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Official Journal of the Japan Wood Research Society

Structural basis of lignocellulose deconstruction by the wood-feeding anobiid beetle Nicobium hirtum

Abstract

The details of the lignocellulose deconstruction processes in the digestive systems of wood-feeding insects remain elusive. This study aimed to examine the biochemical conversion of lignocellulose in the digestive system of a wood-feeding anobiid beetle, Nicobium hirtum, one of the most important pests of wooden products in Japan. To this end, N. hirtum larvae were fed with Japanese red pine (softwood) and Japanese beech (hardwood) sapwood diets, as well as an artificial diet containing Shorea wood (hardwood) sapwood sawdust. The structural differences between the original and digested (feces) lignocellulose samples were examined using wet-chemical and two-dimensional (2D) nuclear magnetic resonance (NMR) methods. Cellulose and hemicelluloses, especially mannan in the softwood diet, were preferentially degraded over lignin in the larval digestive system. As a result, lignin was enriched in the digested lignocellulose residues. Lignin compositional analyses based on thioacidolysis and 2D NMR determined that the proportions of oxidized lignin aromatic units were notably increased after digestion. Further, the 2D NMR analyses revealed the accumulation of aldehyde and hydroxypropiovanillone/syringone end-unit structures in lignin, indicating that oxidative and/or reductive modifications of lignin polymers occur in the larval digestive system. Such structural alterations of lignin may facilitate the dissociation of the lignin barrier, thereby liberating polysaccharides for subsequent enzymatic conversion for assimilation and energy.

Introduction

Wood-feeding or xylophagous insects are mainly distributed in the three major orders of Insecta, i.e., Coleoptera (beetles), Blattodea (cockroaches and termites) and Hymenoptera (ants, bees, and wasps) [1]. They comprise important wood consumers (e.g., termites) that have a significant impact on wood decay and carbon recycling in terrestrial ecosystems [2]. Many are also recognized as wood pests because they can inflict severe damage on economically important forest trees and dry wood materials [1]. These wood-feeding insects have developed unique and diverse digestive systems, often associated with intestinal microbial symbionts, which can produce the enzymes necessary to decompose lignocellulose, the recalcitrant biocomposite of cellulose, hemicelluloses, and lignin that constitutes woody plant cell walls [2,3,4,5,6]. There is much to learn about the mechanisms underlying lignocellulose digestion by wood-feeding insects, i.e., how they achieve efficient hydrolysis and subsequent conversion of polysaccharides (cellulose and hemicelluloses) for assimilation and energy, and also how they circumvent recalcitrant lignin barriers to gain access to the embedded polysaccharides. These topics are receiving considerable research attention, not only to better understand the physiology and evolution of herbivorous insects [7], but also to develop biotechnological strategies for wood pest management [6] and methods to produce biochemicals from lignocellulosic biomass [8].

In recent years, lignocellulose deconstruction processes in insect gut digestive systems have been revisited through structural analyses of digested lignocellulose residues in feces using advanced analytical techniques. In particular, recent studies employing pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) [9,10,11,12,13] and/or two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy [13,14,15] have detected differential degradation patterns of cell wall polysaccharides, i.e., cellulose and hemicelluloses, as well as evidence of previously overlooked chemical alterations of lignin polymers during their passage through the insect digestive systems. Nevertheless, these structural studies are still limited to a small number of insect species, mostly termites (Blattodea). With regard to beetles (Coleoptera), although significant advances have been made in understanding the genetic and biochemical aspects of their lignocellulose degradation enzymes and gut microbial consortia [2, 12, 16], structural basis of lignocellulose deconstruction in their digestive systems is yet to be defined. In this study, we close this gap by analyzing the digestive processes of the anobiid beetle, Nicobium hirtum (Coleoptera: Ptinidae).

N. hirtum is a representative species of “death-watch beetles” or “shiban-mushi” (in Japanese) found in Japan. It is one of the most important wood pests, especially for cultural wooden structures and artifacts, in the country [17,18,19]. It prefers seasoned wood and subsists on both softwood and hardwood, apparently indiscriminately [17, 18]. The attacked wood can be recognized by 2–3 mm round holes on the surface (Fig. 1a) and, more indicatively, by the cylindrical fecal pellets (frass) produced by the larvae (Fig. 1b). The adult beetles are 3 to 7 mm long and reddish-to-blackish brown with slender antennae that consist of 11 flattened segments (Fig. 1c). The larvae are typically up to 7 mm long, with a creamy-white scarabaeiform (c-shaped) appearance (Fig. 1d). Although both the adult beetles and larvae have powerful toothed mandibles that enable them to bore wood (Fig. 1c, d), most damage is inflicted by larvae nesting inside woody tissues and using lignocellulose as the major carbon source. Although many Anobiidae members have been observed to utilize plant cell walls, little is yet known about the effects of different diets on the physiology of N. hirtum, and details of their lignocellulose degradation ability are largely unknown, despite their importance as notorious wood pests. In this study, we used a recently developed laboratory culture of N. hirtum [19] to conduct basic physiological characterizations of N. hirtum larvae fed on different lignocellulose diets. Further, we conducted in-depth structural characterizations of lignocellulose in their feces using a series of wet chemistry and 2D NMR methods.

Fig. 1
figure 1

Damage caused by Nicobium hirtum and appearance of adults and larvae. Appearance of wooden furniture attacked by N. hirtum (a), cylindrical larval fecal pellets (b), and morphology of N. hirtum adult beetle (c) and larva (d). Inserts are magnified facial views showing toothed mandibles of N. hirtum adult beetle (c) and larva (d)

Materials and methods

Larvae feeding and feces collection

The N. hirtum larvae were obtained from a laboratory colony recently developed at the Deterioration Organisms Laboratory (DOL), Research Institute for Sustainable Humanosphere, Kyoto University, Japan [19]. Sixty middle-stage instars of N. hirtum larvae were collected from the colony and placed on sapwood blocks [2 (L) × 2 (W) × 2 (H) cm] of Japanese red pine (Pinus densiflora) or Japanese beech (Fagus crenata), or artificial diet blocks [2 (L) × 2 (W) × 2 (H) cm]. The artificial diet consisted of 50% (w/w) starch, 24% (w/w) yeast extract, and 26% (w/w) Shorea sp. sawdust, and was originally designed to feed mass cultures of Lyctus species [20,21,22]. Each larva was placed in a hole in the center of the diet block and allowed to grow in an incubator maintained at 25 °C and 65% relative humidity. The cylindrical fecal pellets (Fig. 1) were collected once a week for 5 months to obtain sufficient material for lignocellulose analysis. The collected feces were immediately frozen and kept at − 25 °C to prevent further degradation. After 5 months of feeding, the larvae survival rates were recorded, and the remaining diet blocks were oven-dried and weighed to determine the mass loss during the feeding period (Fig. 2). The obtained feces (digested) and original (undigested) diet samples were pulverized, washed successively with water and 80% ethanol, and then lyophilized to obtain cell wall residue (CWR) samples [15] for wet chemistry and 2D NMR analyses.

Fig. 2
figure 2

Survival, feeding, and growth of N. hirtum larvae fed with different diets. Larvae survival (a), lignocellulose diet weight loss (b), and larvae body mass (c) of N. hirtum larvae after 5 months of feeding on Chinese red pine (softwood), Japanese beech (hardwood), or a Shorea (hardwood) artificial diet. Values in b and c are means ± SD (n = 20). Different letters indicate significant differences (one-way ANOVA with Tukey HSD test, p < 0.05)

Chemical analyses

The starch content was determined by measuring the amount of glucose released by treatment with thermostable α-amylase and amyloglucosidase according to Hattori et al. [23]. The contents of crystalline cellulose and non-crystalline glycan were determined by the two-step acid hydrolysis method using trifluoroacetic acid and sulfuric acid as described by Lam et al. [24]. The thioglycolic acid lignin assay for determination of lignin content [25] and analytical thioacidolysis for determination of lignin composition [26] were performed as described previously.

2D NMR analyses

The CWR samples were finely ball-milled with a planetary micro mill (Pulverisette 7, Fritsch Industrialist, Idar-Oberstein, Germany) and swelled in 600 µL dimethyl sulfoxide (DMSO)-d6/pyridine-d5 (4:1, v/v) before whole-cell-wall NMR analysis [27,28,29]. Adiabatic 2D heteronuclear single quantum coherence (HSQC) NMR spectra were acquired using a Bruker Avance III 800US system (800 MHz, Bruker Biospin, Billerica, MA, USA) fitted with a cryogenically cooled 5 mm TCI gradient probe (Bruker Biospin) using the standard Bruker implementation method (‘hsqcetgpsp.3’) and acquisition parameters described previously [27,28,29]. Data processing and analysis were performed using Bruker TopSpin 4.1 (MacOS) software (Bruker Biospin) as described previously [13, 15, 30]. Peak assignments were conducted by comparison of chemical shift data with data reported in the literature [13, 27,28,29, 31]. For volume integration analysis of the lignin aromatic unit and polysaccharide anomeric signals (Fig. 3), C2–H2 correlations from guaiacyl units (G2 and G′2), C2–H2/C6–H6 correlations from syringyl units (S2/6 and S′2/6), and C1–H1 correlations from the major polysaccharide units (Gl1, M1, M′1, M″1, X1, X′1, X″1, X′′′1, A1, and U1) were manually integrated, and S2/6 and S′2/6 integrals were logically halved. For analysis of lignin inter-monomeric and end-unit linkage types (Fig. 4), well-resolved Cα–Hα contours from the lignin inter-monomeric linkages (Iα, IIα, and IIIα) and the benzaldehyde end-units (IV″α), Cγ–Hγ contours from the cinnamaldehyde end-units (IV′α), and Cβ–Hβ contours from the hydroxypropiovanilone (HPV)/hydroxypropiosyringone (HPS) end-units (IV′′′β) were also manually integrated, and IIIα and IV′′′β integrals were logically halved [13, 15, 30].

Fig. 3
figure 3

Lignin aromatic units and polysaccharides in original and digested diet samples of N. hirtum. Aromatic (a) and polysaccharide anomeric (b) sub-regions 2D HSQC NMR spectra of pine wood and Shorea artificial diet lignocellulose samples before (“original”) and after (“digested”) digestion by N. hirtum larvae. Figures show normalized signal intensity values of major lignin aromatic and polysaccharide anomeric signals expressed as percentages of the sum of the listed signals (see Materials and methods) (c) and ratios of non-oxidized and oxidized syringyl (½S2/6 and ½2/6) and guaiacyl (G2 and 2) lignin aromatic signals (d). Data labeled × 5 indicate that the reported values are multiplied by 5 for visualization purposes. Peak assignments are listed in Table 2

Fig. 4
figure 4

Lignin inter-monomeric and end-unit linkage types in undigested and digested diets of N. hirtum. Oxygenated aliphatic (a and c) and aldehyde (b) sub-regions of 2D HSQC NMR spectra of pine wood and Shorea artificial diet lignocellulose samples before (“original”) and after (“digested”) digestion by N. hirtum larvae. Boxes labeled × 2 represent regions with the scale vertically enlarged by twofold. In d, normalized signal intensity values of the major lignin inter-monomeric linkage types (Iɑ, IIɑ and ½IIIɑ) and aldehyde (IV′γ and IV′′ɑ) and HPV/HPS (½IV′′′β) end-unit signals expressed as percentages of Iɑ + IIɑ + ½IIIɑ are shown. Data labeled × 5 indicate that the reported values are multiplied by 5 for visualization purposes. Peak assignments are listed in Table 2

Statistical analysis

Student’s t-test (p < 0.05) and one-way analysis of variance (ANOVA) followed by Tukey’s honestly significant difference (HSD) test (p < 0.05) were performed using IBM SPSS statistics version 27 (IBM Corporation, Armonk, NY, USA).

Results

Physiological characteristics of N. hirtum larvae fed on lignocellulose diets

We recorded larvae survival, the decrease in the mass of the lignocellulose diet, and the growth (change in body mass) of the larvae fed on three different lignocellulose diets, i.e., plain sapwood blocks of Japanese red pine (softwood) and Japanese beech (hardwood), and an artificial diet block consisting of Shorea (hardwood) sapwood sawdust, yeast extract, and starch. Our preliminary experiments indicated that this Shorea wood-based artificial diet, which was originally developed for feeding powder-post beetles (Lyctus species) in the laboratory [20,21,22], is suitable for the maintenance of N. hirtum larvae [19]. The survival rates of the larvae fed with the pine wood and the Shorea artificial diet were similarly high (> 80%), whereas that of the larvae fed with the beech wood diet was considerably lower (ca. 40%) (Fig. 2a). Consistent with this result, the larvae consumed significantly less of the beech wood diet than the other two diets (Fig. 2b). The body mass of the larvae fed with the Shorea artificial diet was significantly higher than those of the larvae fed with the pine and beech plain wood diets (Fig. 2c). This result emphasizes the importance of starch and protein (yeast extract) as nutrients for the survival and growth of N. hirtum larvae, even though they subsist on, and utilize, lignocellulose as the major carbon source in nature.

Changes in lignocellulose composition after passage through the digestive system of N. hirtum larvae

To understand the lignocellulose deconstruction processes in the digestive system of N. hirtum larvae, we subjected the CWR samples prepared from the original and digested (feces) lignocellulose diets to a series of lignocellulose compositional analyses using wet-chemical methods. We could not collect sufficient feces for analyses from the larvae fed on the beech wood diet, mainly because of the low larval survival rate (Fig. 2a). Therefore, we report the results obtained for the digested pine wood and Shorea artificial diet samples. The cell wall structural data of the original beech wood diet sample used in this study were reported in our earlier study [15].

The sugar and lignin content analyses indicated that the digested pine wood and Shorea artificial diet lignocelluloses were both depleted in polysaccharides and proportionally augmented in lignin (Table 1). This result shows that, as reported for many other wood-feeding insects, N. hirtum larvae digest primarily polysaccharides, leaving a majority of lignin polymers in fecal digestive residues. For the pine wood diet that contained little starch (ca. 0.3% of CWR), the contents of crystalline glucan as well as mannan were notably reduced after digestion (Table 1), demonstrating that N. hirtum larvae can digest cellulose and hemicelluloses, especially mannan. For the Shorea artificial diet, which contained abundant starch, N. hirtum larvae preferentially digested starch over cellulose and hemicelluloses (Table 1). Nevertheless, we detected notable decreases in the contents of amorphous glucan and mannan in the digested Shorea artificial diet, indicative of the digestion of hemicellulosic glycans. Interestingly, xylan was significantly augmented in the digested samples of pine wood and Shorea artificial diets, compared with the original diets (Table 1). This result suggests that N. hirtum larvae are less able to digest xylan than other cell wall polysaccharide components, such as cellulosic glucan and hemicellulosic mannan, in lignocellulose.

Table 1 Composition of samples before (original) and after (digested) digestion by N. hirtum larvae

In-depth lignocellulose structural analyses by 2D NMR and thioacidolysis

To obtain further detailed chemical information on the digestion of lignocellulose diets by N. hirtum larvae, we performed in-depth cell wall structural analysis by collecting 2D HSQC NMR spectra of the whole CWR samples via the direct dissolution/swelling method with the DMSO-d6/pyridine-d5 solvent system [27, 28]. The obtained HSQC spectra of the pine wood and Shorea artificial diet lignocellulose samples displayed major lignin and polysaccharide signals typical of softwood and hardwood, respectively (Fig. 3). Peak assignments based on chemical shift data reported in the literature [13, 27,28,29, 31] are listed in Table 2.

Table 2 Assignment of polysaccharide and lignin signals in 1H–13C correlation (HSQC) spectra of pine and Shorea diet cell wall samples

The aromatic sub-regions (δC/δH, 140–100/8.0–6.0) of the pine wood HSQC spectra displayed aromatic signals typical of softwood lignin composed of only guaiacyl (G) units (G and ), whereas the spectra of the Shorea diets additionally displayed signals from syringyl (S) units (S and ) typical of hardwood lignin (Fig. 3a and Table 2). The polysaccharide anomeric sub-regions (δC/δH, 110–90/4.0–5.5) displayed anomeric signals associated with cellulose and hemicellulose: glucans (Gl); unacetylated (M) and acetylated ( and Mʹʹ) mannans (in the pine wood spectra); unacetylated (X) and acetylated (, Xʹʹ, and Xʹʹʹ) xylans (in the Shorea diet spectra); arabinan (A); glucuronan (U). In addition, the Shorea artificial diet spectra displayed intense signals from aromatic amino acids [31] and starch glucans, which could be attributed to the protein (yeast extract) and starch components of this diet (Fig. 3b and Table 2).

For comparison between the original and digested lignocellulose diet spectra, the major lignin aromatic and cell wall polysaccharide anomeric signals were integrated and normalized based on the sum of the integrated signals (Fig. 3c). Consistent with the chemical analysis data (Table 1), the lignin aromatic signals were all proportionally augmented, whereas polysaccharide signals, especially those from glucan and mannan, were depleted in the spectra of digested lignocellulose compared with the spectra of lignocellulose in the original diets (Fig. 3c). This result provided further evidence that N. hirtum larvae preferentially digested polysaccharides, especially cellulosic glucan and hemicellulosic mannan, over lignin in these lignocellulose diets. For the Shorea artificial diet spectra, signals from proteins (Fig. 3a) and starch glucan (Fig. 3b) were clearly depleted after digestion, although they are not included in the integration analysis data presented in Fig. 3c. This result confirmed the more efficient digestion of starch and protein than Shorea lignocellulose in this artificial diet.

The changes in lignin composition as a result of its passage through the N. hirtum larval digestive system were examined by comparing the HSQC signal intensities of the non-oxidized and oxidized G and S aromatic signals (G, , S and ) (Fig. 3d). In addition, we conducted a complementary lignin compositional analysis using thioacidolysis (Table 1), which quantifies lignin-derived monomeric compounds released from non-oxidized β–O–4 units in the lignin polymer [32]. The results of the 2D HSQC NMR (Fig. 3d) and thioacidolysis (Table 1) analyses consistently showed that the ratio of non-oxidized S and G lignin units (S/G ratio) was slightly reduced in the digested Shorea artificial diet compared with the original diet. It was noticeable that there were some deviations between the HSQC-derived S/G signal ratio and the thioacidolysis-derived S/G monomer ratio. This could be at least partly attributed to the fact that thioacidolysis measures only the S/G unit ratio in the monomers released by cleaving β–O–4 bonds, whereas HSQC NMR estimates the S/G unit distribution in the entire lignin polymers. Further, the HSQC NMR results also revealed that the oxidized G and S signals ( and ) were proportionally augmented over non-oxidized G and S signals (G and S) in the spectra of the digested pine wood and Shorea artificial diets (Fig. 3d). These results indicate that lignin can be structurally modified during its passage through the N. hirtum larvae digestive system, as further demonstrated below.

To further examine the lignin structures in the digested lignocellulose diets, we analyzed lignin inter-monomeric and end-unit linkage signals appearing in the oxygenated-aliphatic and aldehyde sub-regions of the HSQC NMR spectra (Fig. 4 and Table 2). Typical lignin linkage signals from β–O–4 (I), β–5 (II), and β–β (III) units were clearly visible in both the original and digested lignocellulose diet spectra (Fig. 4a). Volume integration analysis of the relatively well-resolved Cα–Hα signals revealed differences in the distributions of these major inter-monomeric linkage types between the original and digested lignin samples (Fig. 4d). For both the pine wood and Shorea artificial diets, the inter-monomeric linkage distribution patterns were similar between the original and digested lignocellulose, suggesting that the structural modifications occurring in the N. hirtum larvae digestive system were not drastic. However, for both of the lignocellulose diets, the β–O–4 unit signals (I) were proportionally decreased, whereas the β–5 (II) and β–β (III) signals were proportionally increased, after digestion (Fig. 4d). We also detected increases in the cinnamaldehyde (IVʹ) and benzaldehyde (IVʹʹ) end-units in the spectra of the digested lignocellulose samples (Fig. 4b and d). Moreover, a set of signals attributed to the HPV/HPS end-units (IVʹʹʹ), most diagnostically Cβ–Hβ (IVʹʹʹβ) at δC/δH = 41.2/3.11 and Cγ–Hγ (IVʹʹʹγ) at δC/δH = 57.2/3.81 [13, 33], was clearly detected in the digested pine wood spectrum, although the appearance of IVʹʹʹ in the Shorea artificial diet spectrum was unclear, probably because of the relatively low lignin content. In contrast, these HPV/HPS (IVʹʹʹ) signals were undetectable in the spectra of the original lignocellulose diets (Fig. 4a, c and d). The increments in these aldehydes and HPV/HPS lignin end-units were consistent with the increased signals of oxidized G and S lignin units in the digested lignocellulose (Fig. 3), providing further evidence that lignin polymers undergo partial structural modifications during their passage through the N. hirtum larvae digestive system.

Discussion

As reported for several other wood-feeding beetles [1, 8], the larvae of the anobiid beetle N. hirtum can subsist on plain softwood (pine) and hardwood (beech) lignocellulose diets and utilize them as a sole carbon source, although adding starch and protein into the hardwood (Shorea) lignocellulose diet can greatly promote their survival and growth (Fig. 2). The larvae physiology data suggested that N. hirtum larvae prefer softwood to hardwood as a lignocellulose-based carbon source. Although N. hirtum larvae attacks both softwood and hardwood, apparently indiscriminately [17, 18], some Anobiidae members reportedly attack softwoods preferentially over hardwoods [34,35,36]. Our chemical and 2D NMR analyses of the original and digested lignocellulose diets clearly demonstrated that N. hirtum larvae are able to decompose cell wall polysaccharides, i.e., cellulose and hemicelluloses. Upon feeding with the plain pine wood diet containing little residual starch, the larvae digested both cellulose and hemicelluloses along with residual starch. On the other hand, cellulose digestion was apparently limited when they were fed with the Shorea artificial diet containing additional starch and protein (Table 1 and Fig. 3), suggesting that, in the presence of rich starch and protein sources, the larvae preferentially digest them over cell wall polysaccharides. Intriguingly, among the hemicellulosic glycans present in the pine and Shorea diets, mannan and amorphous glucan were preferentially digested over other glycans by N. hirtum larvae (Table 1 and Fig. 3). The fact that N. hirtum larvae preferentially digest mannan and glucan, which represent the major hemicellulosic sugars in softwood (as glucomannan) [37], may be associated with the positive nutritional effects of the softwood diet over the hardwood diet on larval survival and growth (Fig. 2).

The ability of N. hirtum larvae to degrade cellulose and hemicellulose indicate potential roles of plant cell wall-degrading enzymes (PCWDEs) in the gut digestive system. Such enzymes may originate from either the beetle host or its gut-resident microbial symbionts, or both. Recent genomic studies have emphasized the importance of the endogenous PCWDEs in beetles [2, 3, 7, 16], many of which are thought to have originated from bacteria or fungi via horizontal gene transfer during the evolution and biodiversification of herbivorous beetles [16, 38]. Indeed, endogenous PCWDE genes, including those encoding glycoside hydrolase (GH) family proteins that typically show cellulolytic and hemicellulolytic activities, have been detected in the genomes and transcriptomes of beetles (Coleoptera) including powder-post beetles in the Bostrichoidea superfamily, to which N. hirtum and other anobiid species belong [2, 16]. With respect to the notable activity of N. hirtum larvae to degrade mannan, several endogenous genes in beetles [2, 16, 39,40,41,42] and genes in their gut-resident bacteria [12, 31, 43, 44] encoding PCWDEs with putative mannan-degrading activities (e.g., β-mannosidase and endo-β-1,4-mannanase) have been reported. It is conceivable that these PCWDEs secreted from the beetle host and/or its gut-resident microbial symbionts enable N. hirtum larvae to efficiently digest cellulose and hemicelluloses, especially mannans, for assimilation and energy.

To efficiently degrade cell wall polysaccharides, N. hirtum larvae must overcome the lignin barrier to allow PCWDEs to contact the embedded polysaccharide substrates. The digested lignocellulose diets showed markedly increased (> 70%) lignin contents, alongside proportional decreases in polysaccharide contents (Table 1 and Fig. 3). This result indicates that, as reported in other lignocellulose digestion experiments with other lignocellulose-feeding insects [10, 11, 15, 45, 46], polysaccharide decomposition exceeds lignin decomposition and a large proportion of lignin polymers remains intact in the digestive residues. Nevertheless, our in-depth lignin structural analyses, primarily by 2D HSQC NMR, detected several notable signatures indicative of partial structural modifications of lignin, including potential cleavages of the lignin polymer backbone, after its passage through the N. hirtum larvae digestive system.

For both the softwood (pine) and hardwood (Shorea) diets, the oxidized G and S lignin units ( and ) were proportionally increased over the non-oxidized G and S lignin units (G and S) after digestion (Fig. 3). We also detected proportional decreases of the major β–O–4 linkage type (I) over the other major lignin inter-monomeric linkage types (II and III). More indicatively, we detected augmentations of the aldehyde (IVʹ and IVʹʹ) and HPV/HPS (IVʹʹ) end-unit structures (Fig. 4), both of which are implicated in oxidative and/or reductive lignin biodegradation pathways as further discussed below. These lignin structural modifications, which are presumably facilitated by gut symbionts, could contribute to the deconstruction of the lignin barrier and the subsequent liberation and digestion of cellulose and hemicelluloses in the gut digestive system. A recent lignocellulose structural study conducted using 2D NMR and Py-GC/MS techniques also detected increased contents of aldehyde and HPV/HPS lignin end-unit structures in wheat straw biomass digested by termite gut microbiomes [13]. Analogous lignin degradation mechanisms may be involved in the beetle digestive system studied here.

The HPV/HPS structures in lignin have been documented in lignin degraded by β-etherases, which catalyze the reductive cleavage of the β–O–4 linkages under anaerobic conditions [47, 48]. Functional lignin β-etherases have been characterized from several ligninolytic bacterial strains [49,50,51,52], and homologous β-etherase genes are widely distributed in bacteria [53, 54], including those present as symbionts in the beetle gut [12]. To date, however, the activities of these β-etherases have been tested mostly in reactions with dimeric lignin substrates [49,50,51], whereas an earlier study detected HPV/HPS compounds released from polymeric lignin substrates treated with the β-etherase pathway enzymes in vitro [52]. On the other hand, it has been also documented that these HPV/HPS structures in lignin can arise from oxidative lignin degradation pathways of white rot fungi under aerobic conditions [33, 55, 56]. As further discussed below, previous research suggests that beetle digestive tracts have steep oxygen gradients allowing for the coexistence of aerobic and anaerobic micro-organisms in different gut compartments [57]. Thus, it is conceivable that HPV/HPS lignin structures can be generated by both reductive and oxidative lignin degradation pathways in insect digestive systems.

The aldehyde end-unit structures in lignin are typical consequences of oxidative lignin degradation and are commonly observed in lignin materials biodegraded by wood-decaying fungi [33, 58]. Previous lignocellulose structural studies primarily using Py-GC/MS detected signatures of lignin side-chain oxidation by the wood-feeding beetles, Anoplophora glabripennis (Cerambycidae) [9] and Odontotaenius disjunctus (Passalidae) [12]. Lignin side-chain oxidation has also been observed after digestion of lignin by several termite species as revealed by Py-GC/MS and/or 2D NMR [9,10,11, 13]. Although the oxidative lignin degradation pathways of fungi harboring strong lignin oxidases (e.g., lignin peroxidases and laccases) have been well documented [59], it is currently unclear whether such intensive oxidation reactions occur in insect gut digestive systems. Notably, however, a recent metagenomic study on the digestive tract of the wood-feeding beetle Odontotaenius disjunctus detected several bacterial genes encoding enzymes associated with oxidative lignin degradation, such as laccases, peroxidases, and dioxygenases. They were found to be particularly enriched in the midgut and posterior hindgut regions, which provide relatively aerobic environments with oxygen supplied from the trachea, making them suitable for oxidative degradation of lignocellulose [12]. Similar gut anatomical properties and assemblies of microbial communities may also play important roles in lignin degradation in the N. hirtum larvae digestive system.

Conclusions

Our results show that the larvae of the Anobiid beetle N. hirtum are able to deconstruct lignocellulose in their gut digestive system. The larvae can subsist on plain softwood and hardwood lignocelluloses, utilizing cellulose and hemicelluloses, especially mannans, for assimilation and energy. Such efficient polysaccharide digestion could be achieved by overcoming lignin barriers through structural modifications of lignin polymers via reductive and/or oxidative degradation pathways. Further studies should profile gut microbial communities and conduct functional analyses of the endogenous and symbiotic genes/enzymes potentially involved in lignocellulose deconstruction. The results of such studies will shed light on the unique digestive physiology that has developed and evolved among wood-feeding insects. Such information may be used to develop technologies to prevent the destruction of woody materials by these notorious pests and develop new strategies for the sustainable utilization of lignocellulosic biomass.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

ANOVA:

Analysis of variance

CWR:

Cell wall residue

DMSO:

Dimethyl sulfoxide

G:

Guaiacyl

GH:

Glycoside hydrolase

HPS:

Hydroxypropiosyringone

HPV:

Hydroxypropiovanilone

HSD:

Honestly significant difference

HSQC:

Heteronuclear single quantum coherence

NMR:

Nuclear magnetic resonance

PCWDE:

Plant cell wall-degrading enzyme

Py-GC/MS:

Pyrolysis-gas chromatography/mass spectrometry

S:

Syringyl

2D:

Two-dimensional

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Acknowledgements

The authors thank Dr. Hironori Kaji and Ms. Ayaka Maeno (ICR, Kyoto University) for their support in NMR experiments, Mr. Akio Adachi (RISH, Kyoto University) for technical assistance, and Dr. Didi Tarmadi and Dr. Khoirul Himmi S. (Research Center for Biomaterials, BRIN) for helpful discussions. Part of this study was conducted using the facilities in the DOL and DASH/ FBAS at RISH, Kyoto University and the NMR spectrometer at the JURC of ICR, Kyoto University.

Funding

This study was supported in part by the Japan Society for the Promotion of Science KAKENHI Grants (JP20H03044). NPRAK acknowledges a Ph.D. scholarship from MEXT of Japan.

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NPRAK, YT, TU, and TY conceived the research. NPRAK, YT, TM, IF and TY designed experiments. NPRAK, YT, TM, and TY performed experiments and analyzed data with TK, TH, and TU. NPRAK and YT wrote the manuscript with help from all other authors. All authors read and approved the final manuscript.

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Correspondence to Yuki Tobimatsu.

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Krishanti, N.P.R.A., Tobimatsu, Y., Miyamoto, T. et al. Structural basis of lignocellulose deconstruction by the wood-feeding anobiid beetle Nicobium hirtum. J Wood Sci 68, 10 (2022). https://doi.org/10.1186/s10086-022-02017-6

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Keywords

  • Anobiid
  • Death-watch beetle
  • Lignocellulose
  • Nicobium hirtum
  • Nuclear magnetic resonance
  • Wood-feeding insect
  • Xylophagous insects