered tree species, Erythrophleum fordii Oliv. In: Asia and the Pacific workshop—multinational and transboundary conservation of valuable and endangered forest tree species. IUFRO Headquarters, Vienna, pp 113–116

    Google Scholar 

  9. Chen T (1988) Flora of China. Science Press, Beijing

    Google Scholar 

  10. Fang XF, Fang BZ (2007) Wood physical and mechanical properties of Erythrophleum fordii in southern Fujian (In Chinese). J Fujian Forest Sci Technol 34:146–147

    Google Scholar 

  11. QD 2198-CN (1997) Classification of Vietnamese timber (in Vietnamese). Ministry of Agriculture and Rural Development, Vietnam

    Google Scholar 

  12. Bich DTN, Phuong LX, Nguyet NTM, Thu PTT, Nam NQ, Chuong PV (2011) Fundamental properties of some wooden objetcs excavated in Thang Long Imperial Citadel (in Vietnamese). Vietnam National University of Forestry, Vietnam

    Google Scholar 

  13. Mtui W, Nokes SE (2014) Lignocellulolytic enzymes from tropical fungi: Types, substrates and applications. Sci Res Essays 7(15):1544–1555

    Google Scholar 

  14. Mahajan S (2011) Characterization of the white-rot fungus Phanerochaete carnosa through proteomic methods and compositional analysis of decayed wood fibre. PhD thesis, University of Toronto, Canada

  15. Jones D, Brischke C (2017) Performance of bio-based building materials. Woodhead Publishing Series in Civil and Structural Engineering. UK

    Book  Google Scholar 

  16. Bari E, Nazarnezhad N, Kazemi SM, Ghanbary MAT, Mohebby B, Schmidt O, Clausen CA (2015) Comparison between degradation capabilities of the white rot fungi Pleurotus ostreatus and Trametes versicolor in beech wood. Int Biodeterior Biodegrad 104:231–237

    Article  CAS  Google Scholar 

  17. Blanchette RA (1984) Screening wood decayed by white rot fungi for preferential lignin degradation. Appl Environ Microbiol 48:647–653

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Blanchette RA (1984) Selective delignification of eastern hemlock by Ganoderma tsugae. Phytopathology 74:153–160

    Article  CAS  Google Scholar 

  19. Cowling EB (1961) Comparative biochemistry of the decay of sweetgum sapwood by white-rot and brown-rot fungi. USDA Forest Service, Washington, DC

    Google Scholar 

  20. Schmidt O (2006) Wood and tree fungi: Biology, damage, protection, and use. Springer, Germany

    Google Scholar 

  21. Eriksson KEL, Blanchette RA, Ander P (1990) Microbial and enzymatic degradation of wood and wood components. Springer, Germany

    Book  Google Scholar 

  22. Blanchette RA, Nilsson T, Daniel G, Abad A (1990) Biological degradation of wood. In: Rowell RM, Barbour RJ (eds) Archaeological Wood. Advances in Chemistry Series, No. 225. American Chemical Society, Washington, DC, pp 141–192

    Chapter  Google Scholar 

  23. Martínez AT, Rencoret J, Nieto L, Jiménez-Barbero J, Gutiérrez A, Del Río JC (2011) Selective lignin and polysaccharide removal in natural fungal decay of wood as evidenced by in situ structural analyses. Environ Microbiol 13:96–107

    Article  Google Scholar 

  24. Anagnost SE (1998) Light microscopic diagnosis of wood decay. IAWA J 19:141–167

    Article  Google Scholar 

  25. Takano M, Hayashi N, Nakamura M, Yamaguchi M (2009) Extracellular peroxidase reaction at hyphal tips of white-rot fungus Phanerochaete crassa WD1694 and in fungal slime. J Wood Sci 55:302–307

    Article  CAS  Google Scholar 

  26. Pelit H, Yalçın M (2017) Resistance of mechanically densified and thermally post-treated pine sapwood to wood decay fungi. J Wood Sci 63:514–522

    Article  CAS  Google Scholar 

  27. Tsujiyama S (2001) Differential scanning calorimetric analysis of the lignin-carbohydrate complex degraded by wood-rotting fungi. J Wood Sci 47:497–501

    Article  CAS  Google Scholar 

  28. Kim NH (2005) An investigation of mercerization in decayed oak wood by a white rot fungus (Lentinula edodes). J Wood Sci 51:290–294

    Article  CAS  Google Scholar 

  29. Nishimura H, Sasaki M, Seike H, Nakamura M, Watanabe T (2012) Alkadienyl and alkenyl itaconic acids (ceriporic acids G and H) from the selective white fungus Ceriporiopsis subvermispora: A new class of metabolites initiating ligninolytic lipid peroxidation. Org Biomol Chem 10:6432–6442

    Article  CAS  Google Scholar 

  30. Zabell RA, Morrell JJ (1992) Wood microbiology, decay and its prevention. Academic Press, New York

    Google Scholar 

  31. Nishimura H, Yamaguchi D, Watanabe T (2017) Cerebrosides, extracellular glycolipids secreted by the selective lignin-degrading fungus Ceriporiopsis subvermispora. Chem Phys Lipids 203:1–11

    Article  CAS  Google Scholar 

  32. Pandey KK, Pitman AJ (2003) FTIR studies of the changes in wood following decay by brown-rot and white-rot fungi. Int Biodeterior Biodegradation 52:151–160

    Article  CAS  Google Scholar 

  33. Kim H, Ralph J, Akiyama T (2008) Solution-state 2D NMR of ball-milled plant cell wall gels in DMSO-d 6. Org Biomol Chem 1:56–66

    Google Scholar 

  34. Ralph J, Marita JM, Ralph SA, Hatfield RD, Lu F, Ede RM, Peng J, Quideau S, Helm RF, Grabber J, Kim H, Jimenez-Monteon G, Zhang Y, Jung HJG, Landucci L, MacKay J, Sederoff R, Chapple C, Boudet A (1999) Solution-state NMR of lignins. In: Argyropoulos DS (ed) Advances in lignocellulosics characterization. TAPPI Press, Atlanta, pp 55–108

    Google Scholar 

  35. Rencoret J, Marques G, Gutiérrez A, Nieto L, Santos JI, Jiménez-Barbero J, Martínez AT, Río JC (2009) HSQC-NMR analysis of lignin in woody (Eucalyptus globulus and Picea abies) and non-woody (Agave sisalana) ball-milled plant materials at the gel state. Holzforschung 63:691–698

    Article  CAS  Google Scholar 

  36. Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43

    Article  CAS  Google Scholar 

  37. TAPPI T222 om-98 (1998) Standard methods for Acid-insoluble lignin in wood and pulp. TAPPI Press, Atlanta

    Google Scholar 

  38. Burdsall HH (1985) A contribution to the taxonomy of the genus Phanerochaete (Corticiaceae, Aphyllophorales). J. Cramer Publisher, Braunschweig

    Google Scholar 

  39. Koyani RD, Rajput KS (2014) Light microscopic analysis of Tectona grandis L.f. wood inoculated with Irpex lacteus and Phanerochaete chrysosporium. Eur J Wood Prod 72:157–164

    Article  CAS  Google Scholar 

  40. Schwanninger M, Rodrigues JC, Pereira H, Hinterstoisser B (2004) Effects of short-time vibratory ball milling on the shape of FT-IR spectra of wood and cellulose. Vib Spectrosc 36:23–40

    Article  CAS  Google Scholar 

  41. Wilcox WW (1970) Anatomical changes in wood cell walls attacked by fungi and bacteria. Bot Rev 36:1–28

    Article  Google Scholar 

  42. Schwarze FWMR (2007) Wood decay under the microscope. Fungal Biol Rev 21:133–170

    Article  Google Scholar 

  43. Nuopponen MH, Wikberg HI, Birch GM, Jääskeläinen AS, Maunu SL, Vuorinen T, Stewart D (2006) Characterization of 25 tropical hardwoods with Fourier transform infrared, ultraviolet resonance Raman, and 13C-NMR cross-polarization/magic-angle spinning spectroscopy. J App Pol Sci 102:810–819

    Article  CAS  Google Scholar 

  44. Oliveira LS, Santana ALBD, Maranhão CA, Miranda RDCM, Lima VLAG, Silva SI, Nascimento MS, Bieber L (2010) Natural resistance of five woods to Phanerochaete chrysosporium degradation. Int Biodeterior Biodegrad 64:711–715

    Article  Google Scholar 

  45. Onuorach EO (2000) The wood preservative potentials of heartwood extracts of Milicia excelsa and Erythrophleum suaveolens. Bioresour Technol 75:171–173

    Article  Google Scholar 

  46. Skyba O, Douglas CJ, Mansfield SD (2013) Syringyl-rich lignin renders poplars more resistant to degradation by wood decay fungi. Appl Environ Microbiol 79(8):2560–2571

    Article  CAS  Google Scholar 

  47. Li Y, Akiyama T, Yokoyama T, Matsumoto Y (2016) NMR assignment for diaryl ether structures (4 – O–5 structures) in pine wood lignin. Biomacromol 17:1921–1929

    Article  CAS  Google Scholar 

  48. Yue F, Lu F, Ralph S, Ralp J (2016) Identification of 4-O-5-Units in softwood lignins via definitive lignin models and NMR. Biomacromol 17:1909–1920

    Article  CAS  Google Scholar 

  49. Heitner C, Dimmel D, Schmidt JA (2010) Lignin and lignans: advances in chemistry. CRC Press, London

    Book  Google Scholar 

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Acknowledgements

This study was partly supported by the Grants-in-Aid for Scientific Research (A) No. 25252033, Japan Society for the Promotion of Science, RISH Cooperative Research (database) and RISH Mission Research V, Kyoto University. The authors acknowledge the Xylarium, RISH, Kyoto University, Japan, and Vietnamese Academy of Forest Sciences, Hanoi, Vietnam for providing the wood materials.

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Correspondence to Junji Sugiyama.

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Nguyen, T.D., Nishimura, H., Imai, T. et al. Natural durability of the culturally and historically important timber: Erythrophleum fordii wood against white-rot fungi. J Wood Sci 64, 301–310 (2018). https://doi.org/10.1007/s10086-018-1704-1

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  • DOI: https://doi.org/10.1007/s10086-018-1704-1

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