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Journal of Wood Science

Official Journal of the Japan Wood Research Society

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Electro-oxidation of synthetic lignin with different mediators for the laccase mediator system (3-hydroxyanthranilic acid, 4-hydroxybenzoic acid, phenol red)

Journal of Wood Science volume 70, Article number: 23 (2024) Cite this article

Abstract

Three mediators for the laccase mediator system, 3-hydroxyanthranilic acid (3-HAA), 4-hydroxybenzoic acid (HBA), and phenol red (PR) were investigated as mediators in an electrolytic mediator system (EMS) for the degradation of guaiacyl synthetic lignin (G-DHP). All the electron-oxidations of G-DHP with 3-HAA, HBA and PR in the absence of 2,6-lutidine proceeded to give the electrolysis residues in moderate yields. The significant β-β and β-5 linkage loss was found in all the electrolysis residues, especially the residue in the electro-oxidation with PR was significant. The addition of 2,6-lutidine as a base increased the yields of the electrolysis residues and influenced the relative ratio of β-O-4, β-5 and β-β linkages to some extent, that is, increase of β-O-4 linkage loss and decrease of β-β linkages loss (in the electro-oxidation with 3-HAA), increase of β-O-4 linkage loss (in that with 3-HBA), increase of β-5 linkages loss (in that with PR at 0.35 V) and decrease of β-O-4 and β-β linkages loss (in that with PR at 0.70 V). Thus, the base such as 2,6-lutidine was also one of the critical factors for reaction efficiency and reaction selectivity in the EMS. Consequently,3-HAA, HBA, and PR could be used as mediators in EMS for lignin degradation, especially 3-HAA is the most preferable because of the low applied potential.

Introduction

Lignin is the most abundant aromatic polymer in nature and has a complex structure with various linkages between monolignols such as β-O-4, β-5, and β–β linkages. However, lignin is greatly underutilized as a material because the most common use of lignin is the thermal recycling of black liquor in kraft pulping. One of the important lignin uses is the conversion of lignin into useful low molecular weight aromatic compounds such as vanillin [1,2,3,4]. However, harsh reaction conditions are required in the present lignin degradation method such as kraft pulping and alkali–nitrobenzene oxidation. Therefore, lignin degradation under mild conditions is strongly desired.

By contrast, lignin is degraded at ambient temperature and pressure by white-rot fungi in nature. It is known that a low molecular weight compound, called a “mediator”, participates in the reaction between the enzyme of white-rot fungi such as laccase, peroxidase, and lignin, and an oxidized mediator (an activated mediator) is the main actual reactive species in the degradation [5]. Thus, the laccase mediator system (LMS) has been proposed and studied for many years as an eco-friendly lignin degradation system that mimics the lignin degradation reaction of white-rot fungi [6,7,8,9,10,11]. Many mediators such as N-hydroxyphthalimide (NHPI), 1-hydroxybenzotriazol, violuric acid, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and 2,2,6,6-tetramethylpiperidine-1-oxyl radical have been investigated in LMS. Phenolic compounds such as 3-hydroxyanthranilic acid (3-HAA), 4-hydroxybenzoic acid (HBA), and phenol red (PR) have been also reported as laccase mediators [7]. However, LMS is still not a practical process for industrial applications, mainly owing to the use of costly enzymes and the limitations of the reaction conditions.

Recently, an electrolytic mediator system (EMS) has been proposed and studied as an alternative eco-friendly lignin degradation system to overcome the disadvantage of LMS [12,13,14,15,16,17,18]. The mediator is one of the critical factors that influence the reaction efficiency and selectivity in EMS, but the knowledge of mediators in EMS is still significantly insufficient. For example, the promising meditators for LMS were also candidates for them in EMS, but it has been reported that the reaction behavior in EMS was different from that in LMS even though the same mediator was used [19]. Therefore, it is essential that the re-investigation of the meditators for LMS in EMS. In this study, EMS of guaiacyl dehydrogenation polymer (G-DHP) using phenolic LMS mediators, namely, 3-HAA, HBA, and PR was investigated to collect basic knowledge of mediators in EMS, because the three mediators have not been investigated as mediators in EMS although they are interesting as follows. 3-HAA is known to be a secondary metabolite of the white-rot fungus Picnoporus cinnabarinus [20], and it has been effective as a redox mediator for the oxidation of autohydrolysis lignin [21] and for the decolorization of dye [22]. HBA is also known to be a natural mediator as well as 3-HAA and mediated the oxidation of polyaromatic hydrocarbons in LMS [23]. PR has been reported to be a laccase mediator although it was a synthetic mediator and effective in the oxidation of a non-phenolic substrate [24]. In addition, the influence of 2,6-lutidine as a base in the EMS was also investigated.

Materials and methods

Materials

The mediators and substrates used in the current study are shown in Fig. 1. 3-HAA, HBA, and PR were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), Tokyo Chemical Industry (Tokyo, Japan), and Nacalai Tesque Inc. (Kyoto, Japan), respectively. G-DHP was prepared using horseradish peroxidase catalyzed dehydrogenative polymerization of coniferyl alcohol as described previously [17].

Fig. 1
figure 1

Mediators used in the current study

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Cyclic voltammetry

Cyclic voltammetry (CV) measurements were performed with an ALS 600 E electrochemical analyzer (BAS, Tokyo, Japan) at ambient temperature at scan rate of 50 mVs−1 using an undivided cell with a 1.6 mm platinum disk working electrode, a platinum wire counter electrode, and a Ag/AgCl reference electrode in 0.1 mol/L LiClO4 in CH3CN/H2O (70/30, v/v) as an electrolyte.

Bulk electrolysis of G-DHP

Bulk electrolysis was performed according to the method reported in the previous papers [17, 18]. Briefly, bulk electrolysis was performed with an ALS 600 E electrochemical analyzer and ALS 680 C power booster (BAS) at ambient temperature using a divided cell with a 2.0 × 3.0 cm2 carbon felt (thickness 0.3 cm) working electrode, a platinum wire electrode counter electrode, and a Ag/AgCl reference electrode. G-DHP (50 mg) was electrooxidized with the mediator (2.5 mmol) in the presence and absence of 2,6-lutidine (5.0 mmol) as a base at fixed potential (3-HAA at 0.31 V and 0.10 V, HBA at 0.80 V and 0.70 V, and PR at 0.71 V and 0.35 V) in 0.1 mol/L LiClO4 in CH3CN/H2O (70/30, v/v) (20 mL) as an electrolyte with stirring. G-DHP was almost dissolved in the electrolyte before the oxidation. The reaction was stopped after 6 h. The reaction mixture in the anode chamber was concentrated in vacuo to remove CH3CN to afford a suspension. The suspension was extracted with CH2Cl2 (30 mL) three times. The combined CH2Cl2 extract solutions were concentrated in vacuo to afford a CH2Cl2-soluble fraction. The suspension after CH2Cl2 extraction was filtered to separate a filtrate (a water-soluble fraction) and a residue. The residue was washed with distilled water and then freeze-dried to give a G-DHP electrolysis residue. The bulk electrolysis was repeated three times.

NMR spectroscopy

The 2-D HSQC NMR spectra were recorded on a Varian FT-NMR (300 MHz) spectrometer (Agilent Technologies, Santa Clara, CA, USA) interfaced with the Varian VnmrJ 3.2 software. Original G-DHP and the G-DHP electrolysis residues were dissolved in DMSO-d6/pyridine-d5 (4/1) (v/v).

FT-IR spectroscopy

FT-IR spectra of original G-DHP and the G-DHP electrolysis residues were recorded on a Spectrum 3 (PerkinElmer, Shelton, CT, USA) using KBr pellets. The obtained FT-IR spectra were normalized to the band at 1500 cm−1 derived from aromatic skeletal vibrations of the G-aromatic nuclei.

GPC

The original G-DHP and the G-DHP electrolysis residues were acetylated with Ac2O/pyridine at ambient temperature for 3 days before the measurements. The measurements were performed using a Shimadzu LC-10 system (Shimadzu Co., Kyoto, Japan) equipped with a Shimadzu UV detector (SPD-10Avp) under the following conditions: columns: K-802, K-802.5, and K-805 (Showa Denko K.K., Tokyo, Japan) connected in series, column temperature: 40 °C, eluent: CHCl3, flow rate: 1.0 mL/min, detection wavelength: 254 nm, standards: polystyrene (Showa Denko K.K.).

LC–MS

The CH2Cl2-soluble fractions were subjected to LC–MS measurements. The measurements were performed using a Shimadzu LC-20AD system (Shimadzu) equipped with Shimadzu SPD-20A and LC-MS2020 detectors (Shimadzu) under the following conditions: column: Asahipak ODP-50 4E (Showa Denko K.K.), column temperature: 40 °C, eluent: A (water) and B (CH3CN) gradient mode 0 min 20% B, 42 min 75% B, 44–49 min 95% B, 50–70 min 20% B, flow rate: 0.3 mL/min, detection wavelength: 254 nm, ionic method: ESI.

Results and discussion

Determination of applied potentials by CV

Cyclic voltammograms of the mediators alone are shown in Fig. 2a–c. The oxidation peak of 3-HAA was clearly observed at 0.31 V, whereas its reduction peak was done at 0.20 V as a shoulder peak. The oxidation and reduction peaks of HBA were not clearly observed, and only the oxidation peak of PR was observed at 0.71 V. It has been reported that 2,6-lutidine decreases the oxidation potential of a phenolic mediator such as mesitol [25]. Cyclic voltammograms of the mediators in the presence of 2,6-lutidine are shown in Fig. 2d–f. In all the voltammograms, the peak patterns were significantly different from those in the voltammograms of the mediators alone, and the oxidation peaks shifted to low potentials as expected although the reduction peaks were not clearly observed. The oxidation peaks of 3-HAA, HBA, and PR were observed at 0.10, 0.70, and 0.35 V, respectively. The applied potentials for bulk electrolysis were determined from the oxidation potentials of the mediators in the absence and presence of 2,6-lutidine. However, the applied potential of HBA in the absence of 2,6-lutidine was set midway (0.80 V) between the potential of the rising point of the current (0.6 V) and that of the end potential (1.0 V).

Fig. 2
figure 2

Cyclic voltammograms of the mediators in the absense (a–c) and presence (d–f) of 2,6-lutidine (electrolyte: 0.1 M LiClO4 in CH3CN/H2O/(70/30, v/v); scan rate: 50 mVs−1)

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Bulk electrolysis of G-DHP with the mediators

Three types of bulk electrolysis of G-DHP were carried out, that is, the electrolysis with mediators in the absence of 2,6-lutidine at the high oxidation potentials determined by the CVs in Fig. 2a–c, and those in the presence of 2,6-lutidine at the high and low oxidation potentials determined by the CVs in Fig. 2a–c and d–f, respectively. The reaction mixtures in the anode chamber including G-DHP and the mediators before electrolysis were weakly acidic in the electro-oxidation with 3-HAA and HBA in the absence of 2,6-lutidine, and that in the electro-oxidation with PR in the absence of 2,6-lutidine was acidic. On the other hand, the reaction mixtures in the electro-oxidation in the presence of 2,6-lutidine was neutral (Table S1). HSQC NMR spectra and the semiquantitative analysis of the NMR signals of all the electrolysis residues are summarized in Fig. 3 and Table 1, respectively.

Fig. 3
figure 3

HSQC NMR spectra of electrolysis residues from G-DHP, top (aliphatic region), top inset (aldehyde region), bottom (aromatic region)

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Table 1 Results of the electro-oxidation of G-DHP with 3-HAA, HBA and PR
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3-HAA

The electro-oxidation of G-DHP with 3-HAA in the absence of 2,6-lutidine at 0.31 V proceeded to afford an electrolysis residue (G-DHP/3-HAA) in 39% yield. The molecular weight of G-DHP/3-HAA was lower than that of the G-DHP original, although the appearance of broad peak in GPC chromatogram (Figure S1) suggested recondensation of G-DHP. Signals IIβ, IIIα, IIIβ, and IIIγ disappeared, and signals Iα, Iβ, Iγ, IIα, and IIγ decreased in the HSQC NMR spectrum of G-DHP/3-HAA (I: β-O-4, II: β-5, III: β-β substructures, IV: coniferyl alcohol end unit, V: coniferyl aldehyde end unit, G: guaiacyl unit), suggesting that β-β and β-5 linkages were lost significantly in the electro-oxidation. Signals IV and V were not found in the HSQC spectrum in the aldehyde region. Cγ-oxidation, which are often observed in the electro-oxidation of G-DHP with other non-phenolic mediators [16, 17], did not proceed. There was no significant difference between the FT-IR spectra of G-DHP and G-DHP/3-HAA (Figure S2). On the other hand, the electro-oxidation of G-DHP with 3-HAA in the presence of 2,6-lutidine at 0.31 V and 0.10 V proceeded to afford electrolysis residues (G-DHP/3-HAA-L1, G-DHP/3-HAA-L2) in 61% and 58% yields, respectively. The molecular weights of G-DHP/3-HAA-L1 and -L2 were also lower than those of the G-DHP original. Signals Iα, Iβ, Iγ, IIα, IIβ, IIγ, IIIα, IIIβ, and IIIγ were decreased in the HSQC NMR spectra of G-DHP/3-HAA-L1 and -L2. The reaction trend of the electro-oxidation was changed by the addition of 2,6-lutidine; briefly, β-β linkages loss was decreased.

The electrolyte after the electro-oxidation was extracted with CH2Cl2. The yields of CH2Cl2-soluble fractions were low (less than 10%), whereas the estimated water-soluble fractions were high (Table S1). However, the extract solvent was limited, because the supporting salt (LiClO4) is soluble in common organic solvents such as ethyl acetate, acetone. The CH2Cl2-soluble fractions in the electro-oxidation with 3-HAA in the absence and presence of 2,6-lutidine were analyzed using LC–MS analysis. The LC chromatogram of the CH2Cl2-soluble fraction and ESI–MS spectrum (negative mode) of the peak at a retention time of 24.2 min in the electro-oxidation with 3-HAA in the absence of 2,6-lutidine are shown in Figs. 4 and 5a, respectively. The peak at m/z = 151 derived from vanillin (Figure S3) was found in the latter spectrum, suggesting that Cα–Cβ cleavage occurred in the electro-oxidation (Fig. 5a, d, g). Vanillin was detected in all the CH2Cl2-soluble fractions in the electro-oxidation with 3-HAA. The analysis of the water-soluble fractions in the electro-oxidation with 3-HAA was also tried, but it was difficult to separate the supporting salt (LiClO4).

Fig. 4
figure 4

LC chromatograms of G-DHP/3-HAA CH2Cl2-soluble fraction

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Fig. 5
figure 5

ESI–MS spectra (negative mode) of the peak at 24.2 min in LC chromatograms of the CH2Cl2-soluble fractions

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HBA

The electro-oxidation of G-DHP with HBA in the absence of 2,6-lutidine at 0.80 V proceeded to afford an electrolysis residue (G-DHP/HBA) in 40% yield. The molecular weight of G-DHP originally decreased in the electro-oxidation. Signals Iβ, IIβ, IIIα, IIIβ, and IIIγ disappeared, and signals IIα, IIγ, Iα, and Iγ were decreased in the HSQC NMR spectrum of G-DHP/HBA (Fig. 3). Signals IV and V were not found in the aldehyde region. The reaction trend in the electro-oxidation with HBA were similar to those in the electro-oxidation with 3-HAA; that is, β-β and β-5 linkages were lost significantly in the electro-oxidation. The electro-oxidation of G-DHP with HBA in the presence of 2,6-lutidine at 0.80 V and 0.70 V proceeded to afford electrolysis residues (G-DHP/HBA-L1, G-DHP/HBA-L2) in 45% and 58% yields, respectively. The molecular weight of G-DHP/HBA-L1 was lower than that of the G-DHP original, but that of G-DHP/HBA-L2 was higher. Although the difference in the molecular weights of the electrolysis residues cannot be explained from the present data, it suggested that the applied potential was one of the important factors in the electro-oxidation. The reaction trend of the electro-oxidation with HBA was influenced by the addition of 2,6-lutidine (β-O-4 linkages loss increased). Vanillin was also detected in all the CH2Cl2-soluble fractions in the electro-oxidation with HBA (Fig. 5b, e, h).

PR

The electro-oxidation of G-DHP with PR in the absence of 2,6-lutidine at 0.71 V proceeded to afford an electrolysis residue (G-DHP/PR) in 44% yield. The molecular weight of G-DHP/PR was much lower than that of the G-DHP original. Signals IIα and IIIα disappeared and signals Iα, Iβ, Iγ, IIβ, IIγ, IIIβ, and IIIγ decreased in the HSQC NMR spectrum of G-DHP/PR (Fig. 3). The reaction trend in the electro-oxidation with PR were somewhat different from those in the electro-oxidation with 3-HAA and HBA, that is, β-5 and β-β linkages were lost significantly in the electro-oxidation. The electro-oxidation of G-DHP with PR in the presence of 2,6-lutidine at 0.71 V and 0.35 V proceeded to afford electrolysis residues (G-DHP/PR-L1, G-DHP/PR-L2) in 64% and 68% yields, respectively. The molecular weights of G-DHP/PR-L1 and -L2 were slightly lower than that of the G-DHP original. The reaction trend of the electro-oxidation with PR was changed by the addition of 2,6-lutidine and applied potential; briefly, the β-5 linkages and β-β linkages were significantly lost at the electro-oxidation at 0.35 V and 0.71 V, respectively. Vanillin was also detected in all the CH2Cl2-soluble fractions in the electro-oxidation with PR (Fig. 5c, f, i). Thus, the reaction trend was influenced by mediators, a base (2,6-lutidine) and applied potential, although they could not be explained from the present data.

Conclusion

Laccase mediators (3-HAA, HBA, and PR) were investigated as mediators in the electro-oxidation of G-DHP. First, the electro-oxidation with 3-HAA at 0.31 V in the absence of 2,6-lutidine proceeded and showed significant β-β and β-5 linkage loss. The electro-oxidation can be performed even at a low applied potential (0.10 V) in the presence of 2,6-lutidine. 2,6-Lutidine prevented the electro-oxidation to some extent and prevented the loss of β-β linkages of G-DHP under the present conditions. Second, the electro-oxidation with HBA in the absence of 2,6-lutidine proceeded and showed significant β-β and β-5 linkage loss as well as the electro-oxidation with 3-HAA. 2,6-Lutidine prevented the reaction to some extent and influenced the reaction trend, that is, promoting the loss of β-O-4 linkages. Third, the electro-oxidation with PR showed significant β-5 and β-β linkage losses in the absence of 2,6-lutidine. 2,6-Lutidine prevented the loss of β-5 linkages and β-β linkages to some extent at 0.35 V and 0.71 V, respectively. Consequently, 3-HAA, HBA, and PR could be used as mediators in EMS, because the yields of the electrolysis residues in the electro-oxidation with the three mediators was corresponded to those in the electro-oxidation with NHPI and ABTS [17]. Especially 3-HAA was the most promising mediator because the applied potential was low. However, further investigations are required to explain the influence of the mediators and 2,6-lutidine on the reaction efficiency and selectivity in the electro-oxidation.

Availability data and materials

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Abbreviations

CV:

Cyclic voltammetry

EMS:

Electrolytic mediator system

G-DHP:

Guaiacyl dehydrogenation polymer

3-HAA:

3-Hydroxy anthranilic acid

HBA:

4-Hydroxybenzoic acid

LMS:

Laccase mediator system

PR:

Phenol red

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Acknowledgements

The authors gratefully acknowledged Prof. Hiroshi Kamitakahara (Graduate School of Agriculture, Kyoto University) for the assist of LC–MS measurements.

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Authors and Affiliations

  1. Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-Cho, Sakyo-Ku, Kyoto, 606-8502, Japan

    Shin Tanahashi, Bing Xie, Yoshikuni Teramoto & Toshiyuki Takano

  2. Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan

    Bing Xie

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  1. Shin Tanahashi
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  4. Toshiyuki Takano
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Contributions

ST contributed to all experiments. BX and YT supported to ST’s experiments. TT (corresponding author) designed this study and wrote this paper with ST.

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Correspondence to Toshiyuki Takano.

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Tanahashi, S., Xie, B., Teramoto, Y. et al. Electro-oxidation of synthetic lignin with different mediators for the laccase mediator system (3-hydroxyanthranilic acid, 4-hydroxybenzoic acid, phenol red). J Wood Sci 70, 23 (2024). https://doi.org/10.1186/s10086-024-02137-1

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