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Official Journal of the Japan Wood Research Society

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Table 2 Elemental composition and higher heating values (HHVs) of transgenic rice cell walls and synthetic lignins

From: Comparative evaluations of lignocellulose reactivity and usability in transgenic rice plants with altered lignin composition

  Elemental composition (%) HHVb (J/g)
C H O N By elemental composition By bomb calorimetry
Cell wall residue (CWR)
 WT 44.8 ± 0.1 5.9 ± 0.0 48.9 ± 0.1 0.4 ± 0.1 18,100 ± 30B 18,760 ± 400A
 OsCAld5H1-KD 44.2 ± 0.1 6.0 ± 0.0 49.4 ± 0.2 0.4 ± 0.1 17,970 ± 40A,B 18,820 ± 790A
 OsCAld5H1-OX 44.8 ± 0.1 6.0 ± 0.0 48.2 ± 0.2 0.9 ± 0.0 18,190 ± 40B 19,560 ± 910A,B
 OsC3´H1-KD 43.8 ± 0.1 5.9 ± 0.0 49.4 ± 0.1 0.9 ± 0.0 17,820 ± 80A 18,330 ± 370A
Lignin-enriched CWRa
 WT 52.9 ± 0.1 5.8 ± 0.1 40.3 ± 0.1 1.0 ± 0.0 20,360 ± 70C 22,080 ± 1430B,C
 OsCAld5H1-KD 54.1 ± 0.0 5.8 ± 0.0 39.0 ± 0.1 1.1 ± 0.0 20,710 ± 50D 22,650 ± 340C
 OsCAld5H1-OX 52.3 ± 0.1 5.8 ± 0.1 40.2 ± 0.2 1.7 ± 0.0 20,220 ± 90C 21,640 ± 1410B,C
 OsC3´H1-KD 53.7 ± 0.4 5.9 ± 0.2 38.1 ± 0.6 2.4 ± 0.2 20,710 ± 190D 20,920 ± 1950A,C
Synthetic lignin
 G-DHP 59.1 ± 0.2 5.4 ± 0.1 35.3 ± 0.2 0.2 ± 0.0 21,880 ± 70F ND
 S-DHP 56.0 ± 0.1 5.8 ± 0.0 36.5 ± 0.0 1.7 ± 0.1 21,280 ± 40E ND
 H-DHP 65.3 ± 0.1 5.4 ± 0.1 28.7 ± 0.2 0.6 ± 0.0 23,720 ± 110G ND
  1. ND, not determined; WT, wild-type; OsCAld5H1-KD, OsCAld5H1-knockdown; OsCAld5H1-OX, OsCAld5H1-overexpression; OsC3´H1-KD, OsC3´H1-knockdown
  2. aCell wall residues after enzymatic hydrolysis of the polysaccharide fraction with crude cellulases. Isolation yields and cell wall sugar composition are shown in Additional file 1: Table S2
  3. bDifferent letters indicate a significant difference between datasets (ANOVA with post hoc Tukey–Kramer method, p < 0.05). Elemental composition and HHV results are reported on an ash-free basis. Values are mean ± SD (n = 3)