Journal of Wood Science

Official Journal of the Japan Wood Research Society

Table 2 Elemental composition and higher heating values (HHVs) of transgenic rice cell walls and synthetic lignins

From: Comparative evaluations of lignocellulose reactivity and usability in transgenic rice plants with altered lignin composition

	Elemental composition (%)				HHV^b (J/g)
	C	H	O	N	By elemental composition	By bomb calorimetry
Cell wall residue (CWR)
WT	44.8 ± 0.1	5.9 ± 0.0	48.9 ± 0.1	0.4 ± 0.1	18,100 ± 30^B	18,760 ± 400^A
OsCAld5H1-KD	44.2 ± 0.1	6.0 ± 0.0	49.4 ± 0.2	0.4 ± 0.1	17,970 ± 40^A,B	18,820 ± 790^A
OsCAld5H1-OX	44.8 ± 0.1	6.0 ± 0.0	48.2 ± 0.2	0.9 ± 0.0	18,190 ± 40^B	19,560 ± 910^A,B
OsC3´H1-KD	43.8 ± 0.1	5.9 ± 0.0	49.4 ± 0.1	0.9 ± 0.0	17,820 ± 80^A	18,330 ± 370^A
Lignin-enriched CWR^a
WT	52.9 ± 0.1	5.8 ± 0.1	40.3 ± 0.1	1.0 ± 0.0	20,360 ± 70^C	22,080 ± 1430^B,C
OsCAld5H1-KD	54.1 ± 0.0	5.8 ± 0.0	39.0 ± 0.1	1.1 ± 0.0	20,710 ± 50^D	22,650 ± 340^C
OsCAld5H1-OX	52.3 ± 0.1	5.8 ± 0.1	40.2 ± 0.2	1.7 ± 0.0	20,220 ± 90^C	21,640 ± 1410^B,C
OsC3´H1-KD	53.7 ± 0.4	5.9 ± 0.2	38.1 ± 0.6	2.4 ± 0.2	20,710 ± 190^D	20,920 ± 1950^A,C
Synthetic lignin
G-DHP	59.1 ± 0.2	5.4 ± 0.1	35.3 ± 0.2	0.2 ± 0.0	21,880 ± 70^F	ND
S-DHP	56.0 ± 0.1	5.8 ± 0.0	36.5 ± 0.0	1.7 ± 0.1	21,280 ± 40^E	ND
H-DHP	65.3 ± 0.1	5.4 ± 0.1	28.7 ± 0.2	0.6 ± 0.0	23,720 ± 110^G	ND

ND, not determined; WT, wild-type; OsCAld5H1-KD, OsCAld5H1-knockdown; OsCAld5H1-OX, OsCAld5H1-overexpression; OsC3´H1-KD, OsC3´H1-knockdown
^aCell wall residues after enzymatic hydrolysis of the polysaccharide fraction with crude cellulases. Isolation yields and cell wall sugar composition are shown in Additional file 1: Table S2
^bDifferent letters indicate a significant difference between datasets (ANOVA with post hoc Tukey–Kramer method, p < 0.05). Elemental composition and HHV results are reported on an ash-free basis. Values are mean ± SD (n = 3)

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